| BC4860 |
dpy-17(e164) let-743(s2830) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). |
Maintain by picking Uncs. |
| BC4862 |
let-736(s2832) dpy-17(e164) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). |
Maintain by picking Uncs. |
| BC4863 |
dpy-17(e164) cyk-1(s2833) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). |
Maintain by picking Uncs. cyk-1 was previously known as let-794 (in Stewart et al 1998). |
| BC4864 |
dpy-17(e164) let-837(s2834) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). |
Maintain by picking Uncs. |
| BC4865 |
dpy-17(e164) let-818(s2835) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). |
Maintain by picking Uncs. |
| BC4866 |
dpy-17(e164) let-810(s2836) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). |
Maintain by picking Uncs. |
| BC4867 |
let-734(s2837) dpy-17(e164) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). |
Maintain by picking Uncs. pka let-833(s2837). |
| BC4868 |
let-791(s2838) dpy-17(e164) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). |
Maintain by picking Uncs. |
| BC4869 |
dpy-17(e164) let-838(s2839) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). |
Maintain by picking Uncs. |
| BC4870 |
dpy-17(e164) let-775(s2840) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). |
Maintain by picking Uncs. |
| BC4873 |
let-819(s2843) dpy-17(e164) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). |
Maintain by picking Uncs. |
| BC4874 |
dpy-17(e164) let-809(s2844) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). |
Maintain by picking Uncs. |
| BC4875 |
let-820(s2845) dpy-17(e164) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). |
Maintain by picking Uncs. |
| BC4876 |
let-840(s2846) dpy-17(e164) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). |
Maintain by picking Uncs. |
| BC4878 |
let-822(s2848) dpy-17(e164) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). |
Maintain by picking Uncs. |
| BC4879 |
dpy-17(e164) let-795(s2849) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). |
Maintain by picking Uncs. |
| BC4880 |
dpy-17(e164) let-798(s2850) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). |
Maintain by picking Uncs. DpyUncs die as embryos. |
| BC4881 |
dpy-17(e164) let-842(s2851) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). |
Maintain by picking Uncs. |
| BC4882 |
dpy-17(e164) let-799(s2852) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). |
Maintain by picking Uncs. |
| BC4883 |
let-831(s2853) dpy-17(e164) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). |
Maintain by picking Uncs. |
| BC4997 |
dpy-17(e164) let-755(s2861) unc-32(e189) III; sDp3 (III;f). |
Maintain by picking Uncs. DpyUncs arrest at early-mid larval stages. |
| BC5078 |
dpy-17(e164) mel-32(s2518) unc-32(e189) III; sDp3 (III;f). |
Maintain by picking Unc-32 animals. Dpy animals that have lost sDp3 should also be Mel; expected Mel phenotype is embryonic lethal. |
| BW1434 |
dpy-17(e164) III; him-8(e1489) IV; her-1(y101hv1) unc-42(e270) V; ctDp11 (III;V;f). |
Wild type hermaphrodites and males which produce WT, Dpy, Unc and DpyUnc progeny due to mating or loss of the duplication. Pick WT hermaphrodites and males to maintain. |
| BW2041 |
pal-1(ct224) dpy-17(e164) ncl-1(e1865) unc-36(e251) III; svDp1 (III;f). |
svDp1 [sur-5::GFP] (III;f). svDp1 was derived by insertion of a sur-5::GFP marker into sDp3(III;f). Pick GFP+ wild-type to maintain. svDp1 rescues ct224. ct224 homozygotes (lacking GFP expression) show Nob phenotype: approximately 80% of homozygous embryos arrest at about the time of hatching with fairly normal anterior development but a severely deformed posterior with a variable knob-like shape; approximately 20% fail to enclose and do not hatch. The duplication is lost in about 30-40% of embryos. Reference: Edgar LG, et al. Dev Biol. 2001 Jan 1;229(1):71-88. |
