Gene Information: sma-4

Namesma-4 View on WormBase
Species C. elegans
SequenceR12B2.1
Genetic positionIII:-1.44 +/- 0.001 cM
Genomic positionIII: 5816232..5820204

Strains carrying this gene

Strain Genotype Description
DR1369 sma-4(e729) III. Short, non-Dpy. Males have crumpled spicules.
FK412 sma-4(e729) III; sma-5(n678) X. Very small. Small brood size (about 1/10 that of WT). Grows very slowly. Line A. Reference: Watanabe, N et al. Genes to Cells 2007. 12(5):603-609.
FK413 sma-4(e729) III; sma-5(n678) X. Very small. Small brood size (about 1/10 that of WT). Grows very slowly. Line C. Reference: Watanabe, N et al. Genes to Cells 2007. 12(5):603-609.
KK204 sma-4(e729) mab-5(e1239) unc-36(e251) III. Small and Unc.
LW5558 sma-4(jj278) III. sma-4(jj278) worms are small, and the mutation can suppress the sma-9(0) loss of M-derived coelomocyte defect. sma-4(jj278) is a true molecular null allele of sma-4; the 3,556 bp deletion (position: Chromosome III: 5,816,203….5,819,759) removes almost the entire coding region of sma-4. Reference: McKillop AN, et al. (2018). A new deletion allele of sma-4. microPublication Biology. https://doi.org/10.17912/Z4Z9-CE10.
PJ1227 sma-4(e729) III; ccIs55 V. ccIs55 [unc-54::lacZ + sup-7(st5)] V. Small body size. Slow growth. Strong lacZ staining in body wall.
VC2410 sma-4(ok3140) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). R12B2.1. Homozygous lethal deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ok3140 homozygotes (late larval arrest). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: GACGGAAAGGTGTTCCACAT. External right primer: GGTCCGTGCAGAAAATCAGT. Internal left primer: CGCAAGAATATGGAGATGGC. Internal right primer: TGCTCGTACTGCTTCATTGC. Internal WT amplicon: 1288 bp. Deletion size: 718 bp. Deletion left flank: AGAGGTGGCTGCTCTCTCTCTCTGACTTTT. Deletion right flank: TTCGTCCGATCCGGGTACCTAGACTACACT. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807