| DR608 |
daf-2(m212) III. |
Temperature sensitive Daf-c. Maintain at 15C. Adults Age and Itt, but not Unc (severe class 1 allele). m212 results in an amino acid substitution(C883Y) in the extracellular portion of the DAF-2 receptor (specificaly, the FnIII2ID domain). |
| DR629 |
unc-15(e73) I; sus-1(m156) daf-2(e1370) III; eDp23 V. |
Temperature sensitive dauers. Paralyzed Unc. sus-1 suppresses the ability of eDp23 to suppress unc-15. eDp23 = sup-3(e1407). |
| DV2275 |
daf-2(e1370) dpy-17(e164) III. |
|
| ENL21 |
daf-2(e1370) mir-80(nDf53) III; mir-58.1(n4640) IV; mir-81&mir-82(nDf54) X. |
Maintain at 15C. Temperature-sensitive dauer constitutive (daf-c). |
| ENL54 |
daf-2(e1370) III; mir-58.1(n4640) IV; mir-81&mir-82(nDf54) X. |
Maintain at 15C. Temperature-sensitive dauer constitutive. |
| ENL55 |
daf-2(e1370) mir-80(nDf53) III; mir-81&mir-82(nDf54) X. |
Maintain at 15C. Temperature-sensitive dauer constitutive. |
| ENL70 |
daf-2(e1370) III; sma-10(ok2224) IV. |
Derived from RB1739 and CB1370. |
| GA132 |
daf-16(mgDf50) I; daf-2(m646) III. |
Maintain at 15C. |
| GA158 |
daf-16(mgDf50) I; daf-2(m65) III. |
Maintain at 15C. |
| GA2002 |
daf-2(e1370) III; wuIs305. |
wuIs305 [myo-3p::Queen-2m]. Temperature sensitive dauer constitutive. Maintain at 15C. ATP sensor Queen-2m is expressed under the control of myo-3 promoter. Reference: Galimov et al. Cell Rep. 2018 Mar 6;22(10):2730-2741. |
| GA2003 |
daf-16(mgDf50) I; daf-2(e1370) III; wuIs305. |
wuIs305 [myo-3p::Queen-2m]. ATP sensor Queen-2m is expressed under the control of myo-3 promoter. Reference: Galimov et al. Cell Rep. 2018 Mar 6;22(10):2730-2741. |
| GR1308 |
daf-16(mg54) I; daf-2(e1370) III. |
mg54 almost completely suppresses the daf-c phenotype of daf-2. 0.4% dauers. |
| GR1309 |
daf-16(mgDf47) I; daf-2(e1370) III. |
mgDf47 completely suppresses daf-c phenotype of daf-2. mgDf47 deletes approximately 8kb of the daf-16 gene beginning after exon 4. |
| GR1336 |
daf-2(e1370) III; njEx32. |
njEx32 [ges-1p::daf-2(+) + ges-1p::GFP + rol-6(su1006)]. Pick Rollers to maintain. Reference: Wolkow CA, et al. Science. 2000 Oct 6;290(5489):147-50. |
| GR1337 |
daf-2(e1370) III; njEx38. |
njEx38 [unc-54p::daf-2(cDNA)::unc-54 3'UTR + unc-54p::GFP + rol-6(su1006)]. Pick Rollers to maintain. Reference: Wolkow CA, et al. Science. 2000 Oct 6;290(5489):147-50. |
| GR1339 |
daf-2(e1370) III; mgEx376. |
mgEx376 [unc-14p::daf-2 + rol-6(su1006)]. Pick Rollers to maintain. Reference: Wolkow CA, et al. Science. 2000 Oct 6;290(5489):147-50. |
| GR1340 |
daf-2(e1370) III; mgEx373. |
mgEx373 [unc-119p::daf-2(cDNA)::unc-54 3'UTR + rol-6(su1006)]. Pick Rollers to maintain. Reference: Wolkow CA, et al. Science. 2000 Oct 6;290(5489):147-50. |
| GR1895 |
daf-2(e1370) III; mgIs67. |
mgIs67 [daf-16p::daf-16::GFP + rol-6(su1006)]. Temperature-sensitive. Daf-c. Maintain at 15C. Dauer formation at 25C. Slow growing. Dauer-like at 20C. DAF-16::GFP is fully nuclear at 20C. Reference: Riedel CG, et al. Nat Cell Biol. 2013 May;15(5):491-501. |
| GR2107 |
daf-2(e1370) III; mgEx371. |
mgEx371 [dpy-30p::daf-2(cDNA)::unc-54 3'UTR + rol-6(su1006)]. Pick Rollers to maintain. Reference: Wolkow CA, et al. Science. 2000 Oct 6;290(5489):147-50. |
| GR2117 |
daf-2(e1365) III; mgIs35. |
mgIs35 [ins-1(genomic) + mec-7p::GFP]. Temperature-sensitive. Maintain at 15C. Daf-c. High penetrance of dauer formation at 20C. Integration of mgEx557. Reference: Pierce SB, et al. Genes Dev. 2001 Mar 15;15(6):672-86. |
| HT1881 |
daf-16(mgDf50) I; daf-2(e1370) unc-119(ed3) III; lpIs12. |
lpIs12 [daf-16a::RFP + unc-119(+)]. Maintain at 15C. Extended lifespan. Reference: Kwon ES, et al., Nature. 2010 Jul 22;466(7305):498-502. |
