| AMH30 |
juIs76 II; daf-2(e1370) III. |
juIs76 [unc-25p::GFP + lin-15(+)] II. Maintain at 15C. Temperature-sensitive dauer constitutive. |
| AMH36 |
juIs76 II; daf-2(e1370) III; unc-33(mn407) IV. |
juIs76 [unc-25p::GFP + lin-15(+)] II. Unc. Maintain at 15C. Synthetic Lethality at 25C. |
| AMH38 |
juIs76 II; daf-2(e1370) III; unc-33(e204) IV. |
juIs76 [unc-25p::GFP + lin-15(+)] II. |
| AMH46 |
juIs76 II; daf-2(e1370) III; unc-33(e1193) IV. |
Maintain at 15C. Synthetic Lethality at 25C. Unc; almost paralyzed. Growth slow. |
| AMH55 |
daf-2(e1370) III; otIs117 IV. |
otIs117 [unc-33p::GFP + unc-4(+)] IV. Maintain at 15C. Temperature-sensitive dauer constitutive. Pan-neuronal GFP. |
| AMH67 |
unc-33(e204) IV; daf-2(e1370) III. |
Maintain at 15C. Synthetic Lethality at 25C. |
| AMH69 |
unc-33(mn407) IV; daf-2(e1370) III. |
Maintain at 15C. Synthetic Lethality at 25C. |
| AMH71 |
unc-33(e1193) IV; daf-2(e1370) III. |
Maintain at 15C. Synthetic Lethality at 25C. |
| AUM2023 |
daf-2(e1370) unc-119(ed3) III; vizIs23. |
vizIs23 [pie-1p::GFP::daf-2(WT)::pie-1 3'UTR + unc-119(+)]. Maintain at 15C; pick superficially wild-type animals to avoid silencing of the transgene. pie-1 driven DAF-2 coding region with GFP transgene rescues the germline defects of daf-2(e1370). Slow growing. The transgene is sometimes silenced in the germline resulting in dauerunc animals at 25C. Reference: Lopez AL 3rd, et al. Dev Cell. 2013 Oct 28;27(2):227-40. |
| CB1370 |
daf-2(e1370) III. |
Temperature sensitive dauer constitutive. Maintain at 15C. 100% dauers at 25C. 15% dauer formation at 20C. Long-lived. M-MATING+LOW TEMPERATURE ONLY. |
| CF1137 |
daf-16(mu86) I; daf-2(e1370) III; muIs61. |
muIs61 [daf-16a::GFP + rol-6(su1006)]. Temperature-sensitive. Maintain at 15 C. Rollers. Reference: Lin K, Hsin H, Libina N, Kenyon C. Nat Genet. 2001 Jun;28(2):139-45. |
| CF1295 |
daf-16(mu86) I; daf-2(e1370) III; muEx108. |
muEx108 [(pKL99-2) daf-16::GFP/daf16bKO + rol-6(su1006)]. Grows okay at 20C. Rollers should form dauers at 25C. Pick Rollers to maintain. |
| CF1380 |
daf-16(mu86) I; daf-2(e1370) III; muEx158. |
muEx158 [daf-16cAM::GFP + sur-5p::GFP] (AM = AKT-site mutant). Pick GFP+ worms to maintain. Sterile at 25C; grow at 20C or less. muEx158 contains GFP-tagged daf-16 c isoform (described as a1 isoform in Lin, et al. Nat Genet. 2001) with 4 Ser/Thr residues mutated to Ala, which completely rescues dauer formation and partially restores longevity of daf-16; daf-2 double mutants. Reference: Lin K, et al. Nat Genet. 2001 Jun;28(2):139-45. |
| CF1442 |
daf-16(mu86) I; daf-2(e1370) III; muEx169. |
muEx169 [unc-119p::GFP::daf-16 + rol-6(su1006)]. Pick Rollers to maintain. May grow better at 15C. |
| CF1449 |
daf-16(mu86) I; daf-2(e1370) III; muEx176. |
muEx176 [daf-16p::GFP::daf-16 + rol-6(su1006)]. Pick rollers to maintain -- Low transmission rate! Maintain at 15C. Forms dauers at 25C. Reference: Lin K, et al. Nat Genet. 2001 Jun;28(2):139-45. |
| CF1514 |
daf-16(mu86) I; daf-2(e1370) III; muEx211. |
muEx211[pNL213(ges-1p::GFP::daf-16) + rol-6(su1006)]. Grows at 15C (probably also at 20C). Pick Rollers to maintain. |
| CF1515 |
daf-16(mu86) I; daf-2(e1370) III; muEx212. |
muEx212[pNL212(myo-3p::GFP::daf-16) + rol-6(su1006)]. Grows at 15C (probably also at 20C). Pick Rollers to maintain. |
| CF1580 |
daf-2(e1370) III; muIs84. |
muIs84 [(pAD76) sod-3p::GFP + rol-6(su1006)]. Green expression in head, tail and around vulva. Daf-C at 25C. Grows well at 20C. |
