| HS732 |
wrm-1(tm514) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). |
Homozygous lethal deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP tm514 homozygotes (probable embryonic or early larval arrest). Homozygous hT2[bli-4 let-? qIs48] inviable. Note: qIs48 has been observed to recombine off hT2, typically leaving behind a functional homozygous viable hT2 with Bli-4 phenotype. Pick WT GFP and check for correct segregation of progeny to maintain. |
| RW12263 |
wrm-1(st12263[wrm-1::TY1::EGFP::3xFLAG]) III. |
CRISPR/Cas9 engineered tagged endogneous locus. |
| VC519 |
wrm-1(ok738) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). |
B0336.1. Homozygous lethal deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ok738 homozygotes (probable embryonic or early larval arrest). Homozygous hT2[bli-4 let-? qIs48] inviable. Note: qIs48 has been observed to recombine off hT2, typically leaving behind a functional homozygous viable hT2 with Bli-4 phenotype. Pick WT GFP and check for correct segregation of progeny to maintain. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807 |
| WM74 |
wrm-1(ne1982) III. |
Temperature sensitive embryonic lethal. Maintain at 15C. Received new stock 3/20/2008 from Mello lab. |
| WM75 |
wrm-1(tm514) III; neIs2 IV. |
neIs2 [wrm-1::GFP + rol-6(su1006)] IV. Rollers. |