||dpy-5(e907) I; sEx12660.
||sEx12660 [rCes R13A5.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525).
||Slow growth, Slow movement. ogr1 is a 1.2 kb deletion causing a frameshift at residue 54 and premature stop at residue 83. Reference: Ogurusu T, et al. Biochem Biophys Res Commun. 2015 Aug 7;463(4):994-8. PMID: 26079877
||R13A5.9. External left primer: TTCACTTCCACCACCACCTT. External right primer: TTTCTGGACCATTTTGGAGC. Internal left primer: ATTCTCGCCCTTGCTTTCTC. Internal right primer: AATTTGCCAGTCGTTTCAGG. Internal WT amplicon: 1266 bp. Deletion size: 518 bp. Deletion left flank: GGATGATTTCGGAATGTATCCGAATAATCG. Deletion right flank: CGCTGAAATTAAAATAATTTATTTTGAATC. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807