| EU459 |
unc-13(e1091) mom-5(zu193)/hT2 I; +/hT2 [bli-4(e937) let-?(h661)] III; him-8(ec56) IV. |
Heterozygotes are WT and segregate WT and Uncs. The Uncs have a non-conditional maternal-effect embryonic lethal phenotype: the E blastomere adopts MS fate in about 5% of mutant embryos. The Uncs are 100% penetrant for embyronic lethality and morphogenesis defects. |
| EU550 |
div-1(or148) III; him-8(e1489) IV. |
Temperature sensitive. Maintain at 15C. |
| EU588 |
him-8(e1489) IV; spn-4(or191) V. |
Temperature sensitive. Viable at 15C; lethal at 25C. At 25C, embryos exhibit defects in spindle orientation and cell fate patterning. Throws males. |
| EU603 |
lit-1(or131) III; him-8(e1489) IV. |
or131 mutant embyros have a highly penetrant mom-like defect with mutant embryos lacking gut and making extra pharyngeal tissue at 25C. Maintain at 15C. |
| EU641 |
cul-2(or209) III; him-8(e1489) IV. |
Temperature sensitive. Maintain at 15C. |
| EU780 |
spd-2(or293) I; him-8(e1489) IV. |
Him. Temperature-sensitive embryonic lethal. Maintain at 15C. Reference: O'Rourke SM, et al. PLoS One. 2011 Mar 1;6(3):e16644. |
| EU863 |
div-(or361) him-8(e1489) IV. |
Temperature sensitive. Maintain at 15C. |
| EU869 |
div-(or389) II; him-8(e1489) IV. |
Temperature sensitive. Maintain at 15C. |
| EU871 |
div-(or235) III; him-8(e1489) IV. |
Temperature sensitive. Maintain at 15C. |
| EU872 |
div-(or366) I; him-8(e1489) IV. |
Temperature sensitive. Maintain at 15C. |
| EU878 |
div-(or335) I; him-8(e1489) IV. |
Temperature sensitive. Maintain at 15C. |
| EU879 |
div-1(or345) III; him-8(e1489) IV. |
Temperature sensitive. Maintain at 15C. |
| EU882 |
div-(or310) I; him-8(e1489) IV. |
Temperature sensitive. Maintain at 15C. |
| EU883 |
div-(or364) III; him-8(e1489) IV. |
Temperature sensitive. Maintain at 15C. |
| EU920 |
lit-1(or393) III; him-8(e1489) IV. |
Temperature-sensitive embryonic lethal. Maintain at 15C. Reference: O'Rourke SM, et al. PLoS One. 2011 Mar 1;6(3):e16644. |
| JJ1600 |
par-3(it71) lon-1(e185) III; him-8(e1489) IV; zuIs20. |
zuIs20 [par-3p::par-3::ZF1::GFP + unc-119(+)]. Lon. Him. GFP-tagged PAR-3 that is degraded in early embryonic somatic cells. Rescues the Par phenotype of par-3(it71). Delayed endodermal cell gastrulation. Reference: Nance J, Munro EM, Priess JR. Development. 2003 Nov;130(22):5339-50. |
| JJ1743 |
par-6(tm1425)/hIn1 [unc-54(h1040)] I; him-8(e1489) IV. |
Heterozygotes are WT and segregate WT, Uncs, dead larvae (par-6 homozygotes) and males. |
| JK1499 |
mog-2(q75)/sqt-1(e1350) II; him-8(e1489) IV. |
At 15C heterozygotes are Rollers and segregate Rollers, Sqt and Steriles. Not a healthy strain; recombination occurs, too. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. |
| JR8 |
die-1(e2500)/mnC1 [dpy-10(e128) unc-52(e444)] II; him-8(e1489) IV. |
