| JJ1129 |
elt-1(zu180) unc-43(e408)/unc-24(e138) dpy-20(e1282) IV. |
Heterozygotes are WT and segregate WT, DpyUncs and dead eggs. |
| JJ938 |
unc-24(e138) daf-14(m77)/elt-1(zu180) dpy-20(e1282) IV. |
At 20C or 25C, heterozygotes are WT and segregate WT, UncDaf and dead eggs. At 15C, heterozygotes are WT and segregate WT, Uncs and dead eggs. |
| MH4799 |
elt-1(ku491) IV. |
Reference: Cohen ML, et al. PLoS Genet. 2015 Mar 27;11(3):e1005099. |
| MH4810 |
elt-1(ku491) IV; wIs51 V; daf-12(rh61rh411) X; kuEx194. |
wIs51 [SCMp::GFP + unc-119(+)] V. kuEx194 [elt-1(+) + sur-5p::DsRed]. GFP expression in seam cells. Pick DsRed+ animals to maintain. In a daf-12(WT) background, elt-1(ku491) exhibits some precocious fusion of seamcells and gaps in alae. elt-1(ku491); daf-12(rh61rh411) double mutants have more sever heterochronic phenotypes including seamcell proliferation and bursting vulvae. Reference: Cohen ML, et al. PLoS Genet. 2015 Mar 27;11(3):e1005099. |
| OH14408 |
elt-1(ok1002) IV; otEx6750. |
otEx6750 [unc-47p::mChopti + elt-1(+)(fosmid)]. Array rescues lethal elt-1 mutation; contains fosmid WRM0619bE05. Line 1-2. Reference: Gendrel M, et al. Elife. 2016 Oct 14;5. |
| OH14619 |
elt-1(ok1002) IV; him-5(e1490) V; otIs549 X; otEx6751. |
otIs549 [unc-25p::unc-25(partial)::mChopti::unc-54 3'UTR + pha-1(+)]. otEx6751 [unc-47p::GFP + elt-1(+)(fosmid)]. Him. otEx6751 rescues lethal elt-1 mutation; contains fosmid WRM0619bE05. otIs549 contains 1.8 kb upstream of the unc-25 start codon through exon 4; derived from injection of pMG154; line 2-1. Reference: Gendrel M, et al. Elife. 2016 Oct 14;5. |
| VC744 |
elt-1(ok1002) IV/nT1 [qIs51] (IV;V). |
W09C2.1. Homozygous lethal deletion chromosome balanced by GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1 aneuploids, and non-GFP ok1002 homozygotes (probable embryonic arrest). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807 |