CF439 |
lin-39(mu26) III; dpy-20(e1282) IV; him-5(e1490) V; muIs23. |
muIs23 [hsp::lin-39 + (pMH86) dpy-20(+)]. Heat-shock inducible lin-39. muIs23 is a spontaneous integrant whose chromosomal location is unknown. [NOTE: This strain was previously described as carrying lin-39(n1760), but it is actually carrying the g to a substitution of mu26. Strain MT7255 has been confirmed to be carrying the a to t substitution of n1760.] |
KRA102 |
lin-39(kas1) III; ynIs40 V; unc-3(n3435) X. |
ynIs40 [flp-11p::GFP] V. kas1 is a point mutation in the second intron splice acceptor site, disrupting splicing. Worms are vulvaless and have severe locomotion defects. Reference: Feng W, et al. Elife. 2020 Jan 3;9. pii: e50065. doi: 10.7554/eLife.50065. |
KRA467 |
lin-39(kas9[lin-39::mNG::AID]) III. |
mNeonGreen::3xFLAG::AID was inserted at the C-terminus of the endogenous lin-39 locus by CRISPR. Endogenous lin-39 expression marked by mNG. LIN-39 protein can be degraded by Auxin application with expression of TIR-1 protein. Reference: Feng W, et al. Elife. 2020 Jan 3;9. pii: e50065. doi: 10.7554/eLife.50065. |
MT1514 |
lin-39(n709) III. |
Temperature sensitive Vul. 12% Vul at 15C and 45% Vul at 25C. VC's die. Variably Egl. Variably abnormal vulval divisions. See also WBPaper00001768 |
MT4007 |
lin-39(n1760) III. |
Vulvaless. Strong loss of function allele. |
MT4009 |
lin-39(n1760)/dpy-17(e164) unc-32(e189) III. |
Heterozygotes are WT and segregate WT, DpyUnc and Vul. Pick WT to maintain. |
MT4306 |
lin-39(n1490) unc-36(e251) III. |
Unc. Vulvaless (temperature sensitive-91% Vul at 15C and 100% Vul at 20C and 25C). n1490 is also temperature sensitive Unc. |
MT4491 |
lin-39(n1872) III. |
Vulvaless. Temperature sensitive. Suppressor of n765. |
MT4498 |
lin-39(n1880) III. |
Vulvaless. Strong loss of function allele. |
MT4500 |
lin-39(n1880)/dpy-17(e164) unc-32(e189) III. |
Heterozygotes are WT and segregate WT, DpyUnc and Vul. Pick WT to maintain. |
MT4837 |
sma-3(e491) lin-39(n1490) III. |
Small. Vulvaless (temperature sensitive-91% Vul at 15C and 100% Vul at 20C and 25C). n1490 is also temperature sensitive Unc. |
MT5101 |
lin-39(n2110) III. |
Vulvaless. |
MT5265 |
lin-39(n2010) III. |
Vul. Strong loss of function allele. |
MT7236 |
lin-39(n1760) egl-5(n945) III. |
n1760: strong allele of lin-39, vulvaless (n300-like). n945: HSN-. Egl. Coiler. |
MT7238 |
sma-3(e491) mab-5(e1239) egl-5(n945)/lin-39(n1760) mab-5(e1239) egl-5(n945) III. |
|
MT7255 |
lin-39(n1760) mab-5(e1239) egl-5(n945) III. |
Small brood size. Semi-sterile. n945: HSN-. Egl. Coiler. |
MT7419 |
lin-39(n1760) mab-5(e1239)/sma-3(e491) mab-5(e1239) III. |
|
MT7594 |
lin-39(n1760) ncl-1(e1865) unc-36(e251) III; sDp3 (III;f). |
Pick WT to maintain strain. Dp lost at high frequency, usable for mosaic analysis. Animals without the Dp are Unc, Vul and Ncl. |
VC1858 |
lin-39(gk893) III. |
C07H6.7. External left primer: GGACCCGAAATGTTTCAAGA. External right primer: CCGTTATTCTGCCGATCATT. Internal left primer: TCAGCGCTTTGCAGAAACTA. Internal right primer: CGAAATTGCTGAGTTCGTCA. Internal WT amplicon: 1900 bp. Deletion size: 1206 bp. Deletion left flank: GGAGCTTCCTAACTATAACGCTCCAACTCT. Deletion right flank: GCATTCATCAAAAGGAATTAGATCAACCTA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807 |