| AWR41 |
lin-35(kea7[lin-35p::degron::GFP::lin-35]) I. |
N-terminal degron::GFP tag was inserted into the endogenous lin-35 locus. Generated in N2 background. Reference: Willis AR, et al. Sci Adv. 2021 May 5;7(19):eabf3114. PMID: 33952520 [NOTE: lin-35(kea7) is an N-terminal tag; the methods section of the paper incorrectly describes the tag as a C-terminal insertion.] |
| AWR54 |
lin-35(kea7[lin-35p::degron::GFP::lin-35]) I; ieSi57 II. |
ieSi57 [eft-3p::TIR1::mRuby::unc-54 3’UTR + Cbr-unc-119(+)] II. N-terminal degron::GFP tag was inserted into the endogenous lin-35 locus. A single copy insertion of eft-3p::TIR1::mRuby allows auxin-inducible degradation of LIN-35 in somatic tissues. Generated in N2 background. Reference: Willis AR, et al. Sci Adv. 2021 May 5;7(19):eabf3114. PMID: 33952520 [NOTE: lin-35(kea7) is an N-terminal tag; the methods section of the paper incorrectly describes the tag as a C-terminal insertion.] |
| AWR56 |
lin-35(kea7[lin-35p::degron::GFP::lin-35]) I; ieSi64 II. |
ieSi64 [gld-1p::TIR1::mRuby::unc-54 3’UTR + Cbr-unc-119(+)] II. N-terminal degron::GFP tag was inserted into the endogenous lin-35 locus. A single copy insertion of gld-1p::TIR1::mRuby allows for auxin inducible degradation of LIN-35 in the germline. Generated in N2 background. Reference: Willis AR, et al. Sci Adv. 2021 May 5;7(19):eabf3114. PMID: 33952520 [NOTE: lin-35(kea7) is an N-terminal tag; the methods section of the paper incorrectly describes the tag as a C-terminal insertion.] |
| AWR58 |
lin-35(kea7[lin-35p::degron::GFP::lin-35]) I; keaSi10 II. |
keaSi10 [rpl-28p::TIR1::mRuby::unc-54 3’UTR + Cbr-unc-119(+)] II. N-terminal degron::GFP tag was inserted into the endogenous lin-35 locus. A single copy insertion of rpl-28::TIR1::mRuby allows auxin-inducible degradation of LIN-35 throughout the animal. Generated in N2 background. Reference: Willis AR, et al. Sci Adv. 2021 May 5;7(19):eabf3114. PMID: 33952520 [NOTE: lin-35(kea7) is an N-terminal tag; the methods section of the paper incorrectly describes the tag as a C-terminal insertion.] |
| GR1379 |
lin-35(n745) I; eri-1(mg366) IV. |
Temperature sensitive sterile at 25C. Maintain at 15C. |
| LG331 |
lin-35(n745) I; geIs7. |
geIs7 [skn-1b::GFP]. Reference: Nature (2007) 447(7144):545-9. |
| MR164 |
lin-35(rr33) I; rrIs1. |
rrIs1 [elt-2::GFP + unc-119(+)]. Extra intestinal nuclei. Superficially wild-type at 20C. Lethal at 25C. |
| MT10430 |
lin-35(n745) I. |
synMuv with n111. |
| MT15488 |
lin-35(n4760) I. |
Class B SynMuv. Reduced fertility. Maintain at 20C or cooler. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. |
| MT1624 |
lin-35(n745) I; lin-8(n111) II. |
Double mutant is Muv. lin-35 alone is non-Muv. lin-35 is a class B synthetic Muv. |
| MT3033 |
unc-13(e1091) lin-35(n745) I. |
Unc. |