Gene Information: unc-14

Nameunc-14 View on WormBase
Species C. elegans
SequenceK10D3.2
Genetic positionI:1.79 +/- 0.003 cM
Genomic positionI: 7122614..7128246

Strains carrying this gene

Strain Genotype Description
CB57 unc-14(e57) I. Unc.
CGC140 goa-1(n499)/tmC20 [unc-14(tmIs1219) dpy-5(tm9715)] I. Homozygous lethal mutation balanced by Dpy- and myo-2p::Venus-marked inversion. Heterozygotes are paralyzed Unc and Egl with relatively dim pharyngeal GFP (Venus) expression. Heterozygotes segregate heterozygous non-Dpy GFP+ paralyzed Unc and Egl, non-GFP embryonic lethal (homozygous n499), and Dpy with brighter GFP+ (tmC20 homozygous). This strain is difficult and time consuming to maintain. Gives relatively few heterozygotes. Remove Dpy from plate to prevent them from taking over. Heterozygotes tend to stack up in parallel clumps. Populations can be enriched by transferring these clumps to new plates and allowing Dpy (tmC20 homozygotes) to crawl out into bacterial lawn, and then picking away Dpy or transferring the clump of Hets to another plate. Derived by balancing n499 from parental strain MT1102 over tmC20 from FX30179.
DR436 dpy-5(e61) unc-14(e57) I. Dpy. Unc.
FX11501 dpy-5(e61) uri-1(tm939)/dpy-5(e61) unc-14(e57) I. Heterozygotes are Dpy. Segregate Dpy Unc. tm939 homozygotes are emb, leth, pvl, rup, larval arrested or sterile. Attribution: This strain was generated by the National Bioresource Project at the Tokyo Women's Medical University School of Medicine, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
FX30177 tmC20 [unc-14(tmIs1219)] I. Break points: In(F53G12.8 T02E1.7 In(gsp-3 sre-23)) I. Covered region (Mb) 8.1 (0.1..8.3) Balancer marked with myo-2p::Venus. tmIs1219 is inserted in unc-14, but Unc phenotype is not detectable. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241.
FX30179 tmC20 [unc-14(tmIs1219) dpy-5(tm9715)] I. Break points: In(F53G12.8 T02E1.7 In(gsp-3 sre-23)) I. Covered region (Mb) 8.1 (0.1..8.3) Balancer marked with myo-2p::Venus. Dpy. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241.
MT6356 dpy-5(e61) nDf43/dpy-14(e188) unc-14(e57) I. Heterozygotes are WT and segregate WT, DpyUncs and animals which arrest as L1 or L2 larvae (nDf43) homozygotes.
NJ341 unc-14(rh106) I. Reduced PVP staining, Unc.
NJ365 unc-14(rh111) I. PVP varicosities, amphid -, phasmid - , CEP+.