Gene Information: let-765

Namelet-765 View on WormBase
Species C. elegans
Genetic positionIII:-1.06 +/- 0.003 cM
Genomic positionIII: 6592525..6601173

Strains carrying this gene

Strain Genotype Description
BC4270 dpy-17(e164) let-765(s2630) unc-32(e189) III; sDp3 (III;f). Maintain by picking Uncs. DpyUncs arrest at mid-larval stage. s2630 pka let-745.
VC1622 let-765(ok2058) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). F20H11.2. Homozygous lethal deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ok2058 homozygotes (early- to mid-larval arrest). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: CAATCGTATTGCTGCTTCCA. External right primer: CGGAGCTGGTGTAGGAAAAG. Internal left primer: GTCCATTCGAATCTTTCCGA. Internal right primer: TGCTGAACGTGATCTTCGAG. Internal WT amplicon: 3229 bp. Deletion size: 1540 bp. Deletion left flank: CACAGATTCTAGATGAAGTGATAAAATCCG. Deletion right flank: TTTTGAAGTTCTAAGACCATTCGTCCAGTT. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807