| CB1295 |
dpy-17(e1295) III. |
Medium Dpy. |
| CB1345 |
dpy-17(e1345) III. |
Dpy. Temperature sensitive. |
| CB164 |
dpy-17(e164) III. |
Dpy. Larvae abnormal. M-MATING+++ 10-30%WT. |
| CB6233 |
dpy-17(e2898) dpy-31(e2770) III. |
Viable dumpy. Suppressor allele of dpy-17: lethality of dpy-31(e2770) suppressed by e2898. Reference: Novelli et al. (2006) PMID: 16452136. |
| CB6234 |
dpy-17(e2899) dpy-31(e2770) III. |
Weak dumpy. Lethal dpy-31 mutation suppressed by dpy-17 mutation. Reference: Novelli et al. (2006) PMID: 16452136. |
| CB7272 |
ccIs4251 I; mIs12 II; dpy-17(e164) III; frIs7 IV; uIs69 V. |
ccIs4251 [(pSAK2) myo-3p::GFP::LacZ::NLS + (pSAK4) myo-3p::mitochondrial GFP + dpy-20(+)] I. mIs12 [myo-2p::GFP + pes-10p::GFP + F22B7.9p::GFP] II. frIs7 [nlp-29p::GFP + col-12p::DsRed] IV. uIs69 [pCFJ90(myo-2p::mCherry) + unc-119p::sid-1] V. Mapping strain. This strain is homozygous for integrated fluorescence markers on LG I, II, IV and V, all of which are easily and independently scored using a fluorescent dissecting microscope, plus an easily scored visible marker (dpy-17) for LGIII. The good markers on all five autosomes facilitate linkage assignment of unmapped mutations, and enable rapid replacement of chromosomes when outcrossing heavily mutagenized strains such as those from the Million Mutation Project. |
| CGC17 |
unc-4(e120)/mT1 [umnIs6] II; dpy-17(e164)/mT1 [dpy-10(e128)] III. |
umnIs6 [eft-3p::NLS::tdTomato + HygroR, III:~5753000 (intergenic)] II. Heterozygotes are WT with dim red fluorescence, and segregate WT with dim red fluorescence, arrested mT1 aneuploids, sterile Dpys (mT1 homozygotes with more intense red fluorescence), and DpyUnc with no red fluorescence. Pick WT with dim red fluorescence and check for correct segregation of progeny to maintain. |
| CGC45 |
unc-4(e120)/mT1 [umnIs34] II; mT1 [dpy-10(e128)]/dpy-17(e164) III. |
umnIs34 [myo-2p::GFP + NeoR, III: 8856215 (intergenic)] II. Heterozygotes are wild-type GFP+, and segregate wild-type GFP+, DpyUnc non-GFP, sterile Dpy GFP+ mT1 homozygotes, and large numbers of arrested aneuploid embryos. Maintain by picking wild-type GFP+ and check for correct segregation of progeny to maintain. Derived by insertion of myo-2p::GFP transgene into mT1 balancer in parental strain DR1832 using CRISPR/Cas9. |
| CGC65 |
mT1/unc-4(e120) II; mT1 [dpy-10(e128) umnIs51]/dpy-17(e164) III. |
umnIs51 [myo-2p::mKate2 + NeoR, II: 11755713 (intergenic)] III. Heterozygotes are wild-type mKate2+, and segregate wild-type mKate2+, DpyUnc non-mKate2, sterile Dpy mKate2+ mT1 homozygotes, and large numbers of arrested aneuploid embryos. Maintain by picking wild-type mKate2+ and check for correct segregation of progeny to maintain. Derived by insertion of myo-2p::mKate2 transgene into mT1 balancer in parental strain DR1832 using CRISPR/Cas9. |
| CGC66 |
unc-4(e120)/mT1 [umnIs52] II; mT1 [dpy-10(e128)]/dpy-17(e164) III. |
umnIs52 [myo-2p::mKate2 + NeoR, III: 8856215 (intergenic)] II. Heterozygotes are wild-type mKate2+, and segregate wild-type mKate2+, DpyUnc non-mKate2, sterile Dpy mKate2+ mT1 homozygotes, and large numbers of arrested aneuploid embryos. Maintain by picking wild-type mKate2+ and check for correct segregation of progeny to maintain. Derived by insertion of myo-2p::mKate2 transgene into mT1 balancer in parental strain DR1832 using CRISPR/Cas9. |
| CGC68 |
mT1/unc-4(e120) II; mT1 [dpy-10(e128) umnIs54]/dpy-17(e164) III. |
