Gene Information: lin-39

Namelin-39 View on WormBase
Species C. elegans
SequenceC07H6.7
Genetic positionIII:-0.67 +/- 0.004 cM
Genomic positionIII: 7528641..7536632

Strains carrying this gene

Strain Genotype Description
CF439 lin-39(n1760) III; dpy-20(e1282) IV; him-5(e1490) V; muIs23. muIs23 [hsp::lin-39 + (pMH86) dpy-20(+)]. Heat-shock inducible lin-39. muIs23 is a spontaneous integrant whose chromosomal location is unknown.
KRA102 lin-39(kas1) III; ynIs40 V; unc-3(n3435) X. ynIs40 [flp-11p::GFP] V. kas1 is a point mutation in the second intron splice acceptor site, disrupting splicing. Worms are vulvaless and have severe locomotion defects. Reference: Feng W, et al. Elife. 2020 Jan 3;9. pii: e50065. doi: 10.7554/eLife.50065.
KRA467 lin-39(kas9[lin-39::mNG::AID]) III. mNeonGreen::3xFLAG::AID was inserted at the C-terminus of the endogenous lin-39 locus by CRISPR. Endogenous lin-39 expression marked by mNG. LIN-39 protein can be degraded by Auxin application with expression of TIR-1 protein. Reference: Feng W, et al. Elife. 2020 Jan 3;9. pii: e50065. doi: 10.7554/eLife.50065.
MT1514 lin-39(n709) III. Temperature sensitive Vul. 12% Vul at 15C and 45% Vul at 25C. VC's die. Variably Egl. Variably abnormal vulval divisions. See also WBPaper00001768
MT4007 lin-39(n1760) III. Vulvaless. Strong loss of function allele.
MT4009 lin-39(n1760)/dpy-17(e164) unc-32(e189) III. Heterozygotes are WT and segregate WT, DpyUnc and Vul. Pick WT to maintain.
MT4306 lin-39(n1490) unc-36(e251) III. Unc. Vulvaless (temperature sensitive-91% Vul at 15C and 100% Vul at 20C and 25C). n1490 is also temperature sensitive Unc.
MT4491 lin-39(n1872) III. Vulvaless. Temperature sensitive. Suppressor of n765.
MT4498 lin-39(n1880) III. Vulvaless. Strong loss of function allele.
MT4500 lin-39(n1880)/dpy-17(e164) unc-32(e189) III. Heterozygotes are WT and segregate WT, DpyUnc and Vul. Pick WT to maintain.
MT4837 sma-3(e491) lin-39(n1490) III. Small. Vulvaless (temperature sensitive-91% Vul at 15C and 100% Vul at 20C and 25C). n1490 is also temperature sensitive Unc.
MT5101 lin-39(n2110) III. Vulvaless.
MT5265 lin-39(n2010) III. Vul. Strong loss of function allele.
MT7236 lin-39(n1760) egl-5(n945) III. n1760: strong allele of lin-39, vulvaless (n300-like). n945: HSN-. Egl. Coiler.
MT7238 sma-3(e491) mab-5(e1239) egl-5(n945)/lin-39(n1760) mab-5(e1239) egl-5(n945) III.
MT7255 lin-39(n1760) mab-5(e1239) egl-5(n945) III. Small brood size. Semi-sterile. n945: HSN-. Egl. Coiler.
MT7419 lin-39(n1760) mab-5(e1239)/sma-3(e491) mab-5(e1239) III.
MT7594 lin-39(n1760) ncl-1(e1865) unc-36(e251) III; sDp3 (III;f). Pick WT to maintain strain. Dp lost at high frequency, usable for mosaic analysis. Animals without the Dp are Unc, Vul and Ncl.
VC1858 lin-39(gk893) III. C07H6.7. External left primer: GGACCCGAAATGTTTCAAGA. External right primer: CCGTTATTCTGCCGATCATT. Internal left primer: TCAGCGCTTTGCAGAAACTA. Internal right primer: CGAAATTGCTGAGTTCGTCA. Internal WT amplicon: 1900 bp. Deletion size: 1206 bp. Deletion left flank: GGAGCTTCCTAACTATAACGCTCCAACTCT. Deletion right flank: GCATTCATCAAAAGGAATTAGATCAACCTA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807