||aak-1(tm1944) III; aak-2(ok524) X; uthEx202.
||uthEx202 [crtc-1p::crtc-1 cDNA::tdTomato::unc-54 3'UTR + rol-6(su1006)]. Pick Rollers to maintain. Reference: Mair W, et al. Nature. 2011 Feb 17;470(7334):404-8.
||dpy-5(e907) I; sEx10615.
||sEx10615 contains [rCesT01C8.1a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525).
||aak-2(ok524) X; adIs2122.
||adIs2122 [lgg-1::GFP + rol-6(su1006)]. Rollers. Reference: Egan DF, et al. Science. 2011 Jan 28;331(6016):456-61.
||sir-2.1(ok434) IV; aak-2(ok524) X.
||Slow growing. Maintain under normal conditions. Derived from parental strains RB754 [aak-2(ok524)] and VC199 [sir-2.1(ok434)]. Reference: Schmeisser S, et al. Molecular Metabolism February 15, 2013. DOI: 10.1016/j.molmet.2013.02.002.
||aak-1(tm1944) III; aak-2(gt33) X.
||Hypersensitive to oxidative stress; more sensitive to the stress than either of the cognate single mutants. Parental aak-1(tm1944) strain outcrossed 8 times; parental aak-2(gt33) strain outcrossed 3 times. Reference: Lee H, et al. J Biol Chem. 2008 May 30;283(22):14988-93.
||feIs4 V; aak-2(ok524) X.
||feIs4 [sur-5p::luciferase::GFP + rol-6(su1006)] V. Rollers. Strain is bioluminescent when provided with exogenous D-luciferin (potassium salt) due to sur-5 promoter driving expression of firefly (Photinus pyralis) luciferase (lacking the peroxisome tagging signal) fused in-frame to GFP(S65C). Pick animals with high levels of fluorescence to retain expression of luciferase transgene. This strain is for academic use only. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. References: Lagido C, et al. BMC Physiol. 2008 Apr 2;8:7. McLaggan D, et al. PLoS One. 2012;7(10):e46503. Lagido C, et al. Toxicol Sci. 2009 May;109(1):88-95.
||T01C8.1. Homozygous. Outer Left Sequence: TCATTTGCTGCAACTTCCTG. Outer Right Sequence: ATACGTGGCATTTACGGAGG. Inner Left Sequence: ATGTCGTTGGAAAGATTCGC. Inner Right Sequence: AAGGAGTGCTTAACGAGCCA. Inner primer WT PCR product: 2741. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
||Hypersensitive to oxidative stress, heat, and UV. 606 bp deletion from nucleotide 22 of Exon 3 to nucleotide 160 of Intron 3.