Gene Information: pgl-1

Namepgl-1 View on WormBase
Species C. elegans
SequenceZK381.4
Genetic positionIV:3.25 +/- 0.002 cM
Genomic positionIV: 6974441..6977318

Strains carrying this gene

Strain Genotype Description
JH2874 pgl-1(ct131) him-3(e1147) III; ozIs5. ozIs5 [gld-1::GFP + unc-119(+)].
JH2877 fbf-2(q738) II; pgl-1(ct131) him-3(e1147) III; axIs1722. axIs1722 [pie-1p::GFP::H2B::fog-1 3'UTR + unc-119(+)]. Maintain at 25C to maintain transgene expression. Unknown if ed3 is still in background.
JH2881 pgl-1(ct131) him-3(e1147) III; axIs1702. axIs1702 [GFP::fbf-2 + unc-119(+)]. Maintain at >22C to maintain transgene expression. Unknown if ed3 is still in background.
JH2883 fbf-1(ok91) II; pgl-1(ct131) him-3(e1147) III; axIs1722. axIs1722 [pie-1p::GFP::H2B::fog-1 3'UTR + unc-119(+)]. Maintain at 25C to maintain transgene expression. Unknown if ed3 is still in background.
JH3269 pgl-1(ax3122[pgl-1::gfp]) IV. GFP inserted at C terminus of endogenous pgl-1 locus. Reference: Putnam A, et al. Nat Struct Mol Biol. 2019 Mar;26(3):220-226.
JK6022 pgl-1(q842) IV. pgl-1 (q842) is a (Q342A) missense mutation. This is a homozygous strain; replaced JK5481. Reference: Aoki ST, et al. Proc Natl Acad Sci U S A. 2016 Feb 2;113(5):1279-84.
SS2 pgl-1(ct131) him-3(e1147) IV. Temperature sensitive sterility. The sterility has both a maternal and a non-maternal component. Can be maintained indefinitely at low temperatures (16-23 C), with 7-19% sterile offspring. When low-temperature-grown homozygotes are allowed to produce progeny at 25C, the percentage of sterile offspring is 75-85%; at 26C the percentage of sterile offspring is 100%. Throws males.
SS579 pgl-1(bn101) IV. Temperature sensitive sterility. The sterility has both a maternal and a non-maternal component. Can be maintained indefinitely at low temperatures (16-23 C), with 7-19% sterile offspring. When low-temperature-grown homozygotes are allowed to produce progeny at 25C, the percentage of sterile offspring is 75-85%; at 26C the percentage of sterile offspring is 100%.
SS580 pgl-1(bn102) IV. Temperature sensitive sterility. The sterility has both a maternal and a non-maternal component. Can be maintained indefinitely at low temperatures (16-23 C), with 7-19% sterile offspring. When low-temperature-grown homozygotes are allowed to produce progeny at 25C, the percentage of sterile offspring is 75-85%; at 26C the percentage of sterile offspring is 100%.
SS615 pgl-1(bn101) unc-24(e138) IV; pgl-3(bn104) dpy-11(e224) V. Dpy Unc. Temperature sensitive. Maintain at 15C. Grows very slowly.
SS617 pgl-1(ct131) him-3(e1147) unc-24(e138) IV; pgl-3(bn104) dpy-11(e224) V. Throws males. Dpy. Unc. Mostly sterile at all temperatures, but a small fraction are fertile at low temperatures. Maintain at 15C.
SS618 pgl-1(ct131) him-3(e1147) IV; pgl-3(bn104) V. Throws males. Mostly sterile at all temperatures, but a small fraction are fertile at low temperatures. Maintain at 15C.
SS729 pgl-2(bn123) III; pgl-1(ct131) him-3(e1147) IV. Throws males. Maintain at 15C. Sterile at higher temperatures.
SS730 pgl-2(bn123) III; pgl-1(bn101) unc-24(e138) IV. Unc. Maintain at 15C. Sterile at higher temperatures.
SS733 pgl-2(bn123) III; pgl-1(bn101) unc-24(e138) IV; pgl-3(bn104) dpy-11(e224) V. Dpy. Unc. Mostly sterile at all temperatures, but a small fraction are fertile at low temperatures. Maintain at 15C.
YY1492 mut-16(cmp3[mut-16::gfp::flag + loxP] I; znfx-1(gg634[HA::tagRFP::znfx-1]) II; pgl-1(gg640[pgl-1::3xflag::mCardinal]) IV. gfp::flag inserted into endogenous mut-16 locus, 3xflag::gfp inserted into endogenous znfx-1 locus, and 3xflag::tagRFP inserted into endogenous pgl-1 locus using CRISPR/Cas9 engineering. Reference: Wan G, et al. Nature. 2018 May;557(7707):679-683.
YY968 znfx-1(gg544[3xflag::gfp::znfx-1]) II; pgl-1(gg547[pgl-1::3xflag::tagRFP]) IV. 3xflag::gfp inserted into endogenous znfx-1 locus, and 3xflag::tagRFP inserted into endogenous pgl-1 locus using CRISPR/Cas9 engineering. Reference: Wan G, et al. Nature. 2018 May;557(7707):679-683.