Gene Information: egl-27

Nameegl-27 View on WormBase
Species C. elegans
SequenceC04A2.3
Genetic positionII:0.12 +/- 0.001 cM
Genomic positionII: 6835008..6847908

Strains carrying this gene

Strain Genotype Description
CF1192 egl-27(n170) II; muIs35 V. muIs35 [mec-7::GFP + lin-15(+)].
JA1194 egl-27(we3) II. egl-27(we3) animals have variable body morphology defects with cold sensitive embryonic/L1 lethality (5% die at 22C, 90% at 15C). Maternal effect vab-7(ed6) enhancer; semi-lethal with vab-7(ed6).
KS100 egl-27(ok151)/mnC1 [dpy-10(e128) unc-52(e444)] II. Heterozygotes are WT and segregate WT, Egl and DpyUnc. Temperature sensitive Egl. Do not grow at 15C.
KS99 egl-27(ok151) II. Cold sensitive - do not grow at 15C. Egl.
MT170 egl-27(n170) II. Egg laying defective. Retains late stage eggs. Males do not mate.
SD1625 egl-27(we3) II; daf-2(e1370) III. Maintain at 20C. Embryonic lethal at 15C. Dauer formation at 25C. Reference: Xu X, Kim SK. PLoS Genet. 2012;8(12):e1003108.
SD1743 egl-27(we3) II; daf-2(e1370) III; muIs84. muIs84 [sod-3p::GFP + rol-6(su1006)]. Maintain at 20C. Embryonic lethal at 15C. Dauer formation at 25C. GFP should be visible at low magnification. Reference: Xu X, Kim SK. PLoS Genet. 2012;8(12):e1003108.
SD1746 egl-27(we3) II; muIs84. muIs84 [sod-3p::GFP + rol-6(su1006)]. Maintain at 20C or higher. Embryonic lethal at 15C. GFP should be visible at low magnification. Reference: Xu X, Kim SK. PLoS Genet. 2012;8(12):e1003108.
SD1751 egl-27(we3) II; wgIs177. wgIs177 [egl-27p::TY1::EGFP::3xFLAG + unc-119(+)]. Maintain at 20C or higher. Embryonic lethal at 15C. Reference: Xu X, Kim SK. PLoS Genet. 2012;8(12):e1003108.
SD1862 egl-27(we3) II; ccIs4251; stIs10161. ccIs4251 [(pSAK2) myo-3p::GFP::LacZ::NLS + (pSAK4) myo-3p::mitochondrial GFP + dpy-20(+)] I. stIs10161 [egl-27p::HIS-24::mCherry + unc-119(+)]. Maintain at 20C or higher. Embryonic lethal at 15C. mCherry should be visible at low magnification. Reference: Xu X, Kim SK. PLoS Genet. 2012;8(12):e1003108.
VC1217 egl-27(ok1670) II. C04A2.3. External left primer: TCGAGTTGGGCTCAGTCTTT. External right primer: CAGCGATGATGATGAAGGAA. Internal left primer: GGTAAAAGCTGCCAATCCAA. Internal right primer: CTTGTCCTCACTCCGCTCTC. Internal WT amplicon: 2523 bp. Deletion size: 1747 bp. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807