| CF1588 |
daf-16(mu86) I; daf-2(e1370) III; muIs84. |
muIs84 [(pAD76) sod-3p::GFP + rol-6(su1006)]. Dim green expression in head, tail and around vulva. Daf-d. Can grow at 20C. |
| CF1595 |
daf-16(mu86) I; daf-2(e1370) III; muEx227. |
muEx227 [(pNL213) ges-1p::GFP::daf-16) + rol-6(su1006)]. Pick Rollers to maintain. |
| CF1660 |
daf-16(mu86) I; daf-2(e1370) III; muIs84; muEx211. |
muIs84 [(pAD76) sod-3p::GFP + rol-6(su1006)]. muEx211 [ges-1p::daf-16::GFP + rol-6(su1006)]. Pick Rollers to maintain. Partial rescue of lifespan phenotype. Some animals show variable daf-16 expression in the intestine. Grows okay at 15C. [NOTE: muEx211 is quite unstable. Be sure to pick Rollers to avoid losing the array.] |
| CF1724 |
daf-16(mu86) I; daf-2(e1370) III; muIs105. |
muIs105 [daf-16p::GFP::daf-16 + rol-6(su1006)]. Rollers; Rol phenotype is not always evident). Integrated line derived from CF1449. Maintain at 15C. Forms dauers at 25C. Reference: Lin K, et al. Nat Genet. 2001 Jun;28(2):139-45. |
| CF1814 |
rrf-3(pk1426) II; daf-2(e1370) III. |
Daf-c at 25.5C; grow at 20C or less. Long lived. |
| CF1827 |
daf-16(mu86) I; daf-2(e1370) III; muEx268. |
muEx268 [ges-1p::GFP::daf-16(cDNA) + odr-1::RFP]. daf-16 GFP expressed in intestine. Partial rescue of lifespan phenotype. Grows okay at 15C. Pick RFP to maintain. |
| CF1844 |
rrf-3(b26) II; daf-2(mu150) III; fem-1(hc17) IV. |
Long lifespan. Maintain at 15C. Worms develop slowly at 20C, and are sterile at 25C. Reference: Garigan D, et al. Genetics. 2002 Jul;161(3):1101-12. |
| CF1980 |
rrf-3(pk1426) II; daf-2(e1368) III. |
Daf-c at 25.5C; grow at 20C or less. Long lived. |
| CF2005 |
daf-16(mu86) I; daf-2(e1370) III; muIs120. |
muIs120 [ges-1p::GFP::daf-16 + rol-6(su1006)]. Maintain at 15-20C. Rollers. Long-lived. Gamma irradiation-induced integration of muEx211. Rescues daf-16a1/c in the intestine (described in Libina et al, 2003). Reference: Zhang P, et al. Cell Metab, 2013. 17(1): p. 85-100. |
| CF2093 |
daf-16(mu86) I; daf-2(e1370) III; muIs131. |
muIs131 [unc-119p::GFP::daf-16 + rol-6(su1006)]. Maintain at 15-20C. Rollers. Modestly long-lived. Gamma irradiation-induced integration of muEx184. Rescues daf-16a1/c in neurons (described in Libina et al, 2003). Reference: Zhang P, et al. Cell Metab, 2013. 17(1): p. 85-100. |
| CF2102 |
daf-16(mu86) I; daf-2(e1370) III; muIs126. |
muIs126 [myo-3p::GFP::daf-16 + rol-6(su1006)]. Maintain at 15-20C. Rollers. Gamma irradiation-induced integration of muEx215. Rescues daf-16a1/c in muscles (described in Libina et al, 2003). Reference: Zhang P, et al. Cell Metab, 2013. 17(1): p. 85-100. |
| CF2570 |
daf-16(mu86) I; daf-2(e1370) III; muIs142. |
muIs142 [ges-1p::GFP::daf-16(cDNA) + odr-1p::RFP]. Maintain at 15-20C. Gamma irradiation-induced integration of muEx268. Rescues daf-16a1/c in the intestine (described in Libina et al, 2003). Reference: Zhang P, et al. Cell Metab, 2013. 17(1): p. 85-100. |
| CG499 |
daf-2(e1368) unc-103(n1213) III; him-5(e1490) V; rgEx178. |
rgEx178 [lev-11p::daf-2(+) + lev-11p::GFP]. rgEx178 rescues food deprivation suppression of unc-103(n1213)-induced spicule protraction. |
| CG500 |
daf-2(e1368) unc-103(n1213) III; him-5(e1490) V; rgEx180. |
rgEx180 [aex-3p::daf-2(+) + tnt-4p::GFP]. rgEx180 reduces unc-103(n1213)-induced spicule protraction, and rescues constitutive dauer formation. |
| CG640 |
