| ATD6 |
par-6(zu222) unc-101(m1)/hIn1[unc-54(h1040)] I; unc-119(ed3) III; zuIs45 V. |
zuIs45 [nmy-2p::nmy-2::GFP + unc-119(+)] V. Balanced worms are wild-type and segregate wild-type (heterozygotes), Coil Par (par-6 unc-101 homozygotes; maternal effect lethal), and paralyzed Unc (hIn1 homozygotes). Par phenotype is slightly leaky, but survivors are agametic. NMY-2::GFP is expressed in the germline and somatic gonad. Cross of JJ1473 and KK818. Unknown if unc-119(ed3) is still present or homozygous in background. Reference: Small LE & Dawes AT. Mol Biol Cell. 2017 Aug 1;28(16):2220-2231. |
| FT36 |
unc-101(m1) par-6(zu170) I; zuIs43. |
zuIs43[pie-1::GFP::PAR-6::ZF1 + unc-119(+)]. Unc worms. GFP present in early embryos but then degrades in somatic lineages. Rescues Mel phenotype of par-6(zu170). |
| JJ1578 |
par-6(zu222) unc-101(m1); zuIs54. |
zuIs54 [par-6p::par-6::ZF1::GFP + unc-119(+)]. Unc. GFP-tagged PAR-6 that degrades in early embryonic somatic cells; rescues the Par phenotype of par-6(zu222). Delayed gastrulation of endodermal cells. Reference: Nance J, Munro EM, Priess JR. Development. 2003 Nov;130(22):5339-50. |
| JJ1743 |
par-6(tm1425)/hIn1 [unc-54(h1040)] I; him-8(e1489) IV. |
Heterozygotes are WT and segregate WT, Uncs, dead larvae (par-6 homozygotes) and males. |
| KK1248 |
par-6(it310[par-6::GFP]) I. |
Superficially wild-type. Made in N2 background. [NOTE: There was an error in the information originally submitted to the CGC for this strain. The correct allele name is par-6(it310), not par-6(it319).] |
| KK818 |
par-6(zu222) unc-101(m1)/hIn1 [unc-54(h1040)] I. |
Heterozygotes are WT and segregate WT, paralyzed Unc, and Coilers which give only dead eggs (slightly leaky, but survivors are agametic). zu222 is a strict maternal effect embryonic lethal. Partitioning defect similar to that of par-3; fails to localize PAR-3 protein. |
| LP216 |
par-6(cp45[par-6::mNeonGreen::3xFlag + LoxP unc-119(+) LoxP]) I; unc-119(ed3) III. |
PAR-6::mNG endogenously tagged using Cas9-triggered homologous recombination. Reference: Dickinson DJ, et al. Dev Cell. 2017 Aug 21;42(4):416-434.e11. (PMID 28829947). |
| LP244 |
par-6(cp60[par-6::mKate2::3xMyc + LoxP unc-119(+) LoxP]) I; unc-119(ed3) III. |
PAR-6::mKate2 endogenously tagged using Cas9-triggered homologous recombination. Reference: Dickinson DJ, et al. Dev Cell. 2017 Aug 21;42(4):416-434.e11. (PMID 28829947). |
| LP653 |
par-6(cp345[par-6::HaloTag-GLO]) I. |
PAR-6::Halo endogenously tagged using Cas9-triggered homologous recombination. HaloTag-GLO is a germline-optimized variant coded to be less prone to silencing in the germline. Reference: Dickinson DJ, et al. Dev Cell. 2017 Aug 21;42(4):416-434.e11. (PMID 28829947). |