Gene Information: lin-12

Namelin-12 View on WormBase
Species C. elegans
SequenceR107.8
Genetic positionIII:0.14 +/- 0.003 cM
Genomic positionIII: 9060221..9071472

Strains carrying this gene

Strain Genotype Description
BC13816 dpy-5(e907) I; sEx13816. sEx13816 contains [rCes R107.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525).
GS107 unc-32(e189) lin-12(n676n930) III; dpy-11(e224) sel-9(ar22) V. DpyUnc. At 25C ar22 recessively suppresses the Egl defect of n676n930. At 15C ar22 dominantly suppresses the 2 AC-Egl phenotype of n676n930. Suppresses the vulval lineage defects and proximal mitosis defects of lin-12. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
GS60 unc-32(e189) lin-12(n676n930) III. Unc. Temperature sensitive hypomorphic lin-12 allele. At restrictive temperature of 25C: 100% Egl in unc-32 background (95% Egl without unc-32); 30% have 2 anchor cells, some proximal mitosis, abnormal vulval lineages but has opening; leaks some eggs; mates with males. At permissive temperature of 15C: 93% Egl(+) and 7% Egl(-) [lin-12(d)-like: no anchor cell and no vulval opening]. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
GS776 unc-32(e189) lin-12(n676n930) III; unc-42(e270) sel-11(ar39) V. Unc. At 25C ar39 recessively suppresses the Egl phenotype of n676n930. At 15C a high percentage of hermaphrodites have a 0 AC-Egl phenotype. ar39 recessively suppresses vulval lineage defects and proximal mitosis. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
GS807 unc-32(e189) lin-12(n676n930) III; sqt-3(sc8) sel-1(e1948) V. RollerUnc. At 25C e1948 recessively suppresses the Egl phenotype of n676n930. At 15C a high percentage of hermaphrodites have a 0 AC-Egl phenotype. e1948 recessively suppresses vulval lineage defects and proximal mitosis. sc8 previously called rol-4(sc8). See also WBPaper00002448. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
GS878 unc-32(e189) lin-12(n676n930) III; lon-3(e2175) sel-10(ar41) V. Long. Unc. At 15C ar41 suppresses the 2 AC (anchor cell) phenotype of n676n930. (Strain GS878 does not contain sel(arX) and therefore does not suppress the Egl phenotype at 25C.) See also WBPaper00002966. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
GS883 dpy-5(e61) sel(ar40) I; unc-32(e189) lin-12(n676n930) III. DpyUnc. ar40 is a semi-dominant suppressor. At 25C ar40 suppresses the Egl phenotype of ne676n930. At 15C a high percentage of hermaphrodites have a 0 AC-Egl phenotype. ar40 suppresses proximal mitosis. ar40 does not suppress vulval lineage defects. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
JK1195 lin-12(q269) glp-1(q231)/unc-32(e189) III. Heterozygotes are WT and segregate WT, Uncs, and Lags (ts). Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
JK1313 lin-12(n941) glp-1(q46)/dpy-19(e1259) unc-69(e587) III. Heterozygotes are WT and segregate WT, Dpy(ts) Uncs, and Lag L1 lethals (not ts). Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
JT5205 lin-12(n950) III; lag-2(sa37) V. lag-2(sa37) is a semi-dominant suppressor of lin-12(n302sd).
JT5210 lin-12(n302) III; emb-4(sa44) V. emb-4(sa44) is a recessive suppressor of lin-12(n302sd).
JT5218 sup-17(sa38) I; lin-12(n302) III. sa38 is a weak Egl and a moderate suppressor of lin-12(gf).
JT5222 sup-19(sa45) I; lin-12(n302) III. sa45 is a weak Egl and a moderate suppressor of lin-12(gf).
