Gene Information: ced-3

Nameced-3 View on WormBase
Species C. elegans
SequenceC48D1.2
Genetic positionIV:8.47 +/- 0.004 cM
Genomic positionIV: 13198261..13204137

Strains carrying this gene

Strain Genotype Description
DA2356 ced-1(e1735) I; ced-3(n717) IV; lin-15B&lin-15A(n765) X; adEx2342. adEx2342 [efl-3::mCherry::FLAG + LIN-15(+)]. Maintain by picking non-Muv. Fluorescence is dim, but array is stable (>95% transmission).
DL308 ced-3(n717) IV; mxIs28 X. mxIs28 [ceh-20p::ceh-20::YFP + lin-15(+)] X. Reference: Potts MB, Wang DP, & Cameron S. Dev Biol. 2009 May 15;329(2):374-85.
KX84 ced-3(n2452) IV; bcIs39 V. bcIs39 [lim-7p::ced-1::GFP and lin-15(+)] V.  Resistant to germ cell apoptosis. No apoptosing germ cells are decorated by CED-1::GFP in the gonad. ced-3 deletion confirmed by genomic triple primer PCR with 5’-AGTTCACCGTGACAGCGTCTCTTC-3’, 5’-CGATTACGACTTGAACTGTATCCGA-3’, and 5’-TCTTGTGTAAACGAGATTTGCAATG-3’. Homozygous ced-3(n2452) yields only 1,110 bp product. Outcrossed heterozygotes yield both 1,411 bp (wild type ced-3) and 1,110 bp products. [NOTE: (11-05-2019) A user has reported this strain exhibits temperature-sensitive sterility when raised at 25C.]
MT1522 ced-3(n717) IV. Abnormal cell death. Cells that normally die survive.
MT1743 ced-3(n718) IV. n718 is a strong allele of ced-3.
MT2405 ced-3(n717) unc-26(e205) IV. Unc. Abnormal cell death. Cells that normally die survive.
MT2563 ced-3(n1165) IV; egl-1(n487) V. n1165 blocks cell death. Temperature sensitive Egl. Semi-dominant Egl.
MT3002 ced-3(n1286) IV. Absence of cell death. Strong allele.
MT5166 ced-3(n1040) IV.
MT5353 ced-3(n1949) IV. Strong allele of ced-9. Reference: (1999) Genetics 153(4):1655-71.
MT5729 dpy-20(e1282) unc-30(e191) ced-3(n717) IV. Dpy (ts). Unc. Abnormal cell death. Cells that normally die survive.
MT5811 ced-1(e1735) I; ced-3(n717) IV.
MT6347 ced-3(n2433) IV. n2433 is a suppressor of ced-9(n1950n2161).
MT7169 sem-4(n1378) I; ced-5(n2098) ced-3(n2720) IV.
MT7170 sem-4(n1378) I; ced-5(n2098) ced-3(n2719) IV.
MT7386 ced-9(n2812) III; ced-3(n717) IV. n2812 is a strong loss-of-function allele and a maternal effect lethal.
MT7502 sem-4(n1378) I; ced-5(n2098) ced-3(n2830) IV.
MT7632 ced-3(n2432) IV. Suppressor of ced-9(n1950 n2162). Reference: (1999) Genetics 153(4):1655-71.
MT7735 ced-9(n2812) III; ced-3(n2427) IV.
MT8309 ced-3(n2921) IV. Suppressor of ced-9(n1950n2161) maternal effect lethality.
MT8312 ced-3(n2877) IV. Medium strong allele. Suppressor of ced-9(n1950n2161) maternal effect lethality.
MT8313 ced-3(n2885) IV. Medium-strong alllele; suppresses of ced-9(n1950 n2162) maternal effect lethality. Reference: (1999) Genetics 153(4):1655-71.
MT8319 ced-3(n2888) IV. Suppressor of ced-9(n1950n2161) maternal effect lethality. Strong allele of ced-3.
MT8347 ced-3(n2452) IV.
MT8352 ced-3(n2439) IV. Suppressor of ced-9(n1950n2161) maternal effect lethality.
MT8354 ced-3(n2454) IV. n2454 is a strong allele of ced-3.
MT8696 ced-3(n2449) IV. n2449 is a weak allele of ced-3.
MT8699 ced-3(n2424) IV. n2424 is a weak allele of ced-3.
OH4322 ced-3(ot289) IV; vtIs1 V. vtIs1 [dat-1p::GFP + rol-6(su1006)] V. Rollers. The undead sisters of CEPvs and PVDs express dat-1::GFP.
RB2071 ced-3(ok2734) IV. C48D1.2. Homozygous. Outer Left Sequence: CAAAAGGACGCTCTGCCTAT. Outer Right Sequence: TGTCGTGTCGAGACCAGGTA. Inner Left Sequence: AGCAGATCGATTGTTGTTCAAG. Inner Right Sequence: TTGGTCCCAAAAACCAAAAA. Inner Primer PCR Length: 1118 bp. Deletion Size: 682 bp. Deletion left flank: GGCTTTCGCTCCAAAGAATATAAAATAATC. Deletion right flank: TTAAATATCAGTTTACATTAGGAACAAGGC. Insertion Sequence: TT. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
XR2 ced-3(n717) IV; abl-1(ok171) X.
XR5 lagr-1(gk327) I; hyl-1(ok976) IV; ced-3(n717) IV.