Gene Information: sid-1

Namesid-1 View on WormBase
Species C. elegans
SequenceC04F5.1
Genetic positionV:-2.49 +/- 0.005 cM
Genomic positionV: 5119970..5126916

Strains carrying this gene

Strain Genotype Description
HC114 ccIs4251 I; qtIs3 III; mIs11 IV; sid-1(qt9) V. ccIs4251 [(pSAK2) myo-3p::GFP::LacZ::NLS + (pSAK4) myo-3p::mitochondrial GFP + dpy-20(+)] I. qtIs3 [myo-2p::GFP dsRNA hairpin]. mIs11 [myo-2p::GFP + pes-10p::GFP + gut-promoter::GFP]. GFP expression in 4-cell embryos, pharyngeal muscle and gut. Resistant to systemic RNAi by feeding and injection and endogenous hairpin expression.
HC196 sid-1(qt9) V. Null allele. Systemic RNAi defective. Reference: Jose AM, et al. Proc Natl Acad Sci U S A. 106(7):2283-8.
HC726 sid-1(qt95) V. Sid. Hypomorhic allele. Reference: Whangbo JS, et al. G3 (Bethesda). 2017 Oct 12 [Epub ahead of print]. pii: g3.300308.2017. doi: 10.1534/g3.117.300308. PMID: 29025917.
HC75 ccIs4251 I; sid-1(qt2) V. ccIs4251 [(pSAK2) myo-3p::GFP::LacZ::NLS + (pSAK4) myo-3p::mitochondrial GFP + dpy-20(+)] I. Resistant to systemic RNAi, but sensitive to autonomous RNAi.
HC970 sid-1(qt78) V. Sid. Deletion allele. Reference: Whangbo JS, et al. G3 (Bethesda). 2017 Oct 12 [Epub ahead of print]. pii: g3.300308.2017. doi: 10.1534/g3.117.300308. PMID: 29025917.
MGH171 sid-1(qt9) V; alxIs9. alxIs9 [vha-6p::sid-1::SL2::GFP]. Gut-sensitive RNAi line. Stable line with strong GFP signal.
NL3321 sid-1(pk3321) V. Resistant to feeding dsRNA but sensitive to dsRNA injection. PKA rsd-8.
TU3401 sid-1(pk3321) V; uIs69 V. uIs69 [pCFJ90 (myo-2p::mCherry) + unc-119p::sid-1]. Hypersensitive neuronal RNAi by feeding. Superficially wild-type. Maintain 15-20 degrees. [NOTE: uIs69 is closely linked and maps to the right of dpy-11. (C. Loer 08/2011) See strains LC105 and LC108.] Reference: Calixto et al. (2010) Nature Methods 7:554-9.
TU3403 ccIs4251 I; sid-1(qt2) V; uIs71. ccIs4251 [(pSAK2) myo-3p::GFP::LacZ::NLS + (pSAK4) myo-3p::mitochondrial GFP + dpy-20(+)] I. uIs71 [(pCFJ90) myo-2p::mCherry + mec-18p::sid-1]. TRN-specific feeding RNAi. Reference: Calixto et al. (2010) Nature Methods 7:554-9.
TU3568 sid-1(pk3321) him-5(e1490) V; lin-15B(n744) X; uIs71. uIs71 [(pCFJ90) myo-2p::mCherry + mec-18p::sid-1]. TRN-specific RNAi by feeding. Him (~50% males). Maintain 15-20 degrees. Reference: Calixto et al. (2010) Nature Methods 7:554-9.
TU3595 sid-1(pk3321) him-5(e1490) V; lin-15B(n744) X; uIs72. uIs72 [pCFJ90(myo-2p::mCherry) + unc-119p::sid-1 + mec-18p::mec-18::GFP]. Hypersensitive neuronal RNAi by feeding. GFP detectable in TRNs. Him (~50% males). Maintain 15-20 degrees. Reference: Chalfie (2010) Worm Breeders Gazette.
TU3596 sid-1(pk3321) him-5(e1490) V; lin-15B(n744) X. Him. Enhanced RNAi background. Maintain under normal conditions.