Gene Information: unc-53

Nameunc-53 View on WormBase
Species C. elegans
SequenceF45E10.1
Genetic positionII:3.17 +/- 0.006 cM
Genomic positionII: 11058047..11089456

Strains carrying this gene

Strain Genotype Description
CB404 unc-53(e404) II. Unc-cannot back. Males abnormal. Bursae abnormal. M-MATING-NO SUCCESS.
CB4567 unc-53(e2432) II. Unc-cannot back. Egl. Males abnormal. Multiple defects in neuronal outgrowth and branching, also defects in excretory canal extension and in sex muscles migration.
DG1255 unc-53(e404) sqt-1(e1350) II. e1350 is a recessive Dpy, dominant Roller. Unc.
MT152 unc-53(n152) II. Unc-cannot back. Egl. Males abnormal. Multiple defects in neuronal outgrowth and branching, also defects in excretory canal extension and in sex muscles migration. See also WBPaper00005353.
MT1580 dpy-10(e128) unc-53(n569) II. Dpy. Egl.
RB2073 unc-53(ok2736) II. ok2736. Homozygous. Outer Left Sequence: TTCCACAATCGAATGAACCA. Outer Right Sequence: TCAATTTCCGGATCTCAAGG. Inner Left Sequence: TCACGGGATCCATCGACA. Inner Right Sequence: AGCAAGAGCTCAAAGAACGC. Inner Primer PCR Length: 1282 bp. Deletion Size: 301 bp. Deletion left flank: TGCGGCACCGTGCATGAACATTCGGATTGT. Deletion right flank: GAGTTGCAGCCAGAGGATCGTTTCGAAGAT. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
ST13 klf-3(nc13)/dpy-10(e128) unc-53(n569) II; him-8(e1489) IV. Heterozygotes are WT and segregate WT, Dpy Uncs, and animals with muscle attachment defects and ventral cord displacement and detachment. Not well balanced. klf-3 was formerly known as mua-1.
ST30 spon-1(nc30) ncIs2/dpy-10(e128) unc-53(n569) II. ncIs2 [pH20::GFP + pBlueScript]. Heterozygotes are WT and segregate WT, DpyUnc, and animals with muscle attachment defects and ventral cord displacement and detachment which arrest in larval development. Not well balanced. Neurons visualized with ncIs2.
VC2036 unc-53(gk3156) II; gkDf26 V; hlh-19(gk1069) X. T04H1.1, F45E10.1, F57C12.3, T04H1.3, T04H1.2. The gk1069 allele was identified by PCR and validated by CGH, and can be detected with PCR using the following primers. External left primer: AAGGAACCTGCGGGATAACT. External right primer: CTTCCAGTAGGCAGTCAGGC. Internal left primer: CTGCTCCTCTTCCACGAGAC. Internal right primer: CCTTGTGTCCGAGTCCTCAT. Internal WT amplicon: 2234 bp. Deletion size: 716 bp. Deletion left flank: AATTGGTTTTTTTGATAAAGTTTGATTAGT. Deletion right flank: TTTCCAATATTTCATCAATTATTTGAACGC. Other lesions identified by CGH. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807