Heterozygotes are WT and segregate WT, paralyzed Dpys, and arrested embryos. Throws males. e2500 pka zen-2(e2500). |
| JUP1 |
oxSi120 II; him-8(e1489) IV. |
oxSi120 [peel-1p::tagRFP::MSP-142 3'utr+ unc-119(+)]. Him. Derived from EG5897 and CB1489; not known if is unc-119(ed3) is still present in background. Reference: Batchelder EL, et al. Proc Natl Acad Sci U S A. 2011 Jul 12;108(28):11429-34. |
| LB138 |
him-8(e1489) IV; uaDf5/+. |
uaDf5/+ is stable as a heterozygote. The strain does not appear to segregate live animals with uaDf5/uaDf5 or +/+ genotypes. Throws males. |
| LB90 |
ctl-2(ua90) II; him-8(e1489) IV. |
Shortened lifespan. |
| LC141 |
him-8(e1489) IV; cat-4(e3015) V. |
Reduction in serotonin and dopamine, most apparent in young larva; bleach hypersensitivity and cuticle fragility in adult, but less than null mutants. Derived by outcrossing CB7107 six times to N2. Reference: Loer CM, et al. Genetics. 2015 May;200(1):237-53. |
| NA404 |
him-8(e1489) qui-1(gb404) IV. |
Quinine avoidance defective. In qui-1(gb404) a CAA to TAA transition at position 11215 of Y45F10B generates a stop codon in the fifth exon of Y45F10B.10. Putative null allele. |
| NA43 |
gus-1(b410gb173) I; him-8(e1489) IV. |
Intragenic revertant restoring to almost WT level of b-glucuronidase activity. Throws males. |
| NM5187 |
jsTi1453 I; him-8(e1489) IV. |
jsTi1453 [LoxP::rpl-28p::FRT::GFP::his-58::FRT3] I. jsTi1453 is an RMCE landing site inserted using miniMos located on Chr I at 11,933,068 (at 13.1 m.u.) between R06C1.4 and R06C1.6. Insertion site ccctactatcaacgcaaaaactatttggcttttactTAaacataacgttttgaatttgaaaatcaaaaag with rpl-28p trancription toward R06C1.4. Reference: Nonet ML. Genetics. 2020. |
| NM5209 |
jsTi1453 jsSi1514 I; him-8(e1489) IV. |
jsTi1453 [LoxP::rpl-28p::FRT::GFP::his-58::FRT3] I. jsSi1514 [LoxP::RMCE mec-4p::GFP-C1::FRT3] I. RMCE insertion of mec-4 promoter driving GFP-C1. Reference: Nonet ML. Genetics. 2020. |
| OE3001 |
him-8(e1489) IV; dyf-4(m158) V. |
Defective in dye filling (FITC or DiO) of amphid and phasmid neurons. Chemotaxis defective. Throws males. |
| OE3002 |
him-8(e1489) IV; xbx-1(ok279) V. |
Dyf. Osm. Throws males. Reduced mating efficiency (ME 2-3). Deletion extends over 1610 bp in the intron between exons 3 and 4 and ending 30 bp after the STOP codon (cosmid F02D8 pb 25954-27563 are deleted). Complements dyf-4(m158). |
| OH10194 |
ceh-43(tm480) otIs221 III; him-8(e1489) IV; norEx41. |
otIs221 [cat-1::GFP]. norEx41 [ceh-43(fosmid) + dat-1::mCherry]. Him. tm480 embryonic lethality is rescued by extrachromosomal array. Pick mCherry+ animals to maintain. |
| OH10195 |
ceh-43(tm480) III; him-8(e1489) IV; otIs225; norEx41. |
otIs225 [cat-4::GFP]. norEx41 [ceh-43 (fosmid) + dat-1::mCherry]. Him. tm480 embryonic lethality is rescued by extrachromosomal array. Pick mCherry+ animals to maintain. |
| OH11054 |
pha-1(e2123) III; him-8(e1489) IV; ntIs1 V; otIs305; otEx4445. |
