ATD3 |
lon-1(e185) par-3(e2074) unc-119(ed3) III; zuIs45 V; sDp3(III;f) |
zuIs45 [nmy-2p::nmy-2::GFP + unc-119(+)] V. Pick wild-type to maintain. Worms carrying the sDp3 duplication are wild-type; animals that have lost the duplication are Lon Par (maternal effect lethal). Cross of JJ1473 and KK237. Unknown if unc-119(ed3) is still present or homozygous in background. Reference: Small LE & Dawes AT. Mol Biol Cell. 2017 Aug 1;28(16):2220-2231. |
EU918 |
lon-1(e185) par-3(it71)/qC1 [dpy-19(e1259) glp-1(q339)] III; spn-4(or191) V. |
Temperature sensitive spn-1. Maintain at 15C. Lethal at 25C. Heterozygotes are WT and segregate WT, Lon which give only dead eggs at all temperatures, and Sterile Dpys(ts). |
JH2756 |
ect-2(ax751) II; par-3(it71) unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] III; zuIs45 V. |
zuIs45 [nmy-2::NMY-2::GFP + unc-119(+)] V. Temperature-sensitive. Maternal-effect lethal at 25 C. Maintain at 15-20 C. Segregates Unc Mel, non-Unc Mel, non-Unc Ste. Reference: Zonies S, et al. Development. 2010 May;137(10):1669-77. |
JJ1600 |
par-3(it71) lon-1(e185) III; him-8(e1489) IV; zuIs20. |
zuIs20 [par-3p::par-3::ZF1::GFP + unc-119(+)]. Lon. Him. GFP-tagged PAR-3 that is degraded in early embryonic somatic cells. Rescues the Par phenotype of par-3(it71). Delayed endodermal cell gastrulation. Reference: Nance J, Munro EM, Priess JR. Development. 2003 Nov;130(22):5339-50. |
KK1216 |
par-3(it298[par-3::GFP]) III. |
Superficially wild-type. Made in N2 background. |
KK1218 |
par-3(it300[par-3::mCherry]) III. |
Superficially wild-type. Made in N2 background. |
KK237 |
lon-1(e185) par-3(e2074) III; sDp3 (III;f). |
WT sDp3-bearing animals segregate WT and lon-1 par-3 homozygotes that lay eggs that don't hatch. par-3 exhibits mildly ts penetrance in homozygotes. Strict maternal effect. Received new stock 3/01. |
KK241 |
lon-1(e185) par-3(it62) III; sDp3 (III;f). |
Animals with the Duplication are WT. Animals which have lost the Duplication are Lon and throw dead eggs. |
KK571 |
lon-1(e185) par-3(it71)/qC1 [dpy-19(e1259) glp-1(q339)] III. |
Heterozygotes are WT and segregate WT, Dpy Steriles, and Lon which give only dead eggs. |
LP242 |
par-3(cp54[mNeonGreen::3xFlag::par-3]) III. |
mNG::PAR-3 endogenously tagged using Cas9-triggered homologous recombination. Reference: Dickinson DJ, et al. Dev Cell. 2017 Aug 21;42(4):416-434.e11. (PMID 28829947). |
LP621 |
par-3(cp323[HaloTag-GLO^PAR-3]) III. |
Halo::PAR-3 endogenously tagged using Cas9-triggered homologous recombination. HaloTag-GLO is a germline-optimized variant coded to be less prone to silencing in the germline. Reference: Dickinson DJ, et al. Dev Cell. 2017 Aug 21;42(4):416-434.e11. (PMID 28829947). |