Gene Information: egl-20

Nameegl-20 View on WormBase
Species C. elegans
SequenceW08D2.1
Genetic positionIV:4.42 +/- 0.001 cM
Genomic positionIV: 9812357..9815532

Strains carrying this gene

Strain Genotype Description
CF1135 egl-20(n585) IV; muEx68. muEx68 [(pJW33) myo-2p::egl-20::GFP + (7PD10.46) unc-22 (antisense)]. Use nicotine or levamisole to pick twitchers. Reference: Whangbo J & Kenyon C, (1999) Mol Cell 4(5):851-8.
CF1170 egl-20(n585) IV; muEx79. muEx79 [(pJW33) myo-2p::egl-20::GFP + (7PD10.46) unc-22 (antisense)]. Use nicotine or levamisole to pick twitchers. Reference: Whangbo J & Kenyon C, (1999) Mol Cell 4(5):851-8.
CF263 egl-20(mu39) IV. QL migration defect. HSN migratin defect.
CF980 egl-20(n585) IV; bar-1(mu349) X. Egl. QL descendants in a mutant position (anterior of ALM). Okay to grow at 15 or 20C.
EW77 lin-44(n1792) I; egl-20(n585) IV/nT1 [qIs51] (IV;V); dpy-11(e1180) mom-2(or42) V/nT1 [qIs51] (IV;V).
EW79 cwn-1(ok546) II; egl-20(n585) IV/nT1 [qIs51] (IV;V); dpy-11(e1180) mom-2(or42) V/nT1 [qIs51] (IV;V). Heterozygotes are WT and GFP+. Segregate more WT and GFP+, Dpys, and dead eggs. Dpys give only embryonic lethals.
HS1675 egl-20(n585) cwn-2(ok895) IV. Egl. Unc. Reference: Yamamoto Y, et al. PLoS Genet. 2011 Oct;7(10):e1002308.
HS1680 lin-44(n1792) zdIs5 I; cwn-1(ok546) II; egl-20(n585) cwn-2(ok895) IV/nT1 [qIs51] (IV;V); mom-2(ne874) V/nT1. zdIs5 [mec-4p::GFP + lin-15(+)] I. Homozygous nT1[qIs51] are inviable. mec-4::GFP is expressed in touch neurons. Heterozygotes are strong Egl Psa GFP+ and segregate dead eggs and non-GFP Unc Egl Psa that give only dead eggs at 25C.
HS2326 cwn-1(ok546) II; egl-20(n585) cwn-2(ok895) IV/nT1 [qIs51] (IV;V); vpIs1 X. vpIs1 [elt-3::GFP + lin-15(+)] X. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP ok546 homozygotes (Unc Egl). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Yamamoto Y, et al. PLoS Genet. 2011 Oct;7(10):e1002308.
KN369 muIs32 II; egl-20(hu105) IV. muIs32 [mec-7p::GFP + lin-15(+)]. Egl. Anterior migration of the QL.d, defects in QR.d and HSN migration.
KN387 muIs32 II; egl-20(hu120) IV. muIs32 [mec-7p::GFP + lin-15(+)]. Egl. Anterior migration of the QL.d, defects in QR.d and HSN migration.
KN594 cwn-1(ok546) II; egl-20(n585) IV.
KS46 lin-44(n1792) I; egl-20(n585) IV.
LE3845 rdvIs1 III; egl-20(gk453010) IV; lqIs58 V. rdvIs1 [egl-17p::Myri-mCherry::pie-1 3'UTR + egl-17p::mig-10::YFP::unc-54 3'UTR + egl-17p::mCherry-TEV-S::his-24 + rol-6(su1006)] III. Rollers, red fluorescence in vulvae. YFP cannot be detected. lqIs58 [gcy-32::CFP] V. Reference: Josephson MP, et al. PLoS One. 2016 Feb 10;11(2):e0148658.
MT1215 egl-20(n585) IV. Egg laying defective. Retains late stage eggs. partially ts