ntIs1 [gcy-5p::GFP + lin-15(+)] V. otIs305 [hsp-16.2p::che-1::3xHA::BLRP + rol-6(su1006)]. otEx4445 [snb-1::NLS::RFP + pBX]. Rollers. Maintain by picking RFP+ Rollers. Maintain at 20C to maintain extrachromosomal array. Reference: Tursun B, et al. Science. 2011 Jan 21;331(6015):304-8. |
| OH15733 |
dmd-3(ot932[dmd-3::GFP::3xFlag]); him-8 (e1489) IV. |
GFP and 3xFlag tag inserted in endogenous dmd-3 locus using the CRISPR SEC cassette (Dickinson 2015). sgRNA: accctttcagccgtattgt Insertion site: V: 19650564-19650565. Reference: Pereira L, et al. Elife. 2019 Jan 1;8. pii: e42078. doi: 10.7554/eLife.42078. |
| OH16298 |
him-8(e1489) IV; otIs670 V. |
Him. [NOTE: strain does not segregate many male progeny.] otIs670 provides a healthier alternative to otIs669, performing better in a variety of phenotypic assays. otIs670 [UPN::NLS::TagRFP-T + acr-5::NLS::mTagBFP2::H2B + flp-1::NLS::mTagBFP2::H2B + flp-6::NLS::mTagBFP2::H2B + flp-18::NLS::mTagBFP2::H2B + flp-19::NLS::mTagBFP2::H2B + flp-26::NLS::mTagBFP2::H2B + gcy-18::NLS::mTagBFP2::H2B + ggr-3::NLS::mTagBFP2::H2B + lim-4::NLS::mTagBFP2::H2B + pdfr-1::NLS::mTagBFP2::H2B + srab-20::NLS::mTagBFP2::H2B + unc-25::NLS::mTagBFP2::H2B + cho-1::NLS::CyOFP1::H2B + flp-13::NLS::CyOFP1::H2B + flp-20::NLS::CyOFP1::H2B + gcy-36::NLS::CyOFP1::H2B + gpa-1::NLS::CyOFP1::H2B + nlp-12::NLS::CyOFP1::H2B + nmr-1::NLS::CyOFP1::H2B + ocr-1::NLS::CyOFP1::H2B + osm-9::NLS::CyOFP1::H2B + srh-79::NLS::CyOFP1::H2B + sri-1::NLS::CyOFP1::H2B + srsx-3::NLS::CyOFP1::H2B + unc-8::NLS::CyOFP1::H2B + acr-2::NLS::mNeptune2.5 + ceh-2::NLS::mNeptune2.5 + dat-1::NLS::mNeptune2.5 + dhc-3::NLS::mNeptune2.5 + eat-4::NLS::mNeptune2.5 + flp-3::NLS::mNeptune2.5 + gcy-35::NLS::mNeptune2.5 + glr-1::NLS::mNeptune2.5 + gcy-21::NLS::CyOFP1::H2B::T2A::NLS::mTagBFP2::H2B + klp-6::NLS::mNeptune2.5::T2A::NLS::CyOFP1::H2B + lim-6::NLS::mNeptune2.5::T2A::NLS::CyOFP1::H2B + mbr-1::NLS::mNeptune2.5::T2A::NLS::mTagBFP2::H2B + mec-3::NLS::CyOFP1::H2B::T2A::NLS::mTagBFP2::H2B + odr-1::NLS::mNeptune2.5::T2A::NLS::mTagBFP2::H2B + srab-20::NLS::mNeptune2.5::T2A::NLS::mTagBFP2::H2B] V. UPN (Ultra Pan-Neuronal) promoter contains four short pan-neuronal promoters fused together (unc-11::rgef-1::ehs-1::ric-19). NeuroPAL (Neuronal Polychromatic Atlas of Landmarks) transgene used to resolve unique neural identities in whole-brain images. Reference: Yemini E, et al. Cell. 2021 Jan 7;184(1):272-288.e11. PMID: 33378642 Free pre-print available at https://www.biorxiv.org/content/10.1101/676312v2 |
| OH4605 |
unc-37(ot59)/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III); him-8(e1489) IV; otIs3 V. |
Heterozygotes are WT and GFP+ in the pharynx. ot59 is homozygous inviable. qIs48 is an insertion of ccEx9747 (carries myo-2::GFP, pes-10::GFP, and a gut enhancer fused to GFP) onto the hT2 chromosome and is homozygous lethal. otIs3[lin-15(+) + gcy-7::GFP]. otIs3 is expressed in ASEL in WT animals. In this ot59 strain, otIs3 is expressed in ASEL and ASER. |