JT5277 sel-8(sa54) lin-12(n302) III. sel-8(sa54) is a recessive suppressor of lin-12(n302sd).
JT5283 lin-12(n302) III; sel-7(n1253) X. sel-7(n1253) is a recessive suppressor of lin-12(n302sd).
MJ61 emb-5(hc61) lin-12(ar170) III. ts embryonic lethal. Grows at 15C, 20C. Lethal at 25C. [Also contains a temperature sensitive lin-12 hypomorphic allele called ar170. Has 2 anchor cells. Jane Hubbard, 3/96 See WBPaper00002483. See GS1369 for emb-5 reference strain.]
MT1035 lin-12(n137n460) III. WT at 25C. Muv and Egl at 15C.
MT1329 lin-12(n302) III; him-5(e1467) V. Vulvaless. Segregates males which mate well.
MT1337 lin-12(n137) III; nT1 (IV;V).
MT1341 lin-12(n302) III; nT1 (IV;V). Vulvaless.
MT1965 lin-12(n941)/eT1 III; him-5(e1467)/eT1 [him-5(e1467)] V. Heterozygotes are WT and segregate WT, Muv or Steriles (homozygous n941), Unc-36 (homozygous eT1), and dead eggs. Pick wild-type and check for correct segregation of progeny to maintain. n941 is a lin-12 null allele. lin-12(n941) homozygotes are Muv or Ste. e1467 is also carried on eT1.
MT2021 lin-12(n952) III; him-5(e1467) V. Muv. Throws males.
MT2343 dpy-19(e1259) lin-12(n137)/unc-32(e189) lin-12(n137n720) III. Heterozygotes are Muv and Egl (n137 is semi-dominant) and segregate DpyMuv and Unc lethals (most arrest as L1-L3, the few survivors are sterile, scrawny, Unc and have a large ventral blip at hte position of the vulva). Maintain by picking non-Dpy non-Unc.
MT2351 lin-10(e1439) I; dpy-19(e1259) lin-12(n137)/unc-32(e189) lin-12(n137n720) III. Heterozygotes are Muv. Segregates DpyMuv and Unc.
MT2982 sel-4(n1259) II; lin-12(n302) III. sel-4(n1259) is a recessive suppressor of lin-12(n302sd).
MT3000 sel-5(n1254) lin-12(n302) III. sel-5(n1254) is a recessive suppressor of lin-12(n302sd). n1254 is cold sensitive.
MT302 lin-12(n302) III. Vulvaless. Semi-dominant. Males mate.
MT3063 lin-12(n950) III. Muv. Semi-dominant.
MT688 lin-12(n137)/unc-32(e189) III; him-5(e1467) V. Heterozygotes are Muv and segregate Muv, Unc and males. Maintain by picking Muv which throw Uncs.
NOA1 lin-12(n137n460) III; him-5(e1467) V; let-7(n2853) X.
NOA2 lin-12(n137n460) III; him-5(e1467) V; unc-3(e151) let-7(mn112) X.
NOA3 lin-41(ma104) I; lin-12(n137n460) III.
NOA4 sqt-1(sc13) lin-29(n333) II; lin-12(n137n460) III.
PS3996 lin-10(e1439) I; dpy-19(e1259) lin-12(n137)/unc-32(e189) lin-12(n137n720) III; syIs57. syIs57 [chd-3::CFP]. Anchor cell present in approx. 1% d/o animals.
VC1955 lin-12(ok2215) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). R107.8. Homozygous sterile deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ok2215 homozygotes (sterile with vulval blip). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: AATCTTTTCTCGCAGCTCCA. External right primer: CATACATTTGCGTGTGTCCC. Internal left primer: GGGCTGTCATTCCGTTTCTA. Internal right primer: AAACCTGGGAACACATCGAC. Internal WT amplicon: 3327 bp. Deletion size: 1227 bp. Deletion left flank: ATTAATTCTGTTGGTGTGGTTTGGTTTTAT. Deletion right flank: GATTTCTAGAAAACAAACTGGTTGCTTGAA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VT757 lin-28(n719) I; lin-12(n137n460) III. Only gives a decent brood size at 20C. At 15C: Muv/Blip. At 20C: some Muv/Blip, some Blip but not Muv. At 25C: Not Muv, but do have Blip (due to lin-12). Egl- at all temps.