| BC13816 |
dpy-5(e907) I; sEx13816. |
sEx13816 contains [rCes R107.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). |
| GS107 |
unc-32(e189) lin-12(n676n930) III; dpy-11(e224) sel-9(ar22) V. |
DpyUnc. At 25C ar22 recessively suppresses the Egl defect of n676n930. At 15C ar22 dominantly suppresses the 2 AC-Egl phenotype of n676n930. Suppresses the vulval lineage defects and proximal mitosis defects of lin-12. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. |
| GS60 |
unc-32(e189) lin-12(n676n930) III. |
Unc. Temperature sensitive hypomorphic lin-12 allele. At restrictive temperature of 25C: 100% Egl in unc-32 background (95% Egl without unc-32); 30% have 2 anchor cells, some proximal mitosis, abnormal vulval lineages but has opening; leaks some eggs; mates with males. At permissive temperature of 15C: 93% Egl(+) and 7% Egl(-) [lin-12(d)-like: no anchor cell and no vulval opening]. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. |
| GS776 |
unc-32(e189) lin-12(n676n930) III; unc-42(e270) sel-11(ar39) V. |
Unc. At 25C ar39 recessively suppresses the Egl phenotype of n676n930. At 15C a high percentage of hermaphrodites have a 0 AC-Egl phenotype. ar39 recessively suppresses vulval lineage defects and proximal mitosis. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. |
| GS807 |
unc-32(e189) lin-12(n676n930) III; sqt-3(sc8) sel-1(e1948) V. |
RollerUnc. At 25C e1948 recessively suppresses the Egl phenotype of n676n930. At 15C a high percentage of hermaphrodites have a 0 AC-Egl phenotype. e1948 recessively suppresses vulval lineage defects and proximal mitosis. sc8 previously called rol-4(sc8). See also WBPaper00002448. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. |
| GS878 |
unc-32(e189) lin-12(n676n930) III; lon-3(e2175) sel-10(ar41) V. |
Long. Unc. At 15C ar41 suppresses the 2 AC (anchor cell) phenotype of n676n930. (Strain GS878 does not contain sel(arX) and therefore does not suppress the Egl phenotype at 25C.) See also WBPaper00002966. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. |
| GS883 |
dpy-5(e61) sel(ar40) I; unc-32(e189) lin-12(n676n930) III. |
DpyUnc. ar40 is a semi-dominant suppressor. At 25C ar40 suppresses the Egl phenotype of ne676n930. At 15C a high percentage of hermaphrodites have a 0 AC-Egl phenotype. ar40 suppresses proximal mitosis. ar40 does not suppress vulval lineage defects. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. |
| JK1195 |
lin-12(q269) glp-1(q231)/unc-32(e189) III. |
Heterozygotes are WT and segregate WT, Uncs, and Lags (ts). Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. |
| JK1313 |
lin-12(n941) glp-1(q46)/dpy-19(e1259) unc-69(e587) III. |
Heterozygotes are WT and segregate WT, Dpy(ts) Uncs, and Lag L1 lethals (not ts). Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. |
| JT5205 |
lin-12(n950) III; lag-2(sa37) V. |
lag-2(sa37) is a semi-dominant suppressor of lin-12(n302sd). |
| JT5210 |
lin-12(n302) III; emb-4(sa44) V. |
emb-4(sa44) is a recessive suppressor of lin-12(n302sd). |
| JT5218 |
sup-17(sa38) I; lin-12(n302) III. |
sa38 is a weak Egl and a moderate suppressor of lin-12(gf). |
| JT5222 |
sup-19(sa45) I; lin-12(n302) III. |
sa45 is a weak Egl and a moderate suppressor of lin-12(gf). |
| JT5277 |
sel-8(sa54) lin-12(n302) III. |
sel-8(sa54) is a recessive suppressor of lin-12(n302sd). |
| JT5283 |
lin-12(n302) III; sel-7(n1253) X. |
