| BC1282 |
dpy-18(e364)/eT1 III; unc-62(s472) unc-46(e177)/eT1 V. |
Heterozygotes are WT and segregate WT, Unc-36 and dead eggs. Maintain by picking WT. Unc-62 is a recessive lethal. |
| BW1739 |
+/eT1 III; unc-62(t2012) dpy-11(e224)/eT1 V. |
Heterozygotes are wild-type, and segregate WT heterozygotes, Dpy Mel (unc-62 dpy-11 homozygotes), and dead eggs (eT1 homozygotes). unc-62 dpy-11 homozygotes give only dead embryos (96%) or dead larva (4%). |
| BW2027 |
unc-62(ct344) V; yDp1 (IV;V;f). |
Animals carrying the duplication are wild-type. Animals which have lost yDp1 are Unc and give only dead eggs(57%) or dead larvae(43%). |
| CB2030 |
unc-62(e644) dpy-11(e224) V. |
UncDpy. |
| CB644 |
unc-62(e644) V. |
Unc. Recessive. Bursae abnormal. Males abnormal. M-MATING-NO SUCCESS. |
| CF1632 |
unc-62(mu232) muIs35 V. |
muIs35 [mec-7::GFP + lin-15(+)]. Egl. GFP+. QR pax migration defect. |
| CZ1072 |
unc-62(e917) V. |
Inversion which may serve as a balancer for the center of LG V. Maternal effect lethal allele which results in 57% embryonic arrest, 40% larval arrest, and 3% which survive to be fertile adults with a variety of defects including Egl, Unc and Vab. |
| SD1879 |
unc-62(e644) V; wgIs600. |
wgIs600 [unc-62::TY1::EGFP::3xFLAG(92C12) + unc-119(+)]. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence by recombineering. Derived from parental strains CB644 and SD1871. |
| SD1880 |
unc-62(s472) V; wgIs600. |
wgIs600 [unc-62::TY1::EGFP::3xFLAG(92C12) + unc-119(+)]. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence by recombineering. Derived from parental strains BC1282 and SD1871. |
| SD1887 |
unc-62(e644) V; gaIs286. |
gaIs286 [unc-62(7b)::TY1::EGFP::3xFLAG(92C12) + unc-119(+)]. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence by recombineering. A STOP-codon was inserted into exon 7a of unc-62 to generate an UNC-62(7b)-specific reporter. Recombineered fosmid was integrated by biolistic bombardment to produce strain OP602, which wa outcrossed to produce SD1894. References: Sarov, M, et al. Nat Methods (2006) 10:839-44. Gerstein MB, et al. Science. 2010 Dec 24;330(6012):1775-87. Strain was constructed as part of the Regulatory Element Project, part of modENCODE (http://www.modencode.org) Derived from parental strains BC1282 and SD1894. |
| VC3285 |
unc-62(gk3507) V/nT1 [qIs51] (IV;V). |
T28F12.2. Homozygous lethal deletion chromosome balanced by GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP gk3507 homozygotes (probable embryonic arrest). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: CGAAGCCAACACAAGAATCA. External right primer: TCGGTGTGCAAATCCAATTA. Internal left primer: ATCATCTTGCCGAAATCTGG. Internal right primer: TTTGACGTTCAGTTTGCTGG. Internal WT amplicon: 2229 bp. Deletion size: 628 bp. Deletion left flank: AGGCTTCCAATTTATTTTTTACACGCTTTT. Deletion right flank: GCGATAAATTTATCTGCAGGTCCTTTGAAG. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807 |