Gene Information: nhr-67

Namenhr-67 View on WormBase
Species C. elegans
SequenceC08F8.8
Genetic positionIV:4.92 +/- 0.001 cM
Genomic positionIV: 11169748..11174791

Strains carrying this gene

Strain Genotype Description
LA275 nhr-67(pf2) IV. Pvl. Egl. Weaker allele than pf88 and pf159.
LA691 nhr-67(pf159) IV. Pvl. Egl. Poor mating.
MU1255 nhr-67(tm2217) IV/nT1 [qIs51] (IV;V). Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP tm2217 homozygotes (arrested L1 larvae). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain.
MU1269 nhr-67(pf88) IV/nT1 [qIs51] (IV;V). Heterozygotes are WT and GFP+. Pick GFP+ to maintain -- viable pf88 homozygotes (Pvl Egl) can overtake the bablanced population. nT1[qIs51] is homozygous lethal. qIs51 is an insertion of ccEx9747 with markers: myo-2::GFP expressed in the pharynx throughout development, pes-10::GFP expressed in the embryo, and a gut promoter F22B7.9::GFP expressed in the intestine. Reference: Verghese E, et al. Dev Biol. 2011 Aug 15;356(2):516-28.
MU1289 nhr-67(pf88) IV. Pvl. Egl. Poor mating.
OH14711 nhr-67(ot795) IV; him-5(e1490) V; otEx5999. otEx5999 [nhr-67(fosmid) + unc-47p::mChopti]. Him. ot795 is lethal after L1 stage; all animals L2 and older should carry the extra-chromosomal array. otEx5999 carries fosmid WRM0613bE08; reporter contains 300 bp upstream of the unc-47 start codon. Reference: Gendrel M, et al. Elife. 2016 Oct 14;5.
OH14712 nhr-67(ot795) IV; him-5(e1490) V; otEx6001. otEx6001 [nhr-67(fosmid) + unc-47p::GFP]. Him. ot795 is lethal after L1 stage; all animals L2 and older should carry the extra-chromosomal array. otEx6001 carries fosmid WRM0613bE08; reporter contains 300 bp upstream of the unc-47 start codon. Reference: Gendrel M, et al. Elife. 2016 Oct 14;5.
OH3926 otIs114 I; nhr-67(ot136) IV. otIs114 [lim-6p::GFP + rol-6(su1006)]. Shows ASE asymmetry defects. Mildly temperature sensitive. Maintain at 25C.
OH3928 otIs114 I; nhr-67(ot202) IV. otIs114 [lim-6p::GFP + rol-6(su1006)]. ASE asymmetry defects. Mild temperature sensitivity; maintain at 25 C.
OH4025 otIs114 I; nhr-67(ot190) IV. otIs114 [lim-6p::GFP + rol-6(su1006)]. Shows ASE asymmetry defects.
OH7566 mgIs18 unc-24(e138) nhr-67(ot407) IV; ntIs1 V; otEx3103. mgIs18 [ttx-3p::GFP] IV. ntIs1 [gcy-5p::GFP + lin-15(+)] V. otEx3103 [elt-2::GFP]. All worms have mgIs18 and ntIs1. mgIs18 labels AIY interneurons. ntIs1 marks one GFP+ neuron (ASER) in the head. otEx3103 [elt-2::GFP] is expressed in intestinal cells; maintain by picking animals with intestinal GFP. Worms that have lost the array are Emb, Lvl, and have an ASE defect.
OH7631 nhr-67(ok631) IV; otEx3362. otEx3362 [nhr-67(fosmid)::mCherry + elt-2::GFP]. Pick GFP+ to maintain. otEx3362 rescues nhr-67(ok631).
OH7726 otIs114 I; nhr-67(ot158) IV. otIs114 contains [lim-6p::GFP + rol-6(su1006)]. Rollers.
PHX509 nhr-67(syb509[nhr-67::gfp]) IV. GFP reporter inserted into C-terminus of endogenous nhr-67 locus. Reference: Medwig-Kinney TN, et al. Development. 2020 Jan 2;147(1).
VC419 nhr-67(ok631) IV/nT1 [qIs51] (IV;V). C08F8.8. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1 aneuploid progeny, and GFP- ok631 homozygotes (Unc, probable L1 arrest). nT1[qIs51] homozygotes inviable. Pick GFP+ WT and check for correct segregation of progeny to maintain. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807