| CB1364 |
daf-4(e1364) III. |
Temperature sensitive dauer constitutive. 100% dauers at 25C. Dauer recovery poor at 15C. Small. M-Mating--no success. |
| CB2196 |
daf-4(e1364) unc-32(e189) III. |
Temperature sensitive dauer constitutive. Unc. Closely linked. |
| CB2206 |
lon-1(e185) daf-4(e1364) unc-32(e189) III. |
Temperature sensitive dauer constitutive. Long. Unc. Linked closely. |
| DR508 |
unc-13(e450) I; daf-4(m72) III; sup-7(st5) X. |
sup-7 suppress dauer formation. sup-7 should be grown between 22.5C and 24C. |
| DR63 |
daf-4(m63) III. |
Temperature sensititve dauer constitutive. Small. 100% dauers at 25C. 15% dauers at 15C. |
| DR72 |
daf-4(m72) III. |
Temperature sensitive dauer constitutive. Small. Amber. Maintain at 15C. |
| OK66 |
daf-4(m72) III; cuIs2/+ IV. |
cuIs2 [myo-2c:: GFP + rol-6(su1006)]. daf-4(m72) is a temperature sensitive dauer constitutive mutant. Maintain at 15C. It was problematic to get cuIs2 homozygous in this background. Pick Rollers to maintain. |
| PJ105 |
cad-1(j1) II; daf-4(e1364) III. |
Very small, almost Dpy. Temperature sensitive dauer constitutive. Many dauers at 16C. Reduced cathepsin. |
| PJ1185 |
daf-5(e1386) II; daf-4(m592) III; ccIs55 V. |
ccIs55 [unc-54::lacZ + sup-7(st5)] V. Maintain at 15C. Small size and weak lacZ staining at 25C. daf-4 dauer defects are suppressed by daf-5, but not the small size. |
| PJ1228 |
daf-4(m592) III; ccIs55 V. |
ccIs55 [unc-54::lacZ + sup-7(st5)] V. Maintain at 15C. Leaky dauer constitutive -- ~95% dauer formation at 25C. Small body size at 20C. Strong lacZ staining in body wall at 15C; no staining at 25C. |
| PJ1257 |
daf-4(m592) III; unc-51(e369) ccIs55 V. |
ccIs55 [unc-54::lacZ + sup-7(st5)] V. Small. Unc. Most progeny shifted to 26C form dauers. |
| PJ1271 |
daf-4(m592) III; ccIs55 V; daf-3(e1376) X. |
ccIs55 [unc-54::lacZ + sup-7(st5)] V. Maintain at 15C. daf-4 is temperature-sensitive and dauer formation (not body size) phenotype is suppressed by daf-3. Dauers will form only when plates are crowded, starved, and maintained at 26C. Animals are small at 25C. |
| PJ1272 |
daf-4(m592) III; daf-18(e1375) IV; ccIs55 V. |
ccIs55 [unc-54::lacZ + sup-7(st5)] V. Abnormal-looking dauers form at 25C. |
| VC546 |
daf-4(ok827) III. |
C05D2.1. Temperature-sensitive dauer-constitutive, small. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807 |
| VC547 |
daf-4(ok828) III. |
C05D2.1. Temperature-sensitive dauer constitutive, small. Severe allele - most progeny are dauers at 20 degrees, and adults are more Dpy than Sma. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807 |