daf-2(e1368) unc-103(n1213) III; him-5(e1490) V; rgEx247. |
rgEx247 [hsp-16p::daf-2(+) + lev-11p::GFP]. rgEx247 restores food-deprivation suppression of unc-103(n1213)-induced spicule protraction. |
| CS624 |
daf-2(e1370) III; lon-2(e678) X. |
Temperature-sensitive. Maintain at 15C. Lon. Daf-c. |
| DA438 |
bli-4(e937) I; rol-6(e187) II; daf-2(e1368) vab-7(e1562) III; unc-31(e928) IV; dpy-11(e224) V; lon-2(e678) X. |
Linkage mapping strain. Maintain at 15C. |
| DG2884 |
daf-2(e1370) III; fog-2(oz40) V. |
Male-female strain. Maintain by mating at 15 C. |
| DR128 |
dpy-1(e1) daf-2(e1370) III. |
Temperature sensitive dauer constitutive. Dpy. 100% dauers at 25C. |
| DR129 |
daf-2(e1370) unc-32(e189) III. |
Temperature senstive dauer constitutive. Unc-coiler. 100% dauers at 25C. |
| DR130 |
dpy-1(e1) daf-2(e1370) unc-32(e189) III. |
Temperature sensitive dauer constitutive. 100% dauers at 25C. Dpy. Unc-coiler. Reference strain for daf-2 complementation. |
| DR1309 |
daf-16(m26) I; daf-2(e1370) III. |
|
| DR1564 |
daf-2(m41) III. |
Temperature-sensitive dauer-consitutive, long-lived, intrinsically thermotolerant. Adults slightly shorter than adults of other daf-2 alleles. Up to 6% of L1s arrest at 25.5C. Makes nearly 100% dauers at 25C, 0% dauers at 15C. Good recovery of dauers at 15C. |
| DR1565 |
daf-2(m596) III. |
Class 2 allele of daf-2. Temperature sensitive Daf-c. Adults are long-lived (Age) and exhibit extrinsic thermotolerance (Itt). |
| DR1566 |
daf-2(m579) III. |
Adults Age and Itt and temperature sensitive Unc (class 2 allele). m579 results in an amino acid substitution(R437C) in the extracellular portion of the DAF-2 receptor (specifically, the Cys-rich domain). |
| DR1567 |
daf-2(m577) III. |
Class 1 allele of daf-2. Temperature sensitive Daf-c. Adults are long-lived (Age) and exhibit extrinsic thermotolerance (Itt). |
| DR1568 |
daf-2(e1371) III. |
Class 1 allele of daf-2. Temperature sensitive Daf-c. Adults are long-lived (Age) and exhibit extrinsic thermotolerance (Itt). |
| DR1572 |
daf-2(e1368) III. |
Maintain at 15C. Class 1 allele of daf-2. Temperature sensitive Daf-c. Adults are long-lived (Age) and exhibit extrinsic thermotolerance (Itt). |
| DR1574 |
daf-2(e1391) III. |
Class 2 allele of daf-2. Temperature sensitive Daf-c. Adults are long-lived (Age) and exhibit extrinsic thermotolerance (Itt). Produces some dauers at 15C. CGC received new stock 10/25/00 from David Gems. Received new stock from Patrice Albert in the Riddle lab 6/04. |
| DR1942 |
daf-2(e979) III. |
This strain forms 20% dauers at 15C. At 25C there occurs about 25% embryonic arrest and about 75% L1 arrest. The e979 mutation results in an amino acid substitution, C146Y, in the ligand-binding domain of the DAF-2 receptor. [CGC received new stock of DR1942 September 2002. Previous stock was probably m41 and not e979.] [June 2004: Patrice Albert has confirmed the mutation in this stock: Repeat of sequencing for CGC collection strain DR1942 [daf-2(e979)] is complete. The strain does carry a C146Y mutation (coding strand TGC to TAC) [Mutation position is at 143, not 146, based on the amino acid sequence shown in Wormbase for daf-2. It's the C in partial sequence EKRCGPI of Exon 5.].] |
| DR200 |
daf-2(e1370) III; daf-13(m66) X. |
Temperature sensitive dauer constitutive. Dauers SDS-sensitive. |
| DR410 |
daf-2(m65)/unc-32(e189) III. |
Heterozygotes are WT and segregate WT, dauer constitutive, and Uncs. Lethal dauers. |