Gene Information: dyf-3

Namedyf-3 View on WormBase
Species C. elegans
Genetic positionIV:-4.54 +/- 0.026 cM
Genomic positionIV: 3396581..3399231

Strains carrying this gene

Strain Genotype Description
BC13318 dpy-5(e907) I; sEx13318. sEx13318 [rCesC04C3.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525)..
SP1603 dyf-3(m185) IV. Defective in dye filling (FITC or DiO) of amphid and phasmid neurons. Chemotaxis defective.
VC1631 dyf-3(gk760) IV/nT1 [qIs51] (IV;V). C04C3.5. Homozygous sterile deletion chromosome balanced by GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP gk760 homozygotes (sterile adult). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: ACCACCCATCATGTTACCGT. External right primer: GAAGCTCGTCGACCGTAGTC. Internal left primer: TCACGCATCCTTCTTCTCCT. Internal right primer: TTGCAGGGAGTTTCTATGGG. Internal WT amplicon: 1853 bp. Deletion size: 736 bp. Deletion left flank: TACTCGTCCATATACTGAGGTCGGAAGGAC. Deletion right flank: CGGACCTCCTGCATCTGAACTTTCGACAGT. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
WX1257 dyf-3(og22) IV; ogEx49. ogEx49 [dyf-3(+) + rol-6(su1006)]. Maintain by picking Rollers. Animals which have lost the array are Dyf (DiI).
WX737 dyf-3(og22) IV. Dyf (DiI), chemotaxis defective towards IAA. Preliminary results show increased longevity.