Gene Information: daf-2

Namedaf-2 View on WormBase
Species C. elegans
SequenceY55D5A.5
Genetic positionIII:-8.03 +/- 0.111 cM
Genomic positionIII: 2994514..3040846

Strains carrying this gene

Strain Genotype Description
AMH30 juIs76 II; daf-2(e1370) III. juIs76 [unc-25p::GFP + lin-15(+)] II. Maintain at 15C. Temperature-sensitive dauer constitutive.
AMH36 juIs76 II; daf-2(e1370) III; unc-33(mn407) IV. juIs76 [unc-25p::GFP + lin-15(+)] II. Unc. Maintain at 15C. Synthetic Lethality at 25C.
AMH38 juIs76 II; daf-2(e1370) III; unc-33(e204) IV. juIs76 [unc-25p::GFP + lin-15(+)] II.
AMH46 juIs76 II; daf-2(e1370) III; unc-33(e1193) IV. Maintain at 15C. Synthetic Lethality at 25C. Unc; almost paralyzed. Growth slow.
AMH55 daf-2(e1370) III; otIs117 IV. otIs117 [unc-33p::GFP + unc-4(+)] IV. Maintain at 15C. Temperature-sensitive dauer constitutive. Pan-neuronal GFP.
AMH67 unc-33(e204) IV; daf-2(e1370) III. Maintain at 15C. Synthetic Lethality at 25C.
AMH69 unc-33(mn407) IV; daf-2(e1370) III. Maintain at 15C. Synthetic Lethality at 25C.
AMH71 unc-33(e1193) IV; daf-2(e1370) III. Maintain at 15C. Synthetic Lethality at 25C.
AUM2023 daf-2(e1370) unc-119(ed3) III; vizIs23. vizIs23 [pie-1p::GFP::daf-2(WT)::pie-1 3'UTR + unc-119(+)]. Maintain at 15C; pick superficially wild-type animals to avoid silencing of the transgene. pie-1 driven DAF-2 coding region with GFP transgene rescues the germline defects of daf-2(e1370). Slow growing. The transgene is sometimes silenced in the germline resulting in dauerunc animals at 25C. Reference: Lopez AL 3rd, et al. Dev Cell. 2013 Oct 28;27(2):227-40.
CB1370 daf-2(e1370) III. Temperature sensitive dauer constitutive. Maintain at 15C. 100% dauers at 25C. 15% dauer formation at 20C. Long-lived. M-MATING+LOW TEMPERATURE ONLY.
CF1137 daf-16(mu86) I; daf-2(e1370) III; muIs61. muIs61 [daf-16a::GFP + rol-6(su1006)]. Temperature-sensitive. Maintain at 15 C. Rollers. Reference: Lin K, Hsin H, Libina N, Kenyon C. Nat Genet. 2001 Jun;28(2):139-45.
CF1295 daf-16(mu86) I; daf-2(e1370) III; muEx108. muEx108 [(pKL99-2) daf-16::GFP/daf16bKO + rol-6(su1006)]. Grows okay at 20C. Rollers should form dauers at 25C. Pick Rollers to maintain.
CF1380 daf-16(mu86) I; daf-2(e1370) III; muEx158. muEx158 [daf-16cAM::GFP + sur-5p::GFP] (AM = AKT-site mutant). Pick GFP+ worms to maintain. Sterile at 25C; grow at 20C or less. muEx158 contains GFP-tagged daf-16 c isoform (described as a1 isoform in Lin, et al. Nat Genet. 2001) with 4 Ser/Thr residues mutated to Ala, which completely rescues dauer formation and partially restores longevity of daf-16; daf-2 double mutants. Reference: Lin K, et al. Nat Genet. 2001 Jun;28(2):139-45.
CF1442 daf-16(mu86) I; daf-2(e1370) III; muEx169. muEx169 [unc-119p::GFP::daf-16 + rol-6(su1006)]. Pick Rollers to maintain. May grow better at 15C.
CF1449 daf-16(mu86) I; daf-2(e1370) III; muEx176. muEx176 [daf-16p::GFP::daf-16 + rol-6(su1006)]. Pick rollers to maintain -- Low transmission rate! Maintain at 15C. Forms dauers at 25C. Reference: Lin K, et al. Nat Genet. 2001 Jun;28(2):139-45.
CF1514 daf-16(mu86) I; daf-2(e1370) III; muEx211. muEx211[pNL213(ges-1p::GFP::daf-16) + rol-6(su1006)]. Grows at 15C (probably also at 20C). Pick Rollers to maintain.
