| GW1203 |
met-2(n4256) set-25(n5021) III; bcIs39 [lim-7p::ced-1::GFP + lin-15(+)] V. |
Worms are slow growing, with reduced brood size and become sterile at elevated temperatures. CED-1::GFP used to detect apoptotic cells in germline. Reference: Zeller P, et al. Nat Genet. 2016 Nov;48(11):1385-1395. doi: 10.1038/ng.3672. PMID: 27668659. |
| JH3205 |
lin-15B(ax2061[lin-15B::GFP]) X. |
Maintain at 20C. Nuclear expression of lin-15B::GFP in oocytes and embryos. Reference: Paix A, et al. Genetics. 2014 Sep 23. |
| KP3948 |
eri-1(mg366) IV; lin-15B(n744) X. |
Enhanced RNAi. Temperature sensitive sterile at 25C. Grow at 15 to 20C. |
| LX1918 |
vsIs164 lite-1(ce314) lin-15B&lin-15A(n765) X. |
vsIs164 [unc-103p(E)::GCaMP5 + unc-103p(E)::mCherry + lin-15(+)] X. Integrated transgene using unc-103e promoter to drive GCaMP5 and mCherry expression in vulval muscles; useful for visualizing and quantitating calcium influx in vulval muscle cells. Reference: Collins K, et al. Elife. 2016 Nov 16;5. pii: e21126. doi: 10.7554/eLife.21126. |
| LX1960 |
lite-1(ce314) lin-15B&lin-15A(n765) X; vsIs172. |
vsIs172 [lin-11(enhancer)::pes-10p::GCaMP5 + lin-11(enhancer)::pes-10p::mCherry + lin-15(+)]. Integrated transgene using lin-11 enhancer region fused to the pes-10 basal promoter to drive GCaMP5 and mCherry expression in VC motor neurons; useful for visualizing and quantitating calcium influx in VC motor neurons. Reference: Collins K, et al. Elife. 2016 Nov 16;5. pii: e21126. doi: 10.7554/eLife.21126. |
| LX1986 |
vsIs177 lite-1(ce314) lin-15B&lin-15A(n765) X. |
vsIs177 [ocr-2p::GCaMP5::ocr-2 3'UTR + ocr-2p::mCherry::ocr-2 3'UTR + lin-15(+)] X. Integrated transgene using ocr-2 promoter to drive GCaMP5 and mCherry expression in uv1; useful for visualizing and quantitating calcium influx in uv1 cells. Reference: Collins K, et al. Elife. 2016 Nov 16;5. pii: e21126. doi: 10.7554/eLife.21126. |
| LX2004 |
vsIs183 lite-1(ce314) lin-15B&lin-15A(n765) X. |
vsIs183 [nlp-3p::GCaMP5::nlp-3 3'UTR + nlp-3p::mCherry::nlp-3 3'UTR + lin-15(+)] X. Integrated transgene using nlp-3 promoter to drive GCaMP5 and mCherry expression in HSN; useful for visualizing and quantitating calcium influx in HSN. Reference: Collins K, et al. Elife. 2016 Nov 16;5. pii: e21126. doi: 10.7554/eLife.21126. |
| MT10408 |
lin-53(n833) I; unc-76(e911) V; lin-15A(n767) X; nEx998. |
nEx998 [lin-53::GFP + unc-76(+)]. Pick non-Unc, non-Muv to maintain. |
| MT12719 |
lin-61(n3809) I; lin-15A(n767) X. |
Reference: Andersen EC, et al. Genetics. 2008 Aug;179(4):2001-12. Harrison MM, et al. Genetics. 2007 May;176(1):255-71. |
| MT15537 |
unc-30(e191) lin-54(n3423) IV/nT1 [qIs51] (IV;V); lin-15A(n767) X. |
Heterozygotes are Muv and GFP+ and segregate SteUncMuv GFP- and dead eggs. n3423 is PVul and sterile when alone; Muv in synMuv class A background. |
| MT1628 |
lin-9(n112) III; lin-15A(n749) X. |
Synthetic Muv. n749 is lin-15 Class A allele. |
| MT16429 |
set-6(tm1611) lin-15A(n767) X. |
Reference: Andersen EC & Horvitz HR., Development. 2007 Aug;134(16):2991-9. |
| MT1643 |
lin-36(n766) III; lin-15A(n767) X. |
Double mutant is Muv. lin-36 alone is non-Muv. |
| MT16430 |
set-6(tm1611) lin-15B(n744) X. |
Reference: Andersen EC & Horvitz HR., Development. 2007 Aug;134(16):2991-9. |
| MT16529 |
lin-61(n3447) I; lin-15A(n767) X. |
SynMuv B. Reference: Andersen EC, et al. Genetics. 2008 Aug;179(4):2001-12. Harrison MM, et al. Genetics. 2007 May;176(1):255-71. |
| MT1806 |
lin-15A(n767) X. |
WT phenotype. Synthetic Muv. |
| MT19703 |
nIs394 III; lin-15B&lin-15A(n765) X. |
nIs394 [ngn-1::GFP + lin-15(+)] III. Translational GFP reporter. Reference: Nakano S, et al. Development. 2010 Dec;137(23):4017-27. |
| MT2495 |
lin-15B(n744) X. |
|
| MT5828 |
lin-36(n766) unc-36(e251) III; lin-15A(n767) X. |
Unc. Muv. |
| MT664 |
lin-8(n111) II; lin-15B(n374) X. |
Synthetic Muv. |
| MT9343 |
lin-36(n766) III; lin-15A(n767) X; nIs93. |
