Gene Information: che-3

Nameche-3 View on WormBase
Species C. elegans
SequenceF18C12.1
Genetic positionI:2.44 +/- 0.011 cM
Genomic positionI: 8062007..8081671

Strains carrying this gene

Strain Genotype Description
CB1124 che-3(e1124) I. Chemotaxis abnormal. Crawls off plate. Dauer defective. Small. M-MATING-NO SUCCESS.
CB1253 che-3(e1253) I. Chemosensory abnormal. Slightly small. Defective dauer formation. M-MATING NO SUCCESS.
CB1378 che-3(e1378) I. Dauer defective. Not leaky. Crowds. Amphid defect by EM. M-MATING-NO SUCCESS.
CB1379 che-3(e1379) I. Dauer defective. Crowds. Chemotaxis defective-Na+. M-MATING-NO SUCCESS.
CB450 unc-13(e450) che-3(ky1018) I. Paralyzed Unc. This strain carries a tightly linked mutation, che-3(ky1018), that changes Q1107 of che-3 to a stop codon. Reference: Larsch, et al. (2015) Cell Reports.
DR138 che-3(e1378) I; dpy-13(e184) IV. Dauer defective. Dpy.
DR139 che-3(e1379) I; dpy-13(e184) IV. Dauer defective. Dpy.
DR169 che-3(e1378) I; unc-5(e53) IV. Dauer defective. Unc-coiler.
DR292 che-3(e1379) I; him-8(e1489) IV. Dauer defective. Segregates males. Chemotaxis defective (Na+).
GOU2162 che-3(cas443[gfp::che-3]) I; xbx-1(cas502[xbx-1::tagRFP]) V. Constructed by crossing individual fluorescence knock-in worms. GFP inserted into the endogenous che-3 locus at the N-terminus and tagRFP::3xFlag inserted into the endogenous xbx-1 locus at the C-terminus by Cas9-triggered homologous recombination. Fluorescence enriched in most if not all sensory cilia. Very weak fluorescence in the cell bodies of ciliated neurons.
GOU2364 che-3(cas528[gfp::che-3(R1384C)]) I. GFP inserted into the endogenous che-3 gene at its N-terminus and R1384C mutation introduced by Cas9-triggered homologous recombination. Lipophilic dye filling is superficially wild-type and the amphid and phasmid sensory cilia are mildly shortened without evident IFT particle accumulation.
GOU2365 che-3(cas512[gfp::che-3(R1693C)]) I. GFP inserted into the endogenous che-3 gene at its N-terminus and R1693C mutation introduced by Cas9-triggered homologous recombination. Lipophilic dye filling is 100% defective and the amphid and phasmid sensory cilia are severely shortened without evident IFT particle accumulation.
GOU2366 che-3(cas511[gfp::che-3(K2935Q)]) I. GFP inserted into the endogenous che-3 locus at its N-terminus and K2935Q mutation introduced by Cas9-triggered homologous recombination. Lipophilic dye filling is 100% defective and the amphid and phasmid sensory cilia are severely shortened with strong IFT particle accumulation.
PH31 che-3(hf5) I. Resistant to caffeine. Previously called caf-2(hf5).
PH32 che-3(hf5) dpy-5(e61) I. Dpy. Resistant to caffeine. Previously called caf-2(hf5).
PR801 che-3(p801) I. Fails to avoid high osmotic strength solutions of NaCl and fructose. Fails to stain amphids with FITC.
VC1080 che-3(ok1574) I. F18C12.1. Superficially wild type. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
WU1055 che-3(am178) I. Ethosuximide resistant. Temperature sensitive dye-filling defective: WT dye-filling at 20C, dye-filling defective at 27C.