Gene Information: him-8

Namehim-8 View on WormBase
Species C. elegans
SequenceT07G12.12
Genetic positionIV:4.64 +/- 0.000 cM
Genomic positionIV: 10561905..10563974

Strains carrying this gene

Strain Genotype Description
AG226 rol-6(e187) unc-4 (e120)/mnC1 [dpy-10(e128) unc-52(e444) nIs190 let-?] II; him-8(e1489) IV. nIs190 [myo-2::GFP]. Him. Heterozygotes are wild-type GFP+ and segregate WT GFP+ heterozygotes, Rol Uncs, dead embryos, and males. nIs190 [myo-2::GFP] integrated in or near mnC1. Approx 0.5% recombination seen between nIs190 and mnC1. Fails to complemement all markers on mnC1.
BL5717 inIs179 II; him-8(e1489) IV. inIs179 [ida-1p::GFP]. GFP is expressed in a subset of neurons and the neuroendocrine uv1 cells of the vulva. Identified neurons include ADE, ALA, ASI, ASK, AUA, ASG, AVH, AVJ, AVK, VC, HSN, PDE, PVP, PHA, PHB, PHC. Male sex-specific neurons include CA and some ray neurons. Plasmid pida-1::GFP 7.6 was injected into N2 and integrated to generate BL5715, then crossed into him-8(e1489) background.
BW1199 him-8(e1489) IV; unc-42(e270) sma-1(e30) V; ctDp8[vab-8(e1017)] (V;f).
BW1434 dpy-17(e164) III; him-8(e1489) IV; her-1(y101hv1) unc-42(e270) V; ctDp11 (III;V;f). Wild type hermaphrodites and males which produce WT, Dpy, Unc and DpyUnc progeny due to mating or loss of the duplication. Pick WT hermaphrodites and males to maintain.
CA151 him-8(me4) IV. 40% XO self progeny. Recessive.
CA257 him-8(tm611) IV. 37% XO self progeny. Recessive.
CB1489 him-8(e1489) IV. 37% XO self-progeny. 6% XXX self-progeny. Recessive. M-MATING++ 1-10%WT. [Background mutation that causes rays 8 and 9 to fuse.]
CB4017 fem-1(hc17) him-8(e1489) IV; unc-1(e1598) X. Severely Uncoordinated; Him; feminized at temperatures above 20C. Grow at 15C. Used for generating patroclinous WT males, by exploiting non-disjunction caused by him-8, and dominant sex-linked unc-1.
CB4355 him-8(e1489) IV; smg-4(ma116) V. Hermaphrodites have an abnormal protrusive vulva. Segregate males with abnormally swollen bursa. ma116 is a weak recessive suppressor of certain alleles of certain genes.
CB4689 dpy-28(y1) III; him-8(e1489) IV. y1 is a temperature sensitive mutation affecting only XX animals. him-8(e1489) causes X non-disjunction, resulting in many males. Grow at 15C. XX and XXX animals not viable above 15C. After shifting to 25C, surviving population is almost all XO males.
CB4951 spe-12(hc76) I; him-8(e1489) IV. Male/female strain which must be maintained by mating. spe-12(hc76) prevents self-fertility in hermaphrodites but does not impair spermatogenesis in males. Presence of him-8(e1489) increases male frequency.
CB5281 air-2(e2631)/dpy-5(e61) I; him-8(e1489) IV. WT hermaphrodites segregate WT hermaphrodites and males, Dpy hermaphrodites and males and Stu (Sterile and Uncoordinated) hermaphrodites and males.
CB5311 egl-26(e1952e2650) II; him-8(e1489) IV. Partial intragenic revertant. Weak Egl phenotype, less severe than egl-26(e1952). Reference: Hodgkin (1986) PMID: 3770465.
CB5330 vab-12(dx25) III; him-8(e1489) IV. Vab XX hermaphrodites and XO males, viable at all temperatures. Adult hermaphrodite tail spike is invariably shortened and/or vacuolated, often also abnormal in larvae. Possible excretory cell abnormalities. Rays of adult male tail variably abnormal, other structures normal; males can mate.
CB5595 unc-85(e1414) II; him-8(e1489) IV. Unc hermaphrodites and males. Mate have variably abnormal tail anatomy. ME 0.
CB5600 ccIs4251 I; him-8(e1489) IV. ccIs4251 [(pSAK2) myo-3p::GFP::LacZ::NLS + (pSAK4) myo-3p::mitochondrial GFP + dpy-20(+)] I. Superficially WT hermaphrodites and males expressing GFP in nuclei and mitochondria of body wall muscles. Fluorescent body wall muscle nuclei can be seen by dissecting microscope with epifluoresence optics. Males mate poorly (ME 1/2). ccIs4251 mapped to LGI, + 2.5.
