Gene Information: lin-29

Namelin-29 View on WormBase
Species C. elegans
SequenceW03C9.4
Genetic positionII:4.14 +/- 0.002 cM
Genomic positionII: 11917642..11935343

Strains carrying this gene

Strain Genotype Description
HW1822 lin-29(xe61[lin-29::gfp::3xflag]) II Low penetrance Pvl (i.e., tagged protein appears not fully functional). CRISPR/Cas9-engineered allele adds GFP and 3xflag tag to LIN-29. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Reference: Aeschimann F, et al., Mol Cell. 2017 Feb 2;65(3):476-489.
HW1826 lin-29(xe63[gfp::3xflag::lin-29a]) II. Superficially wild-type. CRISPR/Cas9-engineered allele adds GFP and 3xflag tag to the N-terminus of LIN-29A. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Reference: Aeschimann F, et al., (2019). A single let-7 target to coordinate transition to adulthood. Life Science Alliance 2, e201900335. http://dx.doi.org/10.26508/lsa.201900335 )
HW1835 lin-29(xe40 xe65[lin-29b::gfp::3xflag]) II. Partially penetrant Pvl and Egl. xe40 specifically disrupts the LIN-29A isoform. The GFP tag was inserted at the shared C-terminus of LIN-29A/B in the xe40 background, so only the labeled B isoform is expressed. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Reference: Pereira et al., (2019) Timing mechanism of sexually dimorphic nervous system differentiation. eLIFE 8: e42078. https://doi.org/10.7554/eLife.42078
MT1077 lin-29(n482) II. Egg laying defective. Makes bags of worms. Abnormal vulva. Previously called egl-29.
MT1727 dpy-10(e128) lin-29(n482) II. Dpy. Egg laying defective. Makes bags of worms. Abnormal vulva. Previously called egl-29(n482).
MT333 lin-29(n333) II. Vulvaless.
NOA4 sqt-1(sc13) lin-29(n333) II; lin-12(n137n460) III.
RG93 lin-29(ve5) rol-1(e91)/mnC1 [dpy-10(e128) unc-52(e444)] II. Heterozygotes are WT and segregate WT, Egls which have a protruding vulva, and DpyUncs. Maintain by picking WT. lin-29 suppresses rol-1 phenotype (rol-1 is an adult specific Roller and lin-29 animals never molt to adult cuticle).
RW12227 lin-29(st12227[lin-29::TY1::EGFP::3xFLAG]) II. CRISPR/Cas9 engineered tagged endogenous locus.
VT132 sqt-1(sc13) lin-29(n333)/mnC1 [dpy-10(e128) unc-52(e444)] II. Heterozygotes are slightly shorter than WT and segregate more animals which are slightly shorter than WT, Rollers which are Egl and have a protruding vulva, and DpyUncs.
VT516 lin-29(n546)/mnC1 [dpy-10(e128) unc-52(e444)] II. Heterozygotes are slightly shorter than WT and segregate DpyUnc and Egl.