| OH7193 |
otIs181 III; him-8(e1489) IV. |
otIs181 [dat-1::mCherry + ttx-3::mCherry]. Him. Expression in DA neurons (ADE, PDE, CEP, male tail) and AIY. Reference: Flames N, Hobert O, 2009 Nature 458, 885-889. |
| OH8480 |
otIs221 III; him-8(e1489) IV. |
otIs221 [cat-1::GFP]. GFP expression in monoaminergic neurons. Reference: Flames N, Hobert O, 2009 Nature 458, 885-889. |
| OH9639 |
ntIs1 V; him-8 IV; lim-6(ot146) X. |
ntIs1 [gcy-5p::GFP + lin-15(+)] V. 2 ASER. Him. |
| PB192 |
Cbr-him-8(v188) I; stIs20120 X. |
stIs20120 [Cbr-myo-2p::GFP + Cbr-unc-119(+)] X. Him. Reference: Ragavapuram V, et al. G3 (Bethesda). 2015 Dec 31. |
| PJ1022 |
him-8(e1489) IV; ccIs55 V. |
ccIs55 [unc-54::lacZ + sup-7(st5)] V. |
| PJ1036 |
unc-38(e264) I; him-8(e1489) IV; ccIs55 V. |
ccIs55 [unc-54::lacZ + sup-7(st5)] V. Him. Resistant to 1 mM levamisole. |
| SM1480 |
mxl-2(tm1516) pha-1(e2123) III; him-8(e1489) IV. |
Worms are viable at 15C and dead at 24C. |
| SP1109 |
him-8(mn253) IV. |
|
| SP1784 |
dpy-1(e1) ncl-1(e1865) unc-36(e251) III; him-8(e1489) IV; mnDp90 [dpy-1(+) ncl-1(+) unc-36(+) unc-93(+)] (III;f). |
Animals with mnDp90 are WT. Animals which have lost mnDp90 are DpyUnc and Ncl. Throws males. Males containing mnDp90 are fertile. mnDp90 was derived from mnDp86. |
| ST13 |
klf-3(nc13)/dpy-10(e128) unc-53(n569) II; him-8(e1489) IV. |
Heterozygotes are WT and segregate WT, Dpy Uncs, and animals with muscle attachment defects and ventral cord displacement and detachment. Not well balanced. klf-3 was formerly known as mua-1. |
| TN145 |
him-8(e1489) IV; adt-1(cn30) X. |
Morphological changes in the rays, especially transformation of ray 6 into a thickened shape. Appearance of abnormal protuberances around rays. Closed structure of the fan. Impaired mating ability. Exons 8-10 of the adt-1 gene, encoding most of the metalloproteinase domain of ADT-1, are deleted in adt-1(cn30). |
| TY1775 |
yIs2 him-8(e1489) IV. |
yIs2 [xol-1::laxZ + rol-6(su1006)] IV. Rollers. XO-specific expression of lacZ fusion. |
| TY2071 |
him-8(e1489) IV; dpy-3(e27) unc-2(e55) X; yDp16 (X;f). |
non-Unc, somewhat Dpy hermaphrodites. Gives DpyUncs when yDp16 is lost. |
| TY2139 |
mnDp66 (X;I)/yDp14 (X;I); him-8(e1489) IV; yDf13 unc-1(e1598n1201) dpy-3(e27) X. |
Heterozygotes are WT hermphrodites whose progeny include WT hermaphrodites, Dpy hermaphrodites (mnDp66; yDf13 unc-1 dpy-3), WT males and Dpy males. |
| TY2173 |
mnDp66 (X;I)/yDp14 (X;I); him-8(e1489) IV; yDf17 X. |
The hermaphrodites are variable in phenotype, but most are Dpyish (small) and sick. Pick these hermaphrodites to maintain the strain, since healthier animals may have picked up a suppressor mutation or gone polyploid, etc. Strain gives WT males. |