| CE1047 |
egl-30(ep271) I. |
Dominant, gain-of-function mutation in egl-30; missense M244I. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. |
| CG21 |
egl-30(tg26) I; him-5(e1490) V. |
Hyperactive. Small. Spicule protruder. Coiler. Throws males. |
| DA1077 |
egl-30(ad810) dpy-5(e61)/szT1 [lon-2(e678)] I; +/szT1 X. |
ad810 is homozygous lethal. ad810/+ is Egl and it suppresses gpb-2 (a.k.a. eat-11). In DA1077, heterozygotes are Egl. Strain throws Lon Males. |
| DA1084 |
egl-30(ad806) I. |
Egl. Semi-dominant suppressor of eat-11. |
| DA1096 |
egl-30(ad810)/szT1 [lon-2(e678)] I; +/szT1 X. |
ad810 is homozygous lethal. ad810/+ is Egl and it suppresses gpb-2 (a.k.a. eat-11). In DA1096, heterozygotes are Egl. Throws Lon males. |
| DA810 |
egl-30(ad810) gpb-2(ad541)/gpb-2(ad541) I. |
ad810 is homozygous lethal. ad810/+ is Egl and it suppresses gpb-2. gpb-2 phenotype is rather subtle: they are slightly starved, slightly longer than normal, and tend to be loopy in their movements (they make abnormally deep bends). Hets should be Egl and non-Eat. On most E. coli strains gpb-2 grows rather poorly, especially if the plates are older so that there is a thick and tough lawn. On such plates there will be a lot of gpb-2 larval arrest, and those that don't arrest will grow slowly. The hets should easily outgrow the gpb-2 homozygotes. [gpb-2 is also hypersensitive to the drug arecoline: they won't grow on 5 mM. The hets will grow even better than WT on 5 mM arecoline.] gpb-2(ad541) previously called eat-11(ad541). |
| DA823 |
egl-30(ad805) I. |
Suppressor of gpb-2 (a.k.a. eat-11) arecoline hypersensitivity. Unc. Egl. |
| DG2913 |
egl-30(ad810) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). |
Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ad810 homozygotes. Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. |
| JJ478 |
mex-3(zu155) egl-30(n686)/hT1 I; +/hT1 V. |
Heterozygotes are WT and segregate WT, Egls which give only dead eggs, dead eggs, and mid-larval lethals (hT1 homozygotes) |
| MT1434 |
egl-30(n686) I. |
Egg-laying defective. Retains late stage eggs. Semi-dominant. Unc-slow moving. |
| MT1520 |
egl-30(n715) I. |
Egl-forms bags of worms. Semidominant. Paralysed. Grows best at 15C. |
| MT2609 |
egl-30(n715n1190) I. |
Non-Egl. |
| NM1380 |
egl-30(js126) I. |
Suppresses unc-64(e246). |
| PS4263 |
egl-30(md186) I; dpy-20(e1282) IV; syIs105. |
syIs105 [egl-30::GFP + dpy-20(+)]. Translational fusion contains all of the presumptive 5'-transcriptional regulatory sequences, introns, and presumptive 3 regulatory sequences for egl-30, in addition to the coding sequences for GFP just 5' of the egl-30 initiating methionine. syIs105 was found to partially rescue egl-30(md186) with respect to egg laying, movement, pharyngeal pumping, and response to neurotransmitters in egg-laying assays. |
| PS4264 |
egl-30(sy676md186) I; him-5(e1490) V. |
Males mate better as heterozygotes. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. |
| VC1160 |
egl-30&emr-1(ok252) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). |
M01D7.7, M01D7.6. Homozygous viable deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ok252 homozygotes (phenotype uncharacterized). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807 |