CB6503 bgIs312 I; him-8(e1489) IV; bus-17(e2923) X. bgIs312 [pes-6::GFP]. GFP expression in excretory cell only. Bus (resistant to infection by M. nematophilum and Leucobacter Verde2), hypersensitive to Leucobacter Verde1, bleach-sensitive, drug-sensitive; Him (high incidence of males). e2923 is a Mos1 insertion in bus-17 (ZK678.8). Reference: Yook & Hodgkin (2007), Genetics 175: 681-697
CB7151 him-8(e1489) IV; ptr-15(e2710) V. Him. Bus (resistant to M. nematophilum); hypersensitive to Leucobacter Verde1. References: Gravato-Nobre et al. (2005) PMID: 16079230. Hodgkin et al (in preparation).
CB7476 him-8(e1489) IV; galt-1(op497) V. Him. Resistant to fungal toxin CGL2; weakly resistant to Leucobacter Verde1. Him strain derived from pmk-1;galt-1 double mutant. References: Butschi et al (2010). O'Rourke et al (in preparation).
DA563 eat-9(e2337) I; him-8(e1489) IV. Abnormal feeding. Irregular pumping. Throws males.
DA631 eat-3(ad426) II; him-8(e1489) IV. Abnormal feeding. Slow, irregular feeding. eat-3 exhibits strong maternal effect; dauers seldom recover; Him.
DR284 unc-32(e189) III; him-8(e1489) IV. Unc-coiler. Segregates males. M-MATING-NO SUCCESS.
DR285 sqt-1(e1350) II; him-8(e1489) IV. Dpy. Heterozygotes Roll. Segregates males. M-MATING-NO SUCCESS.
DR286 him-8(e1489) IV; unc-58(e665) X. Semi-dominant Unc-shaker. Segregates males.
DR288 dpy-13(e184) him-8(e1489) IV. Dpy. Segregates males. Semi-dominant.
DR290 unc-54(m36) I; him-8(e1489) IV. Severe Unc. Segregates males. M-MATING-NO SUCCESS. Semi-dominant. Heterozygotes are slow moving.
DR292 che-3(e1379) I; him-8(e1489) IV. Dauer defective. Segregates males. Chemotaxis defective (Na+).
DS72 mat-3(ax68) III; him-8(e1489) IV. Temperature sensitive. Maintain at 15C.
DS73 mat-3(or180) III; him-8(e1489) IV. Temperature sensitive. Maintain at 15C.
DS77 mat-1(ax161) I; him-8(e1489) IV. Temperature sensitive. Maintain at 15C.
DS80 mat-1(ax144) I; him-8(e1489) IV. Temperature sensitive. Maintain at 15C.
DU23 spe-15(ok153) dpy-5(e61) I; him-8(e1489) IV; sDp2 (I;f). Animals with the duplication are WT. Animals which have lost the duplication are Dpy and Sterile. Throws males.
DY164 unc-32(e189) syIs80 III; him-8(e1489) IV. syIs80 [(pPGF11.13) lin-11::GFP + unc-119(+)] III. Animals are Unc. GFP fluorescence is observed in vulval cells, uterine pi cells and VC neurons.
DZ205 dsh-2(ez25)/mIn1 [mIs14 dpy-10(e128)] II; him-8(e1489) IV. mIs14 [myo-2p::GFP + pes-10p::GFP]. Him. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP+ heterozygotes, Dpy bright GFP+ (mIn1 homozygotes), and Egl non-GFP ez25 homozygotes. Pick WT dim GFP and check for correct segregation of progeny to maintain.
DZ224 him-8(e1489) IV; ezIs1 X. ezIs1[K09C8.2::GFP + rol-6(su1006)]. ezIs1 was integrated with pPD95.65(K09C8.2 promoter and unc-54 3') and pRF4. Worms are 100% Rollers. GFP is expressed in male seminal vesicle and vas deferens cells. No expression in the hermaphrodite gonad is observed.
DZ240 fkh-6(ez16)/mIn1 [dpy-10(e128) mIs14] II; him-8(e1489) IV. Heterozygotes are WT and GFP+ in the pharynx. mIn1[dpy-10(e128) mIs14] homozygotes are Dpy and GFP+ in the pharynx. Homozygous fkh-6(ez16) hermaphrodites are sterile and have gonadogenesis defects. Homozygous fkh-6(ez16) males are sterile and strong Gon, "white patch" phenotype. 25% of males have a hermaphrodite vulval structure.
DZ325 ezIs2 III; him-8(e1489) IV. ezIs2 [fkh-6::GFP + unc-119(+)]. ezIs2 was integrated with pPD95.69 (fkh-6 promoter and unc-54 3') and pMM106b (unc-119(+)). Worms are 100% non-Unc (the unc-119 background has been crossed out). GFP expression in adult hermaphrodite spermatheca is bright and weak staining is also observed in the proximal sheath cells. Weak GFP staining is also observed in Z1/Z4 cells in both sexes.
DZ390 ezIs10 II; unc-119(ed3) III; him-8(e1489) IV. ezIs10 [(pWY3) lin-32::GFP + unc-119(+)].
DZ393 mab-3(e1240) unc-4(e120) ref-1(ez11) II; him-8(e1489) IV. Unc (cannot back). WT male tail.
