| KR1319 |
dpy-5(e61) nars-1(h680) unc-13(e450) I; sDp2 (I;f). |
Unc-13 phenotype. Segregates Uncs and lethal DpyUncs. h680 arrests as an early larva. Maintain by picking Unc-13 and checking for correct segregation of progeny. |
| KR440 |
dpy-5(e61) let-528(h1012) nars-1(h106) unc-13(e450) I; sDp2 (I;f). |
Unc-13 phenotype. Segregates Uncs and lethal DpyUncs (h1012 arrests as a late larva, and h106 arrests as an early larva; the double arrests as an early larva). Maintain by picking Unc-13 and checking for correct segregation of progeny. |
| KR753 |
nars-1(h428) dpy-5(e61) unc-13(e450) I; sDp2 (I;f). |
Animals with the Dup are Unc. Animals which have lost the Dup are DpyUncLet and arrest in early larval development. Maintain by picking Unc. |
| VC499 |
nars-1(ok420) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). |
F22D6.3. Homozygous lethal deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ok420 homozygotes (arrest stage/phenotype undetermined). Homozygous hT2[bli-4 let-? qIs48] inviable. Note: qIs48 has been observed to recombine off hT2, typically leaving behind a functional homozygous viable hT2 with Bli-4 phenotype. Pick WT GFP and check for correct segregation of progeny to maintain. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807 |