CF1515 daf-16(mu86) I; daf-2(e1370) III; muEx212. muEx212[pNL212(myo-3p::GFP::daf-16) + rol-6(su1006)]. Grows at 15C (probably also at 20C). Pick Rollers to maintain.
CF1580 daf-2(e1370) III; muIs84. muIs84 [(pAD76) sod-3p::GFP + rol-6(su1006)]. Green expression in head, tail and around vulva. Daf-C at 25C. Grows well at 20C.
CF1588 daf-16(mu86) I; daf-2(e1370) III; muIs84. muIs84 [(pAD76) sod-3p::GFP + rol-6(su1006)]. Dim green expression in head, tail and around vulva. Daf-d. Can grow at 20C.
CF1595 daf-16(mu86) I; daf-2(e1370) III; muEx227. muEx227 [(pNL213) ges-1p::GFP::daf-16) + rol-6(su1006)]. Pick Rollers to maintain.
CF1660 daf-16(mu86) I; daf-2(e1370) III; muIs84; muEx211. muIs84 [(pAD76) sod-3p::GFP + rol-6(su1006)]. muEx211 [ges-1p::daf-16::GFP + rol-6(su1006)]. Pick Rollers to maintain. Partial rescue of lifespan phenotype. Some animals show variable daf-16 expression in the intestine. Grows okay at 15C. [NOTE: muEx211 is quite unstable. Be sure to pick Rollers to avoid losing the array.]
CF1724 daf-16(mu86) I; daf-2(e1370) III; muIs105. muIs105 [daf-16p::GFP::daf-16 + rol-6(su1006)]. Rollers; Rol phenotype is not always evident). Integrated line derived from CF1449. Maintain at 15C. Forms dauers at 25C. Reference: Lin K, et al. Nat Genet. 2001 Jun;28(2):139-45.
CF1814 rrf-3(pk1426) II; daf-2(e1370) III. Daf-c at 25.5C; grow at 20C or less. Long lived.
CF1827 daf-16(mu86) I; daf-2(e1370) III; muEx268. muEx268 [ges-1p::GFP::daf-16(cDNA) + odr-1::RFP]. daf-16 GFP expressed in intestine. Partial rescue of lifespan phenotype. Grows okay at 15C. Pick RFP to maintain.
CF1844 rrf-3(b26) II; daf-2(mu150) III; fem-1(hc17) IV. Long lifespan. Maintain at 15C. Worms develop slowly at 20C, and are sterile at 25C. Reference: Garigan D, et al. Genetics. 2002 Jul;161(3):1101-12.
CF1980 rrf-3(pk1426) II; daf-2(e1368) III. Daf-c at 25.5C; grow at 20C or less. Long lived.
CF2005 daf-16(mu86) I; daf-2(e1370) III; muIs120. muIs120 [ges-1p::GFP::daf-16 + rol-6(su1006)]. Maintain at 15-20C. Rollers. Long-lived. Gamma irradiation-induced integration of muEx211. Rescues daf-16a1/c in the intestine (described in Libina et al, 2003). Reference: Zhang P, et al. Cell Metab, 2013. 17(1): p. 85-100.
CF2093 daf-16(mu86) I; daf-2(e1370) III; muIs131. muIs131 [unc-119p::GFP::daf-16 + rol-6(su1006)]. Maintain at 15-20C. Rollers. Modestly long-lived. Gamma irradiation-induced integration of muEx184. Rescues daf-16a1/c in neurons (described in Libina et al, 2003). Reference: Zhang P, et al. Cell Metab, 2013. 17(1): p. 85-100.
CF2102 daf-16(mu86) I; daf-2(e1370) III; muIs126. muIs126 [myo-3p::GFP::daf-16 + rol-6(su1006)]. Maintain at 15-20C. Rollers. Gamma irradiation-induced integration of muEx215. Rescues daf-16a1/c in muscles (described in Libina et al, 2003). Reference: Zhang P, et al. Cell Metab, 2013. 17(1): p. 85-100.
CF2570 daf-16(mu86) I; daf-2(e1370) III; muIs142. muIs142 [ges-1p::GFP::daf-16(cDNA) + odr-1p::RFP]. Maintain at 15-20C. Gamma irradiation-induced integration of muEx268. Rescues daf-16a1/c in the intestine (described in Libina et al, 2003). Reference: Zhang P, et al. Cell Metab, 2013. 17(1): p. 85-100.
CG499 daf-2(e1368) unc-103(n1213) III; him-5(e1490) V; rgEx178. rgEx178 [lev-11p::daf-2(+) + lev-11p::GFP]. rgEx178 rescues food deprivation suppression of unc-103(n1213)-induced spicule protraction.
