CB57 |
unc-14(e57) I. |
Unc. |
CGC140 |
goa-1(n499)/tmC20 [unc-14(tmIs1219) dpy-5(tm9715)] I. |
This strain is difficult and time consuming to maintain. Gives relatively few heterozygotes. Homozygous lethal mutation balanced by Dpy- and myo-2p::Venus-marked inversion. Heterozygotes are paralyzed Unc and Egl with relatively dim pharyngeal GFP (Venus) expression. Heterozygotes segregate heterozygous non-Dpy GFP+ paralyzed Unc and Egl, non-GFP embryonic lethal (homozygous n499), and Dpy with brighter GFP+ (tmC20 homozygous). Remove Dpy from plate to prevent them from taking over. Heterozygotes tend to stack up in parallel clumps. Populations can be enriched by transferring these clumps to new plates and allowing Dpy (tmC20 homozygotes) to crawl out into bacterial lawn, and then picking away Dpy or transferring the clump of Hets to another plate. Derived by balancing n499 from parental strain MT1102 over tmC20 from FX30179. |
DR436 |
dpy-5(e61) unc-14(e57) I. |
Dpy. Unc. |
FX11501 |
dpy-5(e61) uri-1(tm939)/dpy-5(e61) unc-14(e57) I. |
Heterozygotes are Dpy. Segregate Dpy Unc. tm939 homozygotes are emb, leth, pvl, rup, larval arrested or sterile. Attribution: This strain was generated by the National Bioresource Project at the Tokyo Women's Medical University School of Medicine, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807 |
FX30177 |
tmC20 [unc-14(tmIs1219)] I. |
Break points: In(F53G12.8 T02E1.7 In(gsp-3 sre-23)) I. Covered region (Mb) 8.1 (0.1..8.3) Balancer marked with myo-2p::Venus. tmIs1219 is inserted in unc-14, but Unc phenotype is not detectable. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. |
FX30179 |
tmC20 [unc-14(tmIs1219) dpy-5(tm9715)] I. |
Break points: In(F53G12.8 T02E1.7 In(gsp-3 sre-23)) I. Covered region (Mb) 8.1 (0.1..8.3) Balancer marked with myo-2p::Venus. Dpy. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. |
MT6356 |
dpy-5(e61) nDf43/dpy-14(e188) unc-14(e57) I. |
Heterozygotes are WT and segregate WT, DpyUncs and animals which arrest as L1 or L2 larvae (nDf43) homozygotes. |
NJ341 |
unc-14(rh106) I. |
Reduced PVP staining, Unc. |
NJ365 |
unc-14(rh111) I. |
PVP varicosities, amphid -, phasmid - , CEP+. |