| HC114 |
ccIs4251 I; qtIs3 III; mIs11 IV; sid-1(qt9) V. |
ccIs4251 [(pSAK2) myo-3p::GFP::LacZ::NLS + (pSAK4) myo-3p::mitochondrial GFP + dpy-20(+)] I. qtIs3 [myo-2p::GFP dsRNA hairpin]. mIs11 [myo-2p::GFP + pes-10p::GFP + gut-promoter::GFP]. GFP expression in 4-cell embryos, pharyngeal muscle and gut. Resistant to systemic RNAi by feeding and injection and endogenous hairpin expression. |
| HC196 |
sid-1(qt9) V. |
Null allele. Systemic RNAi defective. Reference: Jose AM, et al. Proc Natl Acad Sci U S A. 106(7):2283-8. |
| HC726 |
sid-1(qt95) V. |
Sid. Hypomorhic allele. Reference: Whangbo JS, et al. G3 (Bethesda). 2017 Oct 12 [Epub ahead of print]. pii: g3.300308.2017. doi: 10.1534/g3.117.300308. PMID: 29025917. |
| HC75 |
ccIs4251 I; sid-1(qt2) V. |
ccIs4251 [(pSAK2) myo-3p::GFP::LacZ::NLS + (pSAK4) myo-3p::mitochondrial GFP + dpy-20(+)] I. Resistant to systemic RNAi, but sensitive to autonomous RNAi. |
| HC970 |
sid-1(qt78) V. |
Sid. Deletion allele. Reference: Whangbo JS, et al. G3 (Bethesda). 2017 Oct 12 [Epub ahead of print]. pii: g3.300308.2017. doi: 10.1534/g3.117.300308. PMID: 29025917. |
| MGH171 |
sid-1(qt9) V; alxIs9. |
alxIs9 [vha-6p::sid-1::SL2::GFP]. Gut-sensitive RNAi line. Stable line with strong GFP signal. |
| NL3321 |
sid-1(pk3321) V. |
Resistant to feeding dsRNA but sensitive to dsRNA injection. PKA rsd-8. |
| TU3403 |
ccIs4251 I; sid-1(qt2) V; uIs71. |
ccIs4251 [(pSAK2) myo-3p::GFP::LacZ::NLS + (pSAK4) myo-3p::mitochondrial GFP + dpy-20(+)] I. uIs71 [(pCFJ90) myo-2p::mCherry + mec-18p::sid-1]. TRN-specific feeding RNAi. Reference: Calixto et al. (2010) Nature Methods 7:554-9. |
| TU3568 |
sid-1(pk3321) him-5(e1490) V; lin-15B(n744) X; uIs71. |
uIs71 [(pCFJ90) myo-2p::mCherry + mec-18p::sid-1]. TRN-specific RNAi by feeding. Him (~50% males). Maintain 15-20 degrees. Reference: Calixto et al. (2010) Nature Methods 7:554-9. |
| TU3595 |
sid-1(pk3321) him-5(e1490) V; lin-15B(n744) X; uIs72. |
uIs72 [pCFJ90(myo-2p::mCherry) + unc-119p::sid-1 + mec-18p::mec-18::GFP]. Hypersensitive neuronal RNAi by feeding. GFP detectable in TRNs. Him (~50% males). Maintain 15-20 degrees. Reference: Chalfie (2010) Worm Breeders Gazette. |
| TU3596 |
sid-1(pk3321) him-5(e1490) V; lin-15B(n744) X. |
Him. Enhanced RNAi background. Maintain under normal conditions. |