EE67 him-8(e1489) IV; mup-2(e2346) X. Temperature sensitive. At 15C the animals are essentially WT, but with a reduced brood; males mate well. Embryos raised at 25C will result in 3-fold/L1 arrest (Mup). Larval shift to 25C will result in sterile hermaphrodites but males that are fertile.
EG8040 oxTi302 I; oxTi75 II; oxTi411 unc-119(ed3) III; him-8(e1489) IV. oxTi302 [eft-3p::mCherry::tbb-2 3'UTR + Cbr-unc-119(+)]; inserted in Chr. I: 10,166,145. oxTi75 [eft-3p::GFP::H2B::tbb-2 3'UTR + unc-18(+)] II; inserted in Chr. II: 5,448,544. oxTi411 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)] inserted in Chr. III: 6,076,837. Him. Somewhat sick at higher temperatures. Maintain at 20C or below. This mapping strain uses three dominant fluorescent insertions that can be distinguished when scoring segregation. Please see www.wormbuilder.org for additional strain information.
EJ810 F25H2.5(ok314)/hT2 [dpy-18(h662)] I; +/hT2 [bli-4(e937)] III; him-8(e1489) IV. Him. Heterozygotes are WT and segregate WT, Sterile Evul and Dpys.
EU1073 him-8(e1489) IV; act-2(or295) V. Weakly semi-dominant, temperature sensitive, embryonic lethal mutant. 38% of embyros produced by homozygous mothers at 15C will hatch; 2% produced at 26C will hatch. Mutation is semi-dominant at 26C: 12% of embryos produced by heterozygous mothers hatched at 26C, and about 25% of the survivors were or295/or295, indicating the lethality is semi-dominant and maternal. Mutant embyros exhibit excess cortical actomyosin contractility (extra furrows and protrusions) in early embryonic cells, during interphase and most of mitosis. Throws males. or295 is a gga to aga substitution (G16R).
EU1077 mel-26(or543) I; him-8(e1489) IV. Temperature sensitive. Maintain at 15C. At 25C, hermaphrodites lay embryos that die. Transverse PO mitotic spindle, cytokinesis defects. Missense mutation at nucleotide #379 (cyt->tgt); amino acid #127 (R to C). Throws males.
EU1296 him-8(e1489) IV; act-2(or621) V. Strongly semi-dominant, temperature sensitive, embryonic lethal mutant. 40% of embyros produced by homozygous mothers at 15C will hatch; 0% produced at 26C will hatch. Mutation is semi-dominant at 26C: 85% of embyros produced by heterozygous mothers hatched at 26C. Mutant embryos exhibit excess coritical actomyosin contractility (extra furrows and protrusions) in early embryonic cells; during interphase and most of mitosis. Throws males. or621 is a tcc to gcc substitution (S15A).
EU1514 aph-1(or28)/hT2 I; +/hT2 [bli-4(e937) let-?(h661)] III; him-8(ec56) IV; lin-2(e1309) X. or28 is a non-conditional maternal-effect embryonic lethal mutant ; the anterior pharynx is absent. and embryonic lethality is 100% penetrant. Pick single WT heterozygous hermaphrodites to maintain strain; these hets fill up with a mix of dead embryos and larvae [11/16 dead embryos due to aneuploidy from translocation segregation and lethal homozygous hT2; vulvaless due to homozygous lin-2(e1309) mutation; about 10% of lin-2(e1309) worms are leaky and lay eggs/can be mated into]. The homozygous or28 hermaphrodites fill up with dead embryos. The lin-2 background helps to score embryonic lethality for both heterozygotes and or28 homozygotes. Strain produces a lot of males due to him-8(ec56). or28 is a G to D mis-sense mutation at amino acid position 123.
EU1515 aph-1(or28)/hT2 I; +/hT2 [bli-4(e937) let-?(h661)] III; him-8(ec56) IV. Him. or28 is a non-conditional maternal-effect embryonic lethal mutant ; the anterior pharynx is absent. and embryonic lethality is 100% penetrant. Pick single WT heterozygous hermaphrodites to maintain strain; these hets produce 11/16 dead embryos due to aneuploidy from translocation segregation and lethal homozygous hT2. The homozygous or28 hermaphrodites produce all dead embryos with defective pharyngeal development. or28 is a G to D mis-sense mutation at amino acid position 123.
EU407 mom-4(or39)/hT2 I; +/hT2 [bli-4(e937) let-?(h661)] III; him-8(e1489) IV; lin-2(e1309) X.
EU415 cyk-1(or36) unc-36(e251)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-8(e1489) IV. Heterozygotes are WT and segregate WT, Sterile Dpys and Uncs which give only dead eggs at all temperatures. Throws males.
EU447 unc-13(e1091) mom-4(or39)/hT2 I; +/hT2 [bli-4(e937) let-?(h661)] III; him-8(e1489) IV. Heterozygotes are WT. Segregates Uncs which give only embryonic lethals which lack gut and make excess mesoderm (E adopts an MS-like fate). or39 is a recessive maternal-effect embryonic lethal. Throws males.