| HT1882 |
daf-16(mgDf50) I; daf-2(e1370) unc-119(ed3) III; lpIs13. |
lpIs13 [daf-16b::CFP + unc-119(+)]. Maintain at 15C. Extended lifespan. Reference: Kwon ES, et al., Nature. 2010 Jul 22;466(7305):498-502. |
| HT1883 |
daf-16(mgDf50) I; daf-2(e1370) unc-119(ed3) III; lpIs14. |
lpIs14 [daf-16f::GFP + unc-119(+)]. Maintain at 15C. Extended lifespan. Reference: Kwon ES, et al., Nature. 2010 Jul 22;466(7305):498-502. |
| HT1890 |
daf-16(mgDf50) I; daf-2(e1370) III. |
Short-lived. Dauer-defective (Daf-d). Maintain under standard conditions. Reference: Kwon ES, et al. Nature. 2010 Jul 22;466(7305):498-502. |
| JK1438 |
daf-2(m65)/qC1 [dpy-19(e1259) glp-1(q339)] III. |
Heterozygotes are WT and segregate WT, non-conditional dauers, and Sterile Dpys. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. |
| JT193 |
daf-2(sa193) III. |
Adults Age and Itt, but not Unc (class 1 allele). sa193 results in an amino acid substitution(A580T) in the extracellular portion of the DAF-2 receptor (specifically, the L2 domain). |
| MAH14 |
daf-2(e1370) III; adIs2122. |
adIs2122 [lgg-1::GFP + rol-6(su1006)]. Rollers. Maintain at 15C. ~30% animals form dauers at 20C. Not all animals will roll, but all should be GFP+. Reference: Egan DF, et al. Science. 2011 Jan 28;331(6016):456-61. |
| MQ513 |
daf-2(e1370) clk-1(e2519) III. |
Daf-c at 25C. Slower growing than clk-1(e2519). |
| MQD774 |
daf-2(e1370) III; hqIs180. |
hqIs180 [sdhb-1p::mtLS::cpYFP + rol-6(su1006)]. Rollers. About two mitoflash events per anterior pharynx per 200 seconds on adult day 3 when cultured on standard NGM plates at 20 ºC. In hqIs180, mtLS is a mitochondrial localization sequence from SDHB-1 and cpYFP is circularly permuted yellow fluorescent protein, a superoxide sensor (Wang W. et al., Cell, 2008). The cpYFP signal is detected in most or all tissues, but most strongly in the pharyngeal muscles. Reference: Shen E-Z, et al. Nature. 2014 Feb 12. doi: 10.1038/nature13012. |
| MQD812 |
daf-16(mu86) I; daf-2(e1370) III; hqIs180. |
hqIs180 [sdhb-1p::mtLS::cpYFP + rol-6(su1006)]. Rollers. About three mitoflash events per anterior pharynx per 200 seconds on adult day 3 when cultured on standard NGM plates at 20 ºC. In hqIs180, mtLS is a mitochondrial localization sequence from SDHB-1 and cpYFP is circularly permuted yellow fluorescent protein, a superoxide sensor (Wang W. et al., Cell, 2008). The cpYFP signal is detected in most or all tissues, but most strongly in the pharyngeal muscles. Reference: Shen E-Z, et al. Nature. 2014 Feb 12. doi: 10.1038/nature13012. |
| MR905 |
daf-2(e1370) aakb-2(rr88) III. |
Daf-C at 25C. |
| MT3363 |
daf-2(e1370) dpy-17(e164) III. |
Dpy. Daf-C. Maintain at 15C. |
| OH14654 |
daf-16(ot853[daf-16::mNeonGreen::3xFlag::AID]) I; daf-2(e1370) III. |
Temperature sensitive dauer constitutive. Maintain at 15C. CRISPR/Cas9-engineered AID conditional daf-16 allele in daf-2(e1370) background (TIR1-less control). Reference: Aghayeva et al., submitted |
| OH14888 |
daf-16(ot853[daf-16::mNG::3xFlag::AID]) I; ieSi57 II; daf-2(e1370) III. |
ieSi57 [eft-3p::TIR1::mRuby::unc-54 3'UTR] II. Maintain at 15C. Temperature-sensitive dauer constitutive. CRISPR/Cas9-engineered AID conditional daf-16 allele in daf-2(e1370) background with ubiquitous TIR1 expression. Reference: Aghayeva et al., submitted |
| OH14897 |
daf-16(ot853[daf-16::mNeonGreen::3xFlag::AID]) I; ieSi60 II; daf-2(e1370) III. |
ieSi60 [myo-2p::TIR1::mRuby::unc-54 3'UTR] II. Temperature sensitive dauer constitutive. Maintain at 15C. Pharyngeal muscle-specific depletion of DAF-16 in the presence of auxin. Reference: Aghayeva et al., submitted |
| OH14945 |
daf-16(ot853[daf-16::mNeonGreen::3xFlag::AID]) I; ieSi61 II; daf-2(e1370) III. |