sel-7(n1253) is a recessive suppressor of lin-12(n302sd). |
| MJ61 |
emb-5(hc61) lin-12(ar170) III. |
ts embryonic lethal. Grows at 15C, 20C. Lethal at 25C. [Also contains a temperature sensitive lin-12 hypomorphic allele called ar170. Has 2 anchor cells. Jane Hubbard, 3/96 See WBPaper00002483. See GS1369 for emb-5 reference strain.] |
| MT1035 |
lin-12(n137n460) III. |
WT at 25C. Muv and Egl at 15C. |
| MT1329 |
lin-12(n302) III; him-5(e1467) V. |
Vulvaless. Segregates males which mate well. |
| MT1337 |
lin-12(n137) III; nT1 (IV;V). |
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| MT1341 |
lin-12(n302) III; nT1 (IV;V). |
Vulvaless. |
| MT1965 |
lin-12(n941)/eT1 III; him-5(e1467)/eT1 [him-5(e1467)] V. |
Heterozygotes are WT and segregate WT, Muv or Steriles (homozygous n941), Unc-36 (homozygous eT1), and dead eggs. Pick wild-type and check for correct segregation of progeny to maintain. n941 is a lin-12 null allele. lin-12(n941) homozygotes are Muv or Ste. e1467 is also carried on eT1. |
| MT2021 |
lin-12(n952) III; him-5(e1467) V. |
Muv. Throws males. |
| MT2343 |
dpy-19(e1259) lin-12(n137)/unc-32(e189) lin-12(n137n720) III. |
Heterozygotes are Muv and Egl (n137 is semi-dominant) and segregate DpyMuv and Unc lethals (most arrest as L1-L3, the few survivors are sterile, scrawny, Unc and have a large ventral blip at hte position of the vulva). Maintain by picking non-Dpy non-Unc. |
| MT2351 |
lin-10(e1439) I; dpy-19(e1259) lin-12(n137)/unc-32(e189) lin-12(n137n720) III. |
Heterozygotes are Muv. Segregates DpyMuv and Unc. |
| MT2982 |
sel-4(n1259) II; lin-12(n302) III. |
sel-4(n1259) is a recessive suppressor of lin-12(n302sd). |
| MT3000 |
sel-5(n1254) lin-12(n302) III. |
sel-5(n1254) is a recessive suppressor of lin-12(n302sd). n1254 is cold sensitive. |
| MT302 |
lin-12(n302) III. |
Vulvaless. Semi-dominant. Males mate. |
| MT3063 |
lin-12(n950) III. |
Muv. Semi-dominant. |
| MT688 |
lin-12(n137)/unc-32(e189) III; him-5(e1467) V. |
Heterozygotes are Muv and segregate Muv, Unc and males. Maintain by picking Muv which throw Uncs. |
| NOA1 |
lin-12(n137n460) III; him-5(e1467) V; let-7(n2853) X. |
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| NOA2 |
lin-12(n137n460) III; him-5(e1467) V; unc-3(e151) let-7(mn112) X. |
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| NOA3 |
lin-41(ma104) I; lin-12(n137n460) III. |
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| NOA4 |
sqt-1(sc13) lin-29(n333) II; lin-12(n137n460) III. |
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| VC1955 |
lin-12(ok2215) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). |
R107.8. Homozygous sterile deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ok2215 homozygotes (sterile with vulval blip). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: AATCTTTTCTCGCAGCTCCA. External right primer: CATACATTTGCGTGTGTCCC. Internal left primer: GGGCTGTCATTCCGTTTCTA. Internal right primer: AAACCTGGGAACACATCGAC. Internal WT amplicon: 3327 bp. Deletion size: 1227 bp. Deletion left flank: ATTAATTCTGTTGGTGTGGTTTGGTTTTAT. Deletion right flank: GATTTCTAGAAAACAAACTGGTTGCTTGAA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807 |
| VT757 |
lin-28(n719) I; lin-12(n137n460) III. |
Only gives a decent brood size at 20C. At 15C: Muv/Blip. At 20C: some Muv/Blip, some Blip but not Muv. At 25C: Not Muv, but do have Blip (due to lin-12). Egl- at all temps. |