| CB1197 |
unc-44(e1197) IV. |
Coiler Unc. Growth slow. |
| CB1260 |
unc-44(e1260) IV. |
Paralyzed Unc. Small. |
| CB362 |
unc-44(e362) IV. |
Paralyzed Unc. Small. Recessive. M-MATING-NO SUCCESS. |
| CZ24990 |
unc-44(ju1412[unc-44::GFP]) IV. |
Endogenous unc-44 locus tagged for UNC-44L::GFP expression. The long isoform of UNC-44 is specific to neuronal expression. Reference: Chen F, Chisholm AD, Jin Y. Development. 2017 Feb 15;144(4):698-707. |
| CZ25013 |
unc-44(ju1413[unc-44::gfp::LoxP::3xflag]) IV. |
unc-44(ju1413[unc-44::GFP::LoxP::3xflag]) IV. UNC-44C (short isoform of UNC-44) tagged with GFP. UNC-44C is strongly expressed in multiple tissues: nervous system (from 1.5-fold stage to adult), epidermis (from early embryo to adult), seam cells (from L1 to L4), vulva (from L3 to adult), and spermatheca/sheath cells (from L4 to adult). Reference: Chen F, Chisholm AD, Jin Y. Development. 2017 Feb 15;144(4):698-707. |
| DR1213 |
unc-44(e362) unc-24(e138) IV. |
Unc-44 masks the Unc-24 phenotype. Paralyzed coiler, Dpy, tends to curl. |
| EH135 |
unc-44(e362) bli-6(sc16) IV. |
Blistered. Unc. |
| MT5241 |
unc-5(e53) unc-44(e362) IV. |
Unc. |
| RW1350 |
unc-44(e362) unc-82(e1323)/stDf7 IV. |
Segregates heterozygotes that display only the unc-82 phenotype: slow but normal movement, disorganized muscle structure, somewhat Egl. stDf7 homozygotes die as embyros. unc-44 unc-82 homozygotes generally die as coiled, poorly-moving larvae. |
| RW3562 |
unc-44(e362) deb-1(st555)/unc-82(e1323) unc-24(e138) IV. |
Heterozygotes are WT and segregate WT, Uncs (unc-82 unc-24 homozygotes) and dead eggs (unc-44 deb-1 homozygotes). |
| STR237 |
unc-44(hrt2) IV. |
Severely Unc. CRISPR-generated deletion removes 111 bp (ACGATAAGAAAACTA...ATGAATCCGCCCAAG). hrt2 allele is specific to the AO13 unc-44 isoform. Reference: He L, et al. eLife 2020;9:e55111 doi: 10.7554/eLife.55111. |
| STR282 |
unc-44(hrt5[unc-44::GFP]) IV. |
CRISPR/Cas9-engineered insertion of GFP tag into the large AO13 splice isoform of the endogenous unc-44 locus at amino acid 6303. Slightly reduced body bends while crawling. Reference: He L, et al. eLife 2020;9:e55111 doi: 10.7554/eLife.55111. |
| SU62 |
unc-44(e1260) lag-1(q385)/zen-4(w35) IV. |
Heterozygotes are WT and segregate WT, Unc L1 lethals and dead eggs (homozygous zen-4(w35)). |