| DR411 |
dpy-13(e184)/daf-15(m81) IV. |
Heterozygotes are semi-Dpy and segregate semi-Dpy, Dpy and dauers. Dauers are lethal and SDS-sensitive. NOTE: WT recombinants that have lost dpy-13 can quickly overtake a population. |
| DR412 |
daf-15(m81)/unc-24(e138) IV. |
Heterozygotes are WT and segregate WT, Unc, and lethal dauers. Dauers are SDS-sensitive. |
| DR732 |
daf-15(m81) unc-22(s7)/nT1 IV; +/nT1 V. |
Heterozygotes are WT and segregate WT, Vul, Twitchers which are dauers (dauer constitutive) and dead eggs. Maintain by picking WT. Hets twitch in 1% nicotine. |
| DV3208 |
daf-15(re147[daf-15::mNG::2xHA]) IV. |
mNeonGreen tag inserted at 3' end of endogenous daf-15 locus. Ubiquitous expression. |
| DV3525 |
daf-15(re257[daf-15::mNG::AID]) IV. |
mNeonGreen tag inserted at 3' end of endogenous daf-15 locus; AID at 3' end of mNeonGreen. Ubiquitous expression. Transgene can be degraded in a background expressing TIR1 co-factor and supplemented with auxin. |
| HR83 |
mel-24(ct59) dpy-20(e1282)/unc-24(e138) daf-15(m81) IV. |
Heterozygotes are WT and segregate WT, Unc Dauers, and Dpys which throw dead eggs. Maintain by picking WT. ct59 animals are viable and fertile at 15C. ct59 is a temperature sensitive dominant maternal effect lethal. ct59 hets give more viable offspring at 15C than 25C. |
| VC1027 |
daf-15(ok1412)/nT1 IV; +/nT1 V. |
C10C5.6a. Homozygous lethal deletion chromosome balanced by translocation. Heterozygotes are WT and segregate WT, arrested nT1 aneuploids, vulvaless nT1 homozygotes, and ok1412 homozygotes (arrested incomplete dauers). Pick WT and check for correct segregation of progeny to maintain. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807 |