Gene Information: let-363

Namelet-363 View on WormBase
Species C. elegans
Genetic positionI:-0.26 +/- 0.011 cM
Genomic positionI: 5245743..5263424

Strains carrying this gene

Strain Genotype Description
KR344 let-363(h98) dpy-5(e61) unc-13(e450) I; sDp2 (I;f). Animals with the Dup are Unc. Animals which have lost the Dup are DpyUnc and arrest in late larval development. See also WBPaper00005456.
KR827 let-363(h502) dpy-5(e61) unc-13(e450) I; sDp2 (I;f). Animals with the duplication are Unc. Animals which have lost the duplication are Dpy Unc and arrest in early mid development.
VC2312 let-363(ok3018) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). B0261.2. Homozygous lethal deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ok3018 homozygotes (late larval arrest). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: TCTCCGCTCCAGCTACAAAT. External right primer: ATCGAGCTTTGCTGTTTCGT. Internal left primer: AGGGATTGATGCTGCAAGAG. Internal right primer: TTCGGAAATCGTTCCAAAAC. Internal WT amplicon: 1186 bp. Deletion size: 664 bp. Deletion left flank: GAGCCGGTATCTATCTCATCGTGTGCTTAG. Deletion right flank: ATTCGAACTCATCAGATGTGAATCCTCACA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807