| CB5009 |
let-552(e2542)/dpy-25(e817) II. |
At 20C or 25C heterozygotes are semi-Dpy and segregate semi-Dpys (heterozygotes), Dpys (dpy-25/dpy-25) and animals which arrest as Dpyish young larvae (let-552/let-552). Maintain by picking semi-Dpy. At 15C it is difficult to distinguish between let-552 homozygotes and dpy-25 homozygotes (e817 is inviable at 15C). |
| DR2054 |
mIn1 [unc-4(e120) dpy-10(e128)]/let-552(e2542) rol-1(e91) II. |
Heterozygotes are WT and segregate WT, DpyUnc [mIn1(mc6) homozygotes], and two-fold arrest Let Rol progeny. Well balanced. Male stock. Cross WT males to WT hermaphrodites and check for correct segregation of progeny. |
| DR2075 |
mIn1 [unc-4(e120)]/let-552(e2542) rol-1(e91) II. |
Heterozygotes are WT and segregate WT, Lethal Rollers (arrested 2-fold hatchlings), and Unc-4 mIn1(mC6) homozygotes. Pick WT and check for correct segregation of progeny. |
| DR2102 |
mIn1 [rol-1(e91)]/let-552(e2542) unc-4(e120) II. |
Heterozygotes are WT and segregate WT, Lethal Uncs and Roller mIn1 homozygotes. let-552 unc-4 chromosome is well balanced. Rol does not express until late L4/early adult. Pick WT and check for correct segregation of progeny. |
| DR2150 |
mIn1 [rol-1(e91) dpy-10(e128)]/let-552(e2542) unc-4(e120) II. |
Heterozygotes are WT and segregate WT, DpyRol mIn1 homozygotes and 2-fold arrest Unc larvae. Male stock; mate WT males and WT hermaphrodites to maintain. |