ieSi61[ges-1p::TIR1::mRuby + unc-119(+)] II. Temperature sensitive dauer constitutive. Maintain at 15C. Intestine-specific depletion of DAF-16 in the presence of auxin. Reference: Aghayeva et al., submitted |
| OH15845 |
daf-16(ot853[daf-16::mNG::AID]) I; daf-2(e1370) III; otIs730. |
otIs730 [UPN::TIR1::mTur2 + inx-6(prom18)::TagRFP]. Temperature sensitive dauer constitutive. Maintain at 15C. UPN (Ultra Pan-Neuronal) promoter contains four short pan-neuronal promoters fused together (unc-11::rgef-1::ehs-1::ric-19). Panneuronal depletion of DAF-16 in the presence of auxin. Reference: Aghayeva et al., submitted |
| OH16508 |
daf-16(ot975[daf-16::mNeptune2.5::3xFlag::AID]) I; daf-2(e1370) III; otIs730. |
otIs730 [UPN::TIR1::mTur2 + inx-6(prom18)::TagRFP]. Temperature sensitive dauer constitutive. Maintain at 15C. UPN (Ultra Pan-Neuronal) promoter contains four short pan-neuronal promoters fused together (unc-11::rgef-1::ehs-1::ric-19). Panneuronal depletion of DAF-16 in the presence of auxin. Reference: Aghayeva et al., submitted |
| PJ1146 |
daf-2(m41) III; ccIs55 V; pdk-1(mg142) X. |
ccIs55 [unc-54::lacZ + sup-7(st5)] V. Gain of function allele of pdk-1. |
| PJ1166 |
daf-2(m41) III; ccIs55 V; njEx38. |
ccIs55 [unc-54::lacZ + sup-7(st5)] V. njEx38 [unc-54p::daf-2(+) + goa-1p::GFP + rol-6(su1006)]. Maintain by picking Rollers. Arrest as dauers at 25C. Maintain at 15C. |
| PJ1700 |
daf-2(m41) III; unc-51(e369) ccIs55 V. |
ccIs55 [unc-54::lacZ + sup-7(st5)] V. Unc -- very slow. Grows very slowly. |
| PS5531 |
Cbr-daf-2(sy5445). |
This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. |
| QQ202 |
daf-2(cv20[daf-2::gfp]) III. |
Superficially wild-type. Reference: Simske J & Dong Y. (2017). International Worm Meeting. The role of DAF-2 In the transmission of maternal and paternal nutritional status during embryogenesis. WBPaper00051789. |
| QQ253 |
daf-16(mgDf50) I; daf-2(m65) III; zuIs45 V. |
zuIs45 [nmy-2p::nmy-2::GFP + unc-119(+)] V. Maintain at 15C. Derived from parental strains GA158 and JJ1473. Reference: Simske J & Dong Y. 2017). The role of DAF-2 In the transmission of maternal and paternal nutritional status during embryogenesis presented in International Worm Meeting. |
| SD1625 |
egl-27(we3) II; daf-2(e1370) III. |
Maintain at 20C. Embryonic lethal at 15C. Dauer formation at 25C. Reference: Xu X, Kim SK. PLoS Genet. 2012;8(12):e1003108. |
| SD1743 |
egl-27(we3) II; daf-2(e1370) III; muIs84. |
muIs84 [sod-3p::GFP + rol-6(su1006)]. Maintain at 20C. Embryonic lethal at 15C. Dauer formation at 25C. GFP should be visible at low magnification. Reference: Xu X, Kim SK. PLoS Genet. 2012;8(12):e1003108. |
| SD1776 |
daf-2(e1370) III; ccIs4251; stIs10161. |
ccIs4251 [(pSAK2) myo-3p::GFP::LacZ::NLS + (pSAK4) myo-3p::mitochondrial GFP + dpy-20(+)] I. stIs10161 [egl-27p::HIS-24::mCherry + unc-119(+)]. Maintain at 20C or lower. Dauer formation at 25C. mCherry visible at low magnification. Reference: Xu X, Kim SK. PLoS Genet. 2012;8(12):e1003108. |
| SD1777 |
daf-2(e1370) III; elt-3(vp1) X; stIs10161. |
stIs10161 [egl-27p::HIS-24::mCherry + unc-119(+)]. Maintain at 20C or lower. Dauer formation at 25C. mCherry visible at low magnification. Reference: Xu X, Kim SK. PLoS Genet. 2012;8(12):e1003108. |
| SD1778 |
daf-16(mu86) I; daf-2(e1370) III; stIs10161. |
stIs10161 [egl-27p::HIS-24::mCherry + unc-119(+)]. mCherry visible at low magnification. Reference: Xu X, Kim SK. PLoS Genet. 2012;8(12):e1003108. |
| TM127 |
daf-2(e1370) III; sod-3(sj134) X. |
High frequency of dauer formation under hyperoxia (90% oxygen) at 20C. Reference: Honda Y, Tanaka M, Honda S, (2008) Exp Gerontol 43:520-9. |