| LA275 |
nhr-67(pf2) IV. |
Pvl. Egl. Weaker allele than pf88 and pf159. |
| LA691 |
nhr-67(pf159) IV. |
Pvl. Egl. Poor mating. |
| MU1255 |
nhr-67(tm2217) IV/nT1 [qIs51] (IV;V). |
Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP tm2217 homozygotes (arrested L1 larvae). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. |
| MU1269 |
nhr-67(pf88) IV/nT1 [qIs51] (IV;V). |
Heterozygotes are WT and GFP+. Pick GFP+ to maintain -- viable pf88 homozygotes (Pvl Egl) can overtake the bablanced population. nT1[qIs51] is homozygous lethal. qIs51 is an insertion of ccEx9747 with markers: myo-2::GFP expressed in the pharynx throughout development, pes-10::GFP expressed in the embryo, and a gut promoter F22B7.9::GFP expressed in the intestine. Reference: Verghese E, et al. Dev Biol. 2011 Aug 15;356(2):516-28. |
| MU1289 |
nhr-67(pf88) IV. |
Pvl. Egl. Poor mating. |
| OH14711 |
nhr-67(ot795) IV; him-5(e1490) V; otEx5999. |
otEx5999 [nhr-67(fosmid) + unc-47p::mChopti]. Him. ot795 is lethal after L1 stage; all animals L2 and older should carry the extra-chromosomal array. otEx5999 carries fosmid WRM0613bE08; reporter contains 300 bp upstream of the unc-47 start codon. Reference: Gendrel M, et al. Elife. 2016 Oct 14;5. |
| OH14712 |
nhr-67(ot795) IV; him-5(e1490) V; otEx6001. |
otEx6001 [nhr-67(fosmid) + unc-47p::GFP]. Him. ot795 is lethal after L1 stage; all animals L2 and older should carry the extra-chromosomal array. otEx6001 carries fosmid WRM0613bE08; reporter contains 300 bp upstream of the unc-47 start codon. Reference: Gendrel M, et al. Elife. 2016 Oct 14;5. |
| OH3926 |
otIs114 I; nhr-67(ot136) IV. |
otIs114 [lim-6p::GFP + rol-6(su1006)]. Shows ASE asymmetry defects. Mildly temperature sensitive. Maintain at 25C. |
| OH3928 |
otIs114 I; nhr-67(ot202) IV. |
otIs114 [lim-6p::GFP + rol-6(su1006)]. Mildly cold-sensitive; maintain at 25C. ASE asymmetry defects. |
| OH4025 |
otIs114 I; nhr-67(ot190) IV. |
otIs114 [lim-6p::GFP + rol-6(su1006)]. Shows ASE asymmetry defects. |
| OH7566 |
mgIs18 unc-24(e138) nhr-67(ot407) IV; ntIs1 V; otEx3103. |
mgIs18 [ttx-3p::GFP] IV. ntIs1 [gcy-5p::GFP + lin-15(+)] V. otEx3103 [elt-2::GFP]. All worms have mgIs18 and ntIs1. mgIs18 labels AIY interneurons. ntIs1 marks one GFP+ neuron (ASER) in the head. otEx3103 [elt-2::GFP] is expressed in intestinal cells; maintain by picking animals with intestinal GFP. Worms that have lost the array are Emb, Lvl, and have an ASE defect. |
| OH7631 |
nhr-67(ok631) IV; otEx3362. |
otEx3362 [nhr-67(fosmid)::mCherry + elt-2::GFP]. Pick GFP+ to maintain. otEx3362 rescues nhr-67(ok631). |
| OH7726 |
otIs114 I; nhr-67(ot158) IV. |
otIs114 contains [lim-6p::GFP + rol-6(su1006)]. Rollers. |
| PHX509 |
nhr-67(syb509[nhr-67::gfp]) IV. |
GFP reporter inserted into C-terminus of endogenous nhr-67 locus. Reference: Medwig-Kinney TN, et al. Development. 2020 Jan 2;147(1). |
| VC419 |
nhr-67(ok631) IV/nT1 [qIs51] (IV;V). |
C08F8.8. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1 aneuploid progeny, and GFP- ok631 homozygotes (Unc, probable L1 arrest). nT1[qIs51] homozygotes inviable. Pick GFP+ WT and check for correct segregation of progeny to maintain. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807 |