| BS509 |
ozDf2/dpy-21(e428) par-4(it33) V. |
Heterozygotes are wild-type and segregate wild-type (heterozygotes), Dpys (dpy-21 par-4 homozygotes that lay dead eggs), and dead eggs (ozDf2 homozygotes). Maintain by picking wild-type. |
| KK184 |
par-4(it47) V. |
Strict maternal effect lethal. Temperature sensitive-grow at 15C. Par at 20C and 25C. |
| KK300 |
par-4(it57) V. |
Temperature-sensitive maternal effect lethal (strict). Maintain at 15-16C (95% of embyros hatch). Shift to 25C for Par phenotype (no hatching embryos). |
| UTR43 |
par-4(nar12[par-4Ap::mNG + loxP sqt-1(gf) Hygromycin(+) loxP 3X FLAG]) V. |
Homozygous viable, Rol. nar12 (non-excised strain) is null for par-4A & C, but not for par-4B, and is viable. Low rate of spontaneous excision even when maintained at 15C. Pick Rollers to maintain. Reference: Roy et al. microPublication Biology. https://www.micropublication.org/roy_2018_mngpar-4.html |
| UTR45 |
par-4(nar13[mNG::3X FLAG::par-4a + loxP]) V. |
Superficially wild-type. nar13 was derived by excision of UTR43(nar12) cassette by heat shock. Tagged endogenous PAR-4A & C: weak, ubiquitous fluorescence. Reference: Roy et al. microPublication Biology. https://www.micropublication.org/roy_2018_mngpar-4.html |