umnIs54 [myo-2p::GFP + NeoR, II: 11755713 (intergenic)] III. Heterozygotes are wild-type GFP+, and segregate wild-type GFP+, DpyUnc non-mGFP, sterile Dpy GFP+ mT1 homozygotes, and large numbers of arrested aneuploid embryos. Maintain by picking wild-type GFP+ and check for correct segregation of progeny to maintain. Derived by insertion of myo-2p::GFP transgene into mT1 balancer in parental strain DR1832 using CRISPR/Cas9. |
| CX2914 |
nDf16/dpy-17(e164) unc-32(e189) III. |
Heterozygotes are WT and segregate WT, DpyUncs and dead eggs. The DpyUncs will outgrow the hets. |
| DR1832 |
mT1/unc-4(e120) II; mT1 [dpy-10(e128)]/dpy-17(e164) III. |
WT phenotype. Segregates WT, sterile Dpy mT1 homozygotes, Unc-4;Dpy-17 and large numbers of arrested aneuploid embryos. Pick WT and check for correct segregation of progeny to maintain. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to Don Riddle. |
| DV2275 |
daf-2(e1370) dpy-17(e164) III. |
|
| EA90 |
pag-1(ls2) dpy-17(e164) III. |
Dpy. pag-1(ls2) is recessive and has not visible phenotype by itself. It increases the expression of certain lacZ fusion genes such as lacZmec-7, unc-86lacZ and unc-4unc-76lacZ. |
| FF275 |
dpy-17(e164) unc-32(f123) ncl-1(e1865)/qC1 [dpy-19(e1259) glp-1(q339)] III. |
Heterozygotes are WT and segregate WT, Dpy Steriles (qC1 homozygotes), and embryonic lethals (f123 homozygotes) |
| FF276 |
dpy-17(e164) unc-32(f121) ncl-1(e1865)/qC1 [dpy-19(e1259) glp-1(q339)] III. |
Heterozygotes are WT and segregate WT, Dpy Steriles (qC1 homozygotes), and larval lethals (f121 homozygotes). g to a transition in exon 6 (2874), changing a Gly in Glu in ZK637.8 gene encoding a V-ATPase alpha subunit |
| GS311 |
unc-36(e251) evl-14(ar97)/dpy-17(e164) unc-32(e189) III. |
Heterozygotes are WT and segregate WT, DpyUncs and Sterile Unc-36 which have an everted vulva. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. |
| GS316 |
unc-36(e251) evl-14(ar96)/dpy-17(e164) unc-32(e189) III. |
Heterozygotes are WT and segregate WT, DpyUncs and Sterile Unc-36 which have an everted vulva. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. |
| GS320 |
unc-36(e251) evl-8(ar102)/dpy-17(e164) unc-32(e189) III. |
Heterozygotes are WT and segregate WT, DpyUncs and Unc Steriles which have an everted vulva. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. |
| GS328 |
unc-36(e251) evl-19(ar98)/dpy-17(e164) unc-32(e189) III. |
Heterozygotes are WT and segregate WT, DpyUncs and Sterile Unc-36 which have an everted vulva. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. |
| GS387 |
unc-36(e251) evl-14(ar112)/dpy-17(e164) unc-32(e189) III. |
Heterozygotes are WT and segregate WT, DpyUncs and Sterile Unc-36 which have an everted vulva. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. |
| GS422 |
evl-21(ar122) unc-36(e251)/unc-93(e1500) dpy-17(e164) III. |
Heterozygotes are WT and segregate WT, DpyUncs and Sterile Unc-36 which have an everted vulva. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. |
| GS457 |
evl-18(ar117) dpy-17(e164)/unc-36(e251) III. |
Heterozygotes are WT and segregate WT, Uncs and Sterile Dpys which have an everted vulva. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. |
| HR961 |
ced-7(n1829) dpy-17(e164) III. |
Dpy. Maternal effect persistent cell corpses. This was derived from MT4993; it was outcrossed once to get rid of an unlinked lethal. Hatching rate has improved from 10-70% to 98%. |
| IMN34 |
ced-4(n1162) dpy-17(e164) III; glt-3(bz34) IV; nuIs5 V. |
nuIs5 [glr-1::GFP + glr-1::G(alpha)s(Q227L) V + lin-15(+)] V. Reference: Tehrani N, et al. (2014) PLoS One 9(11):e113060. |