| CB1220 |
unc-82(e1220) IV. |
Slightly slow Unc. Movement almost wild-type. Body muscle abnormal. M-MATING++ 1-10%WT. |
| CB1323 |
unc-82(e1323) IV. |
Slow but normal development, disorganized muscle structure, somewhat Egl. |
| RW1350 |
unc-44(e362) unc-82(e1323)/stDf7 IV. |
Segregates heterozygotes that display only the unc-82 phenotype: slow but normal movement, disorganized muscle structure, somewhat Egl. stDf7 homozygotes die as embyros. unc-44 unc-82 homozygotes generally die as coiled, poorly-moving larvae. |
| RW3562 |
unc-44(e362) deb-1(st555)/unc-82(e1323) unc-24(e138) IV. |
Heterozygotes are WT and segregate WT, Uncs (unc-82 unc-24 homozygotes) and dead eggs (unc-44 deb-1 homozygotes). |
| RW3563 |
egl-19(st556)/unc-82(e1323) unc-24(e138) IV. |
Heterozygotes are WT and segregate WT, Uncs, and Pats. Maintain by picking WT and checking for correct segregation of progeny. st556 pka pat-5. |
| VC2535 |
unc-82(gk1124) IV. |
B0496.3. Identified by PCR, validated by CGH. External left primer: GATGTTGTCGCATTGTGTCC. External right primer: AACTTGATGGATCTGGTGGC. Internal left primer: TGCGCTTCTAATCGTAAGGC. Internal right primer: GGTTCCTCGTCAGGATCAAA. Internal WT amplicon: 2549 bp. Deletion size: 595 bp. Deletion left flank: AGAAACTAGACATAAATCAAGGTATTACTT. Deletion right flank: ACTAAAAGTAAAGGTTACAATTCCAAATTA. Insertion Sequence: AAATAGACATAAATCAAGGTATT. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807 |