| BC2010 |
let-60(s1124) unc-22(s7) unc-31(e169) IV/nT1 (IV;V). |
Heterozygotes are WT and segregate WT, Vul and UncLethals. Strong allele of let-60. Unc Twitchers arrest as rod-like early larvae; rare escapers die as Vul adults. |
| BC2029 |
let-60(s1155) unc-22(s7) unc-31(e169) IV/nT1 (IV;V). |
Heterozygotes are WT and segregate WT, Vul and Twitcher UncLethals. s1155 is viable; 14% Vul. |
| BC966 |
let-60(s59) unc-22(s7)/unc-5(e152) dpy-4(e1166) IV. |
Heterozygotes are WT and segregate WT, DpyUnc, and Lethal Twitchers. Lethal mid-larval (leaky). Heterozygotes twitch in nicotine. |
| DR1172 |
let-60(s59) unc-22(s7) IV/nT1 [let-?(m435)] (IV;V). |
Heterozygotes are WT and segregate WT, Lethal Twitchers, and dead eggs. Lethal mid-larval (leaky). Maintain by picking WT. Heterozygotes twitch in 1% nicotine. |
| MH1131 |
soc-2(ku167) let-60(n1046) IV. |
Less than 5% Muv. Previously called sur-8(ku167). |
| MH231 |
let-60(n1046) IV; ksr-1(ku68) X. |
Semi-dominant suppressor of let-60(n1046). Strong reduction of function mutation. At 20C: <1% Muv, 24% Egl, 6% larval lethal, and SM migration defects. |
| MH351 |
let-60(sy101sy127)/dpy-20(e1282) IV. |
Heterozygotes are WT and segregate WT, Dpys and lethals. |
| MH538 |
mek-2(ku114) I; let-60(n1046) IV. |
|
| MT2124 |
let-60(n1046) IV. |
Non-lethal let-60 allele, multivulva phenotype (93% penetrance). Semi-Dominant (17% of hets are Muv). Amber suppressible. Non-null. PKA lin-34. See WBPaper00006902: strain probably carries a side mutation(s) that impairs chemotaxis to the odorant isoamyl alcohol. |
| MT4244 |
unc-24(e138) let-60(n1046) IV. |
Unc. Muv (sd, gf). |
| MT4574 |
let-60(n1531)/let-60(n1046) unc-22(e66) IV. |
Heterozygotes are WT and segregate WT, MuvUnc (n1046 e66) and n1531 homozygotes whose phenotypes range from a few dead larva to sterile adults. The normal larval lethality of n1531/n1531 is suppressed by the maternal contribution of n1046. n1046 is semi-dominant. |
| MT4698 |
let-60(n1700) IV. |
Muv. |
| MT4700 |
let-60(n1531) unc-22(e66)/let-60(n1700) dpy-20(e1282) IV; him-5(e1490) V. |
Heterozygotes are mostly non-Muv (about 20% will be Muv because n1700 is semi-dominant) and non-Unc. Hets segregate Twitchers which die at various larval stages or as Vul adults, because of the maternal effect rescue of n1531 by n1700. The Twitchers will not give viable progeny. Hets also segregate DpyMuvs. The strain segregates males which will mate, though not very well. |
| MT4725 |
let-60(n1046) dpy-20(e1282) IV. |
Dpy (ts). Muv (sd, gf). |
| MT4828 |
let-60(n2031)/dpy-20(e1362) unc-22(e66) IV. |
n2031 homozygotes are dead. n2031/dpy-20 unc-22 animals are Vul (27%) or WT (73%). |
| MT4866 |
let-60(n2021) IV. |
Lethal suppressor of lin-15(n765). |
| MT5449 |
clr-1(e1745) II; let-60(n2021) IV. |
Non-Lethal allele of let-60. Clear. Suppresses n765. Maintain at 15C. |
| MT5499 |
let-60(n1876) unc-22(s7) IV/nT1 [let-?(m435)] (IV;V). |
Heterozygotes are WT and segregate WT, Lethal twitchers and dead eggs. |
| MT5748 |
let-60(n2022) IV/nT1 [unc-?(n754) let-?] (IV;V). |
|
| MT5749 |
let-60(n2034) IV/nT1 [unc-?(n754) let-?] (IV;V). |
Heterozygotes are Unc and segregate Unc, Vul and dead eggs. |
| MT5750 |
let-60(n2035) IV/nT1 [unc-?(n754) let-?] (IV;V). |
|
| MT6378 |
lin-45(n2506) let-60(n1046) IV. |
n2506 suppresses n1046. |
| MT6399 |
let-60(n1046) IV; cdf-1(n2527) X. |
cdf-1(n2527) suppresses the let-60(n1046gf) Muv phenotype from 95% Muv to <5% Muv. Suppression is weakly semi-dominant. |
| MT6400 |
let-60(n1046) IV; cgr-1(n2528) X. |
Low penetrance Muv (<5%). Due to variable penetrance larval lethal phenotype at 15C, maintain at 20C. |
| NH2267 |
let-60(n1046) IV; egl-15(n1454) X. |
Unc and scrawny, but viable. Egl. Class II egl-15 mutation. Lethality suppressed by let-60. |
| NH2531 |
let-60(ay75)/dpy-20(e1362) IV. |
ay75gf: ts dominant Clr, Sterile; Dominant sterile at 25C, fertile at 15C, severely reduced fertility at 20C. At 15C, ay75gf: recessive adults Clr, partially penetrant Muv. At 15C, heterozygotes segregate 50% WT, 25% Dpy, and 25% adult Clear. |