CG500 daf-2(e1368) unc-103(n1213) III; him-5(e1490) V; rgEx180. rgEx180 [aex-3p::daf-2(+) + tnt-4p::GFP]. rgEx180 reduces unc-103(n1213)-induced spicule protraction, and rescues constitutive dauer formation.
CG640 daf-2(e1368) unc-103(n1213) III; him-5(e1490) V; rgEx247. rgEx247 [hsp-16p::daf-2(+) + lev-11p::GFP]. rgEx247 restores food-deprivation suppression of unc-103(n1213)-induced spicule protraction.
CS624 daf-2(e1370) III; lon-2(e678) X. Temperature-sensitive. Maintain at 15C. Lon. Daf-c.
DA438 bli-4(e937) I; rol-6(e187) II; daf-2(e1368) vab-7(e1562) III; unc-31(e928) IV; dpy-11(e224) V; lon-2(e678) X. Linkage mapping strain. Maintain at 15C.
DG2884 daf-2(e1370) III; fog-2(oz40) V. Male-female strain. Maintain by mating at 15 C.
DR128 dpy-1(e1) daf-2(e1370) III. Temperature sensitive dauer constitutive. Dpy. 100% dauers at 25C.
DR129 daf-2(e1370) unc-32(e189) III. Temperature senstive dauer constitutive. Unc-coiler. 100% dauers at 25C.
DR130 dpy-1(e1) daf-2(e1370) unc-32(e189) III. Temperature sensitive dauer constitutive. 100% dauers at 25C. Dpy. Unc-coiler. Reference strain for daf-2 complementation.
DR1309 daf-16(m26) I; daf-2(e1370) III.
DR1564 daf-2(m41) III. Temperature-sensitive dauer-consitutive, long-lived, intrinsically thermotolerant. Adults slightly shorter than adults of other daf-2 alleles. Up to 6% of L1s arrest at 25.5C. Makes nearly 100% dauers at 25C, 0% dauers at 15C. Good recovery of dauers at 15C.
DR1565 daf-2(m596) III. Class 2 allele of daf-2. Temperature sensitive Daf-c. Adults are long-lived (Age) and exhibit extrinsic thermotolerance (Itt).
DR1566 daf-2(m579) III. Adults Age and Itt and temperature sensitive Unc (class 2 allele). m579 results in an amino acid substitution(R437C) in the extracellular portion of the DAF-2 receptor (specifically, the Cys-rich domain).
DR1567 daf-2(m577) III. Class 1 allele of daf-2. Temperature sensitive Daf-c. Adults are long-lived (Age) and exhibit extrinsic thermotolerance (Itt).
DR1568 daf-2(e1371) III. Class 1 allele of daf-2. Temperature sensitive Daf-c. Adults are long-lived (Age) and exhibit extrinsic thermotolerance (Itt).
DR1572 daf-2(e1368) III. Maintain at 15C.  Class 1 allele of daf-2. Temperature sensitive Daf-c. Adults are long-lived (Age) and exhibit extrinsic thermotolerance (Itt).
DR1574 daf-2(e1391) III. Class 2 allele of daf-2. Temperature sensitive Daf-c. Adults are long-lived (Age) and exhibit extrinsic thermotolerance (Itt). Produces some dauers at 15C. CGC received new stock 10/25/00 from David Gems. Received new stock from Patrice Albert in the Riddle lab 6/04.
DR1942 daf-2(e979) III. This strain forms 20% dauers at 15C. At 25C there occurs about 25% embryonic arrest and about 75% L1 arrest. The e979 mutation results in an amino acid substitution, C146Y, in the ligand-binding domain of the DAF-2 receptor. [CGC received new stock of DR1942 September 2002. Previous stock was probably m41 and not e979.] [June 2004: Patrice Albert has confirmed the mutation in this stock: Repeat of sequencing for CGC collection strain DR1942 [daf-2(e979)] is complete. The strain does carry a C146Y mutation (coding strand TGC to TAC) [Mutation position is at 143, not 146, based on the amino acid sequence shown in Wormbase for daf-2. It's the C in partial sequence EKRCGPI of Exon 5.].]
DR200 daf-2(e1370) III; daf-13(m66) X. Temperature sensitive dauer constitutive. Dauers SDS-sensitive.
DR410 daf-2(m65)/unc-32(e189) III. Heterozygotes are WT and segregate WT, dauer constitutive, and Uncs. Lethal dauers.