Gene Information: lin-5

Namelin-5 View on WormBase
Species C. elegans
SequenceT09A5.10
Genetic positionII:0.59 +/- 0.001 cM
Genomic positionII: 7860574..7863563

Strains carrying this gene

Strain Genotype Description
CB2744 lin-5(e1348)/dpy-10(e128) II. Heterozygotes are WT. Segregates Dpy. Segregates sterile Unc--Unc cannot back. Balanced well.
LP585 lin-5(cp288[lin-5::mNG-C1^3xFlag]) II. FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press.
SV122 lin-5(n3070)/mnC1 [dpy-10(e128) unc-52(e444)] II. Heterozygotes are WT and segregate WT, Stu and DpyUncs. n3070 is a strong loss-of-function or null allele. Molecular lesion: P to S at position 24 as well as an amber mutation terminating translation after amino acid 52. DNA replication continues in the absence of mitosis. Mutants enter mitotis at the normal time and form bipolar spindles, but fail chromosome alignment at the metaphase plate, sister chromatid separation and cytokinesis.
SV123 lin-5(n3066)/mnC1 [dpy-10(e128) unc-52(e444)] II. Heterozygotes are WT and segregate WT, Stu and DpyUncs. n3066 is a strong loss-of-function or null allele. Molecular lesion: ochre mutation terminating translation at amino acid 538. DNA replication continues in the absence of mitosis. Mutants enter mitotis at the normal time and form bipolar spindles, but fail chromosome alignment at the metaphase plate, sister chromatid separation and cytokinesis.
SV124 lin-5(ev571) II. Temperature sensitive - maintain at 15C. Recessive loss-of-function stronger with increases in temperature, nearly WT at 15C. At the non-permissive temperature DNA replication continues in the absence of mitosis. Mutants enter mitosis at the normal time and form bipolar spindles, but fail chromosome alignment at the metaphase plate, sister chromatid separation and cytokinesis. Molecular lesion is a 9 bp duplication followed by a T to C transversion. Mutagen was EMS but mutation likely caused by polymerase slippage.
SV13 lin-5(e1348)/mnC1 [dpy-10(e128) unc-52(e444)] II. Heterozygotes are WT and segregate WT, Stu and DpyUncs. Molecular lesion: amber mutation terminating translation at amino acid 159. DNA replication continues in the absence of mitosis. Mutants enter mitotis at the normal time and form bipolar spindles, but fail chromosome alignment at the metaphase plate, sister chromatid separation and cytokinesis.
SV46 lin-5(e1457)/mnC1 [dpy-10(e128) unc-52(e444)] II. Heterozygotes are WT and segregate WT, Stu and DpyUncs. e1457 is a strong loss-of-function or null allele. Molecular lesion: G to E at position 40. DNA replication continues in the absence of mitosis. Mutants enter mitotis at the normal time and form bipolar spindles, but fail chromosome alignment at the metaphase plate, sister chromatid separation and cytokinesis.