| JH2874 |
pgl-1(ct131) him-3(e1147) III; ozIs5. |
ozIs5 [gld-1::GFP + unc-119(+)]. |
| JH2877 |
fbf-2(q738) II; pgl-1(ct131) him-3(e1147) III; axIs1722. |
axIs1722 [pie-1p::GFP::H2B::fog-1 3'UTR + unc-119(+)]. Maintain at 25C to maintain transgene expression. Unknown if ed3 is still in background. |
| JH2883 |
fbf-1(ok91) II; pgl-1(ct131) him-3(e1147) III; axIs1722. |
axIs1722 [pie-1p::GFP::H2B::fog-1 3'UTR + unc-119(+)]. Maintain at 25C to maintain transgene expression. Unknown if ed3 is still in background. |
| JH3269 |
pgl-1(ax3122[pgl-1::gfp]) IV. |
GFP inserted at C terminus of endogenous pgl-1 locus. Reference: Putnam A, et al. Nat Struct Mol Biol. 2019 Mar;26(3):220-226. |
| JK6022 |
pgl-1(q842) IV. |
pgl-1 (q842) is a (Q342A) missense mutation. This is a homozygous strain; replaced JK5481. Reference: Aoki ST, et al. Proc Natl Acad Sci U S A. 2016 Feb 2;113(5):1279-84. |
| SS2 |
pgl-1(ct131) him-3(e1147) IV. |
Temperature sensitive sterility. The sterility has both a maternal and a non-maternal component. Can be maintained indefinitely at low temperatures (16-23 C), with 7-19% sterile offspring. When low-temperature-grown homozygotes are allowed to produce progeny at 25C, the percentage of sterile offspring is 75-85%; at 26C the percentage of sterile offspring is 100%. Throws males. |
| SS579 |
pgl-1(bn101) IV. |
Temperature sensitive sterility. The sterility has both a maternal and a non-maternal component. Can be maintained indefinitely at low temperatures (16-23 C), with 7-19% sterile offspring. When low-temperature-grown homozygotes are allowed to produce progeny at 25C, the percentage of sterile offspring is 75-85%; at 26C the percentage of sterile offspring is 100%. |
| SS580 |
pgl-1(bn102) IV. |
Temperature sensitive sterility. The sterility has both a maternal and a non-maternal component. Can be maintained indefinitely at low temperatures (16-23 C), with 7-19% sterile offspring. When low-temperature-grown homozygotes are allowed to produce progeny at 25C, the percentage of sterile offspring is 75-85%; at 26C the percentage of sterile offspring is 100%. |
| SS615 |
pgl-1(bn101) unc-24(e138) IV; pgl-3(bn104) dpy-11(e224) V. |
Dpy Unc. Temperature sensitive. Maintain at 15C. Grows very slowly. |
| SS617 |
pgl-1(ct131) him-3(e1147) unc-24(e138) IV; pgl-3(bn104) dpy-11(e224) V. |
Throws males. Dpy. Unc. Mostly sterile at all temperatures, but a small fraction are fertile at low temperatures. Maintain at 15C. |
| SS618 |
pgl-1(ct131) him-3(e1147) IV; pgl-3(bn104) V. |
Throws males. Mostly sterile at all temperatures, but a small fraction are fertile at low temperatures. Maintain at 15C. |
| SS729 |
pgl-2(bn123) III; pgl-1(ct131) him-3(e1147) IV. |
Throws males. Maintain at 15C. Sterile at higher temperatures. |
| SS730 |
pgl-2(bn123) III; pgl-1(bn101) unc-24(e138) IV. |
Unc. Maintain at 15C. Sterile at higher temperatures. |
| SS733 |
pgl-2(bn123) III; pgl-1(bn101) unc-24(e138) IV; pgl-3(bn104) dpy-11(e224) V. |
Dpy. Unc. Mostly sterile at all temperatures, but a small fraction are fertile at low temperatures. Maintain at 15C. |
| YY1492 |
mut-16(cmp3[mut-16::gfp::flag + loxP] I; znfx-1(gg634[HA::tagRFP::znfx-1]) II; pgl-1(gg640[pgl-1::3xflag::mCardinal]) IV. |
gfp::flag inserted into endogenous mut-16 locus, 3xflag::gfp inserted into endogenous znfx-1 locus, and 3xflag::tagRFP inserted into endogenous pgl-1 locus using CRISPR/Cas9 engineering. Reference: Wan G, et al. Nature. 2018 May;557(7707):679-683. |
| YY968 |
znfx-1(gg544[3xflag::gfp::znfx-1]) II; pgl-1(gg547[pgl-1::3xflag::tagRFP]) IV. |
3xflag::gfp inserted into endogenous znfx-1 locus, and 3xflag::tagRFP inserted into endogenous pgl-1 locus using CRISPR/Cas9 engineering. Reference: Wan G, et al. Nature. 2018 May;557(7707):679-683. |