HW1822 |
lin-29(xe61[lin-29::gfp::3xflag]) II |
Low penetrance Pvl (i.e., tagged protein appears not fully functional). CRISPR/Cas9-engineered allele adds GFP and 3xflag tag to LIN-29. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Reference: Aeschimann F, et al., Mol Cell. 2017 Feb 2;65(3):476-489. |
HW1826 |
lin-29(xe63[gfp::3xflag::lin-29a]) II. |
Superficially wild-type. CRISPR/Cas9-engineered allele adds GFP and 3xflag tag to the N-terminus of LIN-29A. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Reference: Aeschimann F, et al., (2019). A single let-7 target to coordinate transition to adulthood. Life Science Alliance 2, e201900335. http://dx.doi.org/10.26508/lsa.201900335 ) |
HW1835 |
lin-29(xe40 xe65[lin-29b::gfp::3xflag]) II. |
Partially penetrant Pvl and Egl. xe40 specifically disrupts the LIN-29A isoform. The GFP tag was inserted at the shared C-terminus of LIN-29A/B in the xe40 background, so only the labeled B isoform is expressed. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Reference: Pereira et al., (2019) Timing mechanism of sexually dimorphic nervous system differentiation. eLIFE 8: e42078. https://doi.org/10.7554/eLife.42078 |
MT1077 |
lin-29(n482) II. |
Egg laying defective. Makes bags of worms. Abnormal vulva. Previously called egl-29. |
MT1727 |
dpy-10(e128) lin-29(n482) II. |
Dpy. Egg laying defective. Makes bags of worms. Abnormal vulva. Previously called egl-29(n482). |
MT333 |
lin-29(n333) II. |
Vulvaless. |
NOA4 |
sqt-1(sc13) lin-29(n333) II; lin-12(n137n460) III. |
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RG93 |
lin-29(ve5) rol-1(e91)/mnC1 [dpy-10(e128) unc-52(e444)] II. |
Heterozygotes are WT and segregate WT, Egls which have a protruding vulva, and DpyUncs. Maintain by picking WT. lin-29 suppresses rol-1 phenotype (rol-1 is an adult specific Roller and lin-29 animals never molt to adult cuticle). |
RW12227 |
lin-29(st12227[lin-29::TY1::EGFP::3xFLAG]) II. |
CRISPR/Cas9 engineered tagged endogenous locus. |
VT132 |
sqt-1(sc13) lin-29(n333)/mnC1 [dpy-10(e128) unc-52(e444)] II. |
Heterozygotes are slightly shorter than WT and segregate more animals which are slightly shorter than WT, Rollers which are Egl and have a protruding vulva, and DpyUncs. |
VT516 |
lin-29(n546)/mnC1 [dpy-10(e128) unc-52(e444)] II. |
Heterozygotes are slightly shorter than WT and segregate DpyUnc and Egl. |