Gene Information: rol-6

Namerol-6 View on WormBase
Species C. elegans
Genetic positionII:0.87 +/- 0.002 cM
Genomic positionII: 8733050..8734239

Strains carrying this gene

Strain Genotype Description
AG226 rol-6(e187) unc-4 (e120)/mnC1 [dpy-10(e128) unc-52(e444) nIs190 let-?] II; him-8(e1489) IV. nIs190 [myo-2::GFP]. Him. Heterozygotes are wild-type GFP+ and segregate WT GFP+ heterozygotes, Rol Uncs, dead embryos, and males. nIs190 [myo-2::GFP] integrated in or near mnC1. Approx 0.5% recombination seen between nIs190 and mnC1. Fails to complemement all markers on mnC1.
CB187 rol-6(e187) II. Right hand Roller. Recessive. M-MATING-NO SUCCESS.
CF12 rol-6(e187) II; lin-22(n372) IV; him-5(e1490) V. Rollers. lin-22 and him-5 mutations affect neuroblast formation from epidermal precursor cell V5.
CL802 smg-1(cc546) I; rol-6(su1006) II. Rollers. Maintain under normal conditions. Standard control for CL4176; originally used CL1175 as the control, but subsequently it was found that CL1175 can produce some A-Beta. Reference: Fonte V., et al. Mol Neurodegener. 2011 Aug 23;6(1):61. [NOTE: The temperature-sensitive allele cc546 causes an M1957L change in SMG-1. The lesion is an atg>ttg transversion in exon 35. Flanking sequences follow with the mutation site indicated with a capital A: ttggtggtcggttacaaaacgatattcaaga tcactggcagtcatgagtAtggttggatcagttttaggactcggtgatcg acatttggacaatttattg The lesion is detectable via SNP-snip with the mutation causing loss of an MslI site. Primers are for a 323 bp product. Digest with MslI to 86+237 in the wild type, uncut as 323 in the mutant. DJR701(f): CAGTCGTGAGCTTTGGATGCGTGC DJR702(r): TCGGGGATACGCAGATTCTTTCCC. Pedone ... Reiner G3 (2021).]
CX3940 kyIs140 I; rol-6(e187) II; slo-1(ky399) V. kyIs140 [str-2::GFP + lin-15(+)] I. In ky399 mutants str-2::GFP is expressed in both AWX neurons. ky399 is a semi-dominant allele of slo-1, a large conductance potassium channel. PKA nsy-3(ky399). Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
DA438 bli-4(e937) I; rol-6(e187) II; daf-2(e1368) vab-7(e1562) III; unc-31(e928) IV; dpy-11(e224) V; lon-2(e678) X. Linkage mapping strain. Maintain at 15C.
DR518 rol-6(su1006) unc-4(e120) II. RollerUnc.
EG1000 dpy-5(e61) I; rol-6(e187) II; lon-1(e1820) III. Dpy suppresses Rol and Lon. Strain appears to be only Dpy. Useful for mapping, especially Unc mutations. Separately, dpy-5 causes extreme dumpiness, rol-6 causes worms to roll over and lie in a "C" shape, and lon-1 worms are about 125% WT length.
EW23 unc-104(e1265) rol-6(e187) II. Dpy. Roller.
GD211 rol-6(e187) hlh-6(tm299) unc-4(e120) II.
GE1710 rol-6(e187) unc-4(e120) II. Roller Unc.
GE1712 vab-9(e1744) rol-6(e187) unc-4(e120) II. Unc. Roller. Tail whip knobbed at all stages except adult male (adult male tail tip slightly swollen).
HE1006 rol-6(su1006) II. Dominant Roller.
JJ1550 dpl-1(zu355) unc-4(e120)/rol-6(e187) let-23(sy97) II. Heterozygotes are WT and segregate WT, Uncs which give only deads egss with a Mex phenotype, and Vulvaless Rollers. sy97 is only 15% viable.
JK3091 ehn-1(q690) rol-6(e187) II. Rollers. About 15% have a one-armed gonad or are WP. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
JW29 jeIs1I. jeIs1 [mec-7(+)::lacZ + rol-6(su1006)]. Rollers. Males will mate.
MDH10 ast-1(hd1) rol-6(e187) II; ceh-43(ot406) III; vtIs1 V. vtIs1 [dat-1p::GFP + rol-6(su1006)] V. Rollers. ot406 has a dopaminergic phenotype.
MDH91 ast-1(hd1) rol-6(e187) II; ceh-20(ok541) III; vtIs1 V; ceh-40(gk159) X; muEx261. vtIs1 [dat-1p::GFP + rol-6(su1006)] V. muEx261 [ceh-20::GFP + odr-1::RFP]. Pick RFP+ animals to maintain. Rollers. Embryonic lethality of ceh-20(ok541); ceh-40(gk159) double mutants is rescued by muEx261.
ML503 tra-2(q276)/rol-6(e187) II; mcDf2/dpy-8(e130) X. Segregates WT, males, Dpy, Rol, DpyRol, dead eggs (mcDf2). Use XX tra-2 males to move mcDf2 around. mcDf2 is an approx. 500 kb deficiency removing a large chunk between mec-2 and sup-7 (non-inclusive).
MT2709 rol-6(e187n1270) II. Revertant. Phenotypically WT.
MT3751 dpy-5(e61) I; rol-6(e187) II; unc-32(e189) III.
OH2724 otIs133; otEx1545. otIs133 [pttx-3::RFP + unc-4(+)]. otEx1545 [F11H8.2::GFP + rol-6(su1006)]. Maintain by picking GFP+ Rollers. RFP expressed in AIY only. Reference: Wenick AS, Hobert O. Wenick AS, Hobert O. Developmental Cell. 2004 Jun;6(6):757-70.
OH7317 F21H12.1(ot86) rol-6(e187) II; ntIs1 V. ntIs1 [gcy-5p::GFP + lin-15(+)] V. Ectopic expression of ntIs1 in ASEL. Rollers. Whole genome sequenced strain.
WM79 rol-6(n1270) II; neEx1. Rollers. n1270 is phenotypically wild type. neEx1[LIT-1::GFP + rol-6(su1006)]. LIT-1::GFP has full length LIT-1 fused to GFP in a YAC. Pick Rollers to maintain.