EEG107 |
tph-1(mg280) II; mudIs1. |
mudIs1 [tph-1p::ChR2::GFP + myo-3p::mCherry]. When tph-1 mutants carrying mudIs1 are exposed to blue light, the worms continue to move rather than stopping like wild-type animals. Reference: Pokala N & Glater EE. 2018. Journal of Undergraduate Neuroscience Education. 162(2): A152-A158. |
GR1321 |
tph-1(mg280) cam-1(vs166) II. |
Slow pumping. Egg laying defective. Low frequency of dauer formation. Residual serotonin immunoreactivity, rare and very faint (<1% of NSMs) in most stages, but nearly 100% of CP neurons in adult males show faint to moderate serotonin immunoreactivity. Males mate quite well (E. Hare and C. Loer). Some phenotypic defects originally attributed to mg280 in this strain are likely due to vs166. vs166 is a large deletion (approx. 9.8kb) in the cam-1 gene; flanking sequences are: 5'-gctactggtaaataaggtaa-3' and 5'-atgcttttaaagtttatatt-3' (Edith Myers, personal commnication). See MT15434 for a strain carrying mg280 without the cam-1 mutation. |
GR1333 |
yzIs71 V. |
yzIs71 [tph-1p::GFP + rol-6(su1006)] V. Rollers. tph-1 transcriptional reporter containing 3.1kb of tph-1 5’ regulatory sequence through the beginning of exon 4. Reference: Lloret-Fernández et al. eLife 2018;7:e32785 DOI: 10.7554/eLife.32785. Sze JY, et al. Nature. 2000 Feb 3;403(6769):560-4. |
GR1366 |
mgIs42. |
mgIs42 [tph-1::GFP + rol-6(su1006)]. Rollers. |
MT14984 |
tph-1(n4622) II. |
Egl. Reduced pharyngeal pumping. |
MT15434 |
tph-1(mg280) II. |
Backcrossed tph-1(mg280) allele. cam-1 mutation was removed by crossing left and right of tph-1(mg280) using bli-2 and unc-4. Strain does not have withered tail defect and moves well. |
OH4127 |
zdIs13 IV; oyIs14 V; wrk-1(ok695) X. |
zdIs13 [tph-1p::GFP] IV. oyIs14 [sra-6::GFP + lin-15(+)] V. |
OH4134 |
otIs173 III; zdIs13 IV. |
otIs173[F25B3.3::DsRed2 + ttx-3promB::GFP] III. zdIs13 [tph-1p::GFP] IV. |
OH4135 |
otIs173 III; zdIs13 IV; wrk-1(ok695) X. |
otIs173[F25B3.3::DsRed2 + ttx-3promB::GFP]. zdIs13 [tph-1p::GFP] IV. |
OH4138 |
zdIs13 IV; wrk-1(tm1099) X. |
zdIs13 [tph-1p::GFP] IV. |
OH4143 |
vab-1(dx31) II; zdIs13 IV. |
zdIs13 [tph-1p::GFP] IV. |
OH4144 |
vab-1(dx31) II; zdIs13 IV; wrk-1(ok695) X. |
zdIs13 [tph-1p::GFP] IV. |
OH4147 |
zdIs13 IV; wrk-1(ok695) X. |
zdIs13 [tph-1p::GFP] IV. |