nIs93 [(pJHT27)lin-36p::GFP::lin-36 (full-length coding) + rol-6(su1006)]. Rollers. Non-Muv -- nearly completely penetrant rescue of Lin-36. Reference: Thomas & Horvitz (1999) Development 126(15):3449-59. |
| MT990 |
lin-9(n112) III; lin-15A(n433) X. |
Synthetic Muv. n433 is lin-15 Class A allele. |
| TU3335 |
lin-15B(n744) X; uIs57. |
uIs57 [unc-119p::YFP + unc-119p::sid-1 + mec-6p::mec-6]; appears to map to LG V. Hypersensitive neuronal RNAi by feeding. Superficially wild-type. YFP detectable in neurons. Maintain 15-20 degrees; sick at 25 C. Reference: Calixto et al. (2010) Nature Methods 7:554-9. |
| TU3568 |
sid-1(pk3321) him-5(e1490) V; lin-15B(n744) X; uIs71. |
uIs71 [(pCFJ90) myo-2p::mCherry + mec-18p::sid-1]. TRN-specific RNAi by feeding. Him (~50% males). Maintain 15-20 degrees. Reference: Calixto et al. (2010) Nature Methods 7:554-9. |
| TU3595 |
sid-1(pk3321) him-5(e1490) V; lin-15B(n744) X; uIs72. |
uIs72 [pCFJ90(myo-2p::mCherry) + unc-119p::sid-1 + mec-18p::mec-18::GFP]. Hypersensitive neuronal RNAi by feeding. GFP detectable in TRNs. Him (~50% males). Maintain 15-20 degrees. Reference: Chalfie (2010) Worm Breeders Gazette. |
| TU3596 |
sid-1(pk3321) him-5(e1490) V; lin-15B(n744) X. |
Him. Enhanced RNAi background. Maintain under normal conditions. |
| VH624 |
rhIs13 V; nre-1(hd20) lin-15B(hd126) X. |
rhIs13 [unc-119::GFP + dpy-20(+)]. RNAi hypersensitive, effective RNAi in the nervous system. unc-119::GFP in neurons is almost completely suppressed on anti-GFP RNAi plates. Reduced progeny at 25C (almost sterile). nre-1(hd20) and lin-15B(hd126) seem very closely linked. Maintain at 15C or 20C. |
| VH715 |
hdIs17 I; hdIs10 V; nre-1(hd20) lin-15B(hd126) X. |
hdIs17 [glr-1::YFP + unc-47::YFP + unc-129::YFP + rol-6(su1006)]. hdIs10 [unc-129::CFP + glr-1::YFP + unc-47::DsRed + hsp-16::rol-6(su1006)]. Rollers. Reduced progeny at 25C (almost sterile). RNAi hypersensitive, effective RNAi in the nervous system. unc-47::DsRed is weak and only visible in adults. hsp-16::rol-6 transgene is not effectively Roll. Maintain at 15 or 20C. |
| XE1057 |
eri-1(mg366) IV; rde-1(ne219) V; lin-15B(n744) X. |
Reference: Firnhaber C & Hammarlund M. PLoS Genet. 2013 Nov;9(11):e1003921. |
| XE1375 |
wpIs36 I; wpSi1 II; eri-1(mg366) IV; rde-1(ne219) V; lin-15B(n744) X. |
wpIs36 [unc-47p::mCherry] I. wpSi1 [unc-47p::rde-1::SL2::sid-1 + Cbr-unc-119(+)] II. Maintain at 15-20C, sterile at 25C. GABAergic neuron-specific RNAi strain. Sensitivity to feeding RNAi is limited to the GABAergic neurons; all other tissues are resistant to RNAi. Superficially wild type with mCherry fluorescence in the GABA motor neurons. Reference: Firnhaber C & Hammarlund M. PLoS Genet. 2013 Nov;9(11):e1003921. |
| XE1474 |
wpSi6 II; eri-1(mg366) IV; rde-1(ne219) V; lin-15B(n744) X. |
wpSi6 [dat-1p::rde-1::SL2::sid-1 + Cbr-unc-119(+)] II. Maintain at 15-20C, sterile at 25C. Superficially wild-type. Dopaminergic neuron-specific RNAi strain. Sensitivity to feeding RNAi is limited to the dopaminergic neurons; all other tissues resistant. Reference: Firnhaber C & Hammarlund M. PLoS Genet. 2013 Nov;9(11):e1003921. |
| XE1581 |
wpSi10 II; eri-1(mg366) IV; rde-1(ne219) V; lin-15B(n744) X. |
wpSi10 [unc-17p::rde-1::SL2::sid-1 + Cbr-unc-119(+)] II. Maintain at 15-20C, sterile at 25C. Superficially wild-type. Cholinergic neuron-specific RNAi strain. Sensitivity to feeding RNAi is limited to the cholinergic neurons; all other tissues resistant. Reference: Firnhaber C & Hammarlund M. PLoS Genet. 2013 Nov;9(11):e1003921. |
| XE1582 |
wpSi11 II; eri-1(mg366) IV; rde-1(ne219) V; lin-15B(n744) X. |
wpSi11 [eat-4p::rde-1::SL2::sid-1 + Cbr-unc-119(+)] II. Maintain at 15-20C, sterile at 25C. Superficially wild-type. Glutamatergic neuron-specific RNAi strain. Sensitivity to feeding RNAi is limited to the glutamatergic neurons; all other tissues resistant. Reference: Firnhaber C & Hammarlund M. PLoS Genet. 2013 Nov;9(11):e1003921. |