| BC11068 |
dpy-5(e907) I; sEx11068. |
sEx11068 [rCesF46A9.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). |
| CB398 |
mec-8(e398) I. |
Slightly Dpy. Sluggish. Recessive. Mechanosensory abnormal. M-MATING++ 1-10%WT. |
| JK1534 |
ces-1(n703) qDf5/unc-29(e193) mec-8(e398) blmp-1(s71) I. |
Heterozygotes are Ces and grow slowly. Hets segregate DpyUncs and dead eggs. ces-1(n703) is dominant. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. |
| JK1561 |
ces-1(n703) qDf15/unc-29(e193) mec-8(e398) lin-11(n566) I. |
Heterozygotes are Ces and segregate VulUncs and dead eggs. ces-1(n703) is dominant. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. |
| SP1560 |
mec-8(u218) I. |
Temperature sensitive mec-8 allele. |
| SP1564 |
mec-8(u218) smu-1(mn609) I; unc-52(e669su250ts) II. |
Temperature sensitive mec-8 allele, mechanosensory abnormal at 25 C. Reference: Spike CA, et al. Mol Cell Biol. 2001 Aug;21(15):4985-95. |
| SP1750 |
mec-8(u74) I; mnEx2. |
mnEx2 [mec-8(+) + rol-6(su1006)]. Rollers. Pick rollers to maintain. |
| SP2123 |
mec-8(u218) smu-1(mn602) I; unc-52(e669su250ts) II. |
Temperature sensitive mec-8 allele, mechanosensory abnormal at 25 C. Reference: Spike CA, et al. Mol Cell Biol. 2001 Aug;21(15):4985-95. |
| TU151 |
mec-8(u303) I. |
Mechanosensory abnormal. |
| TU166 |
mec-8(u314) I. |
Reference: Lundquist and Herman 1994 Genetics 138: 83. |
| TU218 |
mec-8(u218) I. |
Temperature sensitive. Mechanosensory abnormal at 25 C. Maintain at 15 C. Reference: Chalfie & Sulston (1981) Dev Biol 82:358. |
| TU3135 |
mec-8(u218) I; rde-1(ne219) V; uIs46. |
uIs46 [rde-1p::mec-2(intron9)::rde-1) + ceh-22::GFP]. Temperature sensitive; maintain at 15 C. RNAi-sensitive at 15 C. Mechanosensory abnormal at 25 C. Uses the ninth intron of mec-2, whose splicing depends on mec-8, to produce temperature-sensitive expression and temperature-sensitive RNAi. Reference: Calixto et al. (2010) Nature Methods 7:407-11. |
| VC1594 |
mec-8(ok2043) I. |
F46A9.6. Superficially wild type. External left primer: ATAGCAAGTGTGTGAGGGGG. External right primer: CCGACACAACATTCGACATC. Internal left primer: CCTCGATCGTTGGCTGTTAT. Internal right primer: ATTTCTTTGTCGCCCCTTTT. Internal WT amplicon: 2265 bp. Deletion size: 1117 bp. Deletion left flank: TCGTTGGCTGTTATCTGGAATCCTTGTAGT. Deletion right flank: TGTTGCTCATTGAAAAGAGCTGCTGATTGA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807 |