||dpy-5(e907) I; sEx13756.
||sEx13756 [rCesZK370.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525).
||Small. Recessive. Male spicules abnormal. M-MATING-NO SUCCESS. Synthetic lethal common.
||sma-2(e502) emb-30(tn377) ced-7(n1892) unc-69(e587) III.
||Temperature sensitive emb-30 allele. Maintain at 15C. Small. Unc. Cell death abnormal.
||sma-2(e502) tnDf2/sma-2(e502) ced-7(n1892) unc-69(e587) III.
||Heterozygotes are SmaCed and segregate SmaCed, SmaCedUnc and dead eggs. Maintain by picking SmaCed. tnDf2 is not transmitted well by males (i.e. tnDf2/+ males have a low mating efficiency).
||unc-32(e189) tnDf2/sma-2(e502) ced-7(n1892) unc-69(e587) III.
||Heterozygotes are Ced and segregate Ced, dead eggs and SmaCedUncs. Maintain by picking Ced. tnDf2 is not transmitted well by males (i.e. tnDf2/+ males have a low mating efficiency).
||sma-2(e502) III; sma-5(n678) X.
||Very small. Small brood size (about 1/10 that of WT). Grows very slowly. Reference: Watanabe, N et al. Genes to Cells 2007. 12(5):603-609.
||nDf20/sma-2(e502) unc-32(e189) III.
||Heterozygotes are WT and segregate WT, SmaUnc and dead eggs. Maintain by picking WT.
||sma-2(e502) ced-7(n1892) III.
||Small. Maternal effect persistent cell corpses.
||sma-2(e502) ced-7(n1892) unc-69(e587) III.
||sma-2(e502) III; ccIs55 V.
||ccIs55 [unc-54::lacZ + sup-7(st5)] V. Small body size. Slow growth. Strong lacZ staining in body wall.
||sma-2(ok3109)/qC1 [dpy-19(e1259) glp-1(q339)] III.
||ZK370.2. Apparent homozygous lethal deletion chromosome balanced by glp-1- and dpy-19-marked recombination suppressor. Heterozygotes are WT, and segregate WT, sterile ts-Dpy qC1 homozygotes, and ok3109 homozygotes (arrest stage/phenotype undetermined). Pick WT and check for correct segregation of progeny to maintain. External left primer: GTCGCTGATTCCAGTCGTTT. External right primer: AGCTAAATCCGCACACGAAC. Internal left primer: TAAACAGCATGCGGTGGAAT. Internal right primer: TGAAAAATTTGGCTCCGAGT. Internal WT amplicon: 1222 bp. Deletion size: 707 bp. Deletion left flank: AATAACTTTGAGAGGGAAAAGGTTACGAAA. Deletion right flank: TCACTGAAGATTTTCGATATGGAGATTTTT. Insertion sequence: TCACTGAAGATTTTCGATATGTATTTGAGAGGGAAAAGGTTACGAAA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
||sma-2(e502) ced-7(n1892) cdk-1(ne236)/qC1 [dpy-19(e1259) glp-1(q339) III.
||Heterozygotes are WT and segregate WT, Smalls which produces dead eggs, and Dpy Steriles. Throws males.