Gene Information: lag-2

Namelag-2 View on WormBase
Species C. elegans
SequenceY73C8B.4
Genetic positionV:-12.71 +/- 0.037 cM
Genomic positionV: 3188343..3190115

Strains carrying this gene

Strain Genotype Description
BC3670 dpy-18(e364)/eT1 III; lag-2(s1486) unc-46(e177)/eT1 V. Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval, mid-larval at 15C. Maintain by picking WT. Previously called let-461(s1486).
JK1219 unc-34(e315) lag-2(q387) V/nT1 [unc-?(n754) let-?] (IV;V). Heterozygotes are Unc. [Note from Kimble lab: Min Han did a complementation test on this strain and found that unc-34(e315) is lost from this strain.] Do not distribute this strain; other labs should request it from the CGC.
JK1223 lag-2(q411) V/nT1 [unc-?(n754) let-?] (IV;V). Heterozygotes are Unc. [Used to be listed in Kimble lab as unc-5(e53); lag-2(q411 dpy-11(e224)/DnT1. Needs to be checked.] Do not distribute this strain; other labs should request it from the CGC.
JK1277 lag-2(q420) V. Temperature sensitive. Maintain at 15C. Do not distribute this strain; other labs should request it from the CGC.
JK2049 qIs19 V. qIs19 [lag-2p::GFP::unc-54 3'UTR + rol-6(su1006)] V. Rollers. Integration of qEx233. GFP expression in DTCs and other defined regions. Do not distribute this strain; other labs should request it directly from the CGC.
JK2157 lag-2(q411) V; qEx319. qEx319 [lag-2::cMyc + rol-6(su1006)].  Rollers.  Extrachromosomal array carrying myc-tagged LAG-2 protein partially rescues lag-2(null).  About 70% of progeny are Lag.  Survivors are Rollers.  Reference: Crittenden et al., Mol Biol Cell. 2006 Jul;17(7):3051-61.
JK2868 qIs56 V. qIs56 [lag-2p::GFP + unc-119(+)]. Non-unc; might still have unc-119(ed3) in background. Do not distribute this strain; other labs should request it directly from the CGC.
JT37 lag-2(sa37) V. Semidominant suppressor of lin-12(d) alleles.
JT5205 lin-12(n950) III; lag-2(sa37) V. lag-2(sa37) is a semi-dominant suppressor of lin-12(n302sd).
YG1011 baf-1(gk324) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III); qIs19 V. qIs19 [lag-2p::GFP::unc-54 3'UTR + rol-6(su1006)] V. Heterozygotes are Rollers with pharyngeal GFP signal, and segregate arrested hT2 aneuploids, and non-GFP gk324 homozygotes (Sterile and Unc). All worms express lag-2p::GFP at the distal tip cells. qIs48 is an insertion of ccEx9747 with markers: myo-2::GFP expressed brightly in the pharynx throughout development, pes-10::GFP expressed in embryos, and a gut promoter driving GFP in the intestine, and is homozygous lethal.