| PJ1062 |
let-60(n1046) IV; ccIs55 V. |
ccIs55 [unc-54::lacZ + sup-7(st5)] V. |
| PJ1063 |
let-60(ga89) IV; ccIs55 V. |
ccIs55 [unc-54::lacZ + sup-7(st5)] V. Temperature sensitive. Nearly WT at 15C. At 20C the animals are 18% Muv and brood size is 88. At 25C the animals are 57% Muv and are almost sterile (brood size is 6). |
| PJ1065 |
let-60(n2021) IV; ccIs55 V. |
ccIs55 [unc-54::lacZ + sup-7(st5)] V. Vul/Egl. ras loss-of-signal. |
| PJ1069 |
let-60(sy93) IV; ccIs55 V. |
ccIs55 [unc-54::lacZ + sup-7(st5)] V. Dominant Vul allele, however, worms appear to be Egl and have multiple pseudovulvae (due to sup-7??). |
| PJ1077 |
let-60(ga89) IV; lwIs16 X. |
lwIs16 [act-4::lacZ] X. Temperature sensitive gain-of-function allele of ras. At high temperatures worms become Clr. Should also become Muv- not noted. Maintain at 16C. |
| PJ1093 |
let-60(ga89) IV; ccIs55 V; gap-1(n1329) X. |
ccIs55 [unc-54::lacZ + sup-7(st5)] V. Strain is viable at 15-25C. let-60(ga89) is temperature sensitive; however, with the gap-1 in the background the animals still appear somewhat Clr and Muv even at low temperatures. |
| PJ1099 |
lin-45(sy96) let-60(ga89) IV; ccIs55 V. |
ccIs55 [unc-54::lacZ + sup-7(st5)] V. Vul. Non-Clr at 25C. Poor growers (sub-viable?) at 25C. |
| PJ1100 |
him-1(e879) I; let-60(ga89) IV; ccIs55 V. |
ccIs55 [unc-54::lacZ + sup-7(st5)] V. Temperature sensitive. Nearly WT at 15C. At 20C the animals are 18% Muv and brood size is 88. At 25C the animals are 57% Muv and almost sterile (brood size is 6). Males appear to mate poorly - not quantitatively measured but very poor success with matings. |
| PJ1105 |
mek-2(ku114) I; let-60(ga89) IV; ccIs55 V. |
ccIs55 [unc-54::lacZ + sup-7(st5)] V. Occasional bags and L1 lethality. ga89 is temperature sensitive. Maintain at 16C. |
| PJ1107 |
soc-2(n1774) let-60(ga89) IV; ccIs55 V. |
ccIs55 [unc-54::lacZ + sup-7(st5)] V. Appear to have DV and bags with some frequencey. Appear to give Clr phenotype at 16C. Occasional Muv seen. Very frequently sterile due to lack of gonad development. Maintain at 16C. |
| PJ1109 |
mpk-1(n2521) III; let-60(ga89) IV; ccIs55 V. |
ccIs55 [unc-54::lacZ + sup-7(st5)] V. |
| PS436 |
let-60(sy93) IV. |
Dominant Vul (>99% Egl). Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. |
| PS4627 |
let-60(n1046) unc-31(e169) V/nT1 [let(m435)] (IV;V). |
Maintain by picking WT. Segregates Muv Unc (let-60 unc-31 homozygotes), wild-type heterozygotes and dead eggs. Reference: Moghal N, Sternberg PW. Development. 2003 Jan;130(1):57-69. |
| PS468 |
let-60(sy100) dpy-20(e1282)/let-60(n1046) unc-22(s7) IV; him-5(e1490) V. |
Heterozygotes are weak Muv and segregate weak Muv, Muv Twitchers, and Dpy Vuls whose progeny are larval lethal. sy100 is a dominant negative allele of let-60. n1046 is a gain-of-function allele of let-60. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. |
| PS524 |
let-60(sy100) dpy-20(e1282) IV/nT1 [let-?(m435)] (IV;V). |
Heterozygotes are non-Dpy Vul and segregate non-Dpy Vul, DpyVul whose progeny are dead, and dead eggs. sy100 is dominant Vul and recessive Lethal with maternal rescue: homozygotes from heterozygous mothers grow to adulthood and become a bag of dead larvae. sy100 is not 100% penetrant. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. |
| PS536 |
unc-24(e138) let-60(sy99) IV/nT1 [let-?(m435)] (IV;V). |
Heterozygotes are Vul (97% Egl). Segregates dead eggs. sy99 homozygotes are lethal. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. |
| PS538 |
unc-24(e138) let-60(sy92) IV/nT1 [let-?(m435)] (IV;V). |
Heterozygotes are Vul and segregate Vul, Unc-24 lethals and dead eggs. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. |
| SD366 |
mpk-1(n2521) III; let-60(n1046) IV. |
Less than 5% Muv animals. |
| SD551 |
let-60(ga89) IV. |
Temperature sensitive. Nearly WT at 15C. At 20C the animals are 18% Muv and brood size is 88. At 25C the animals are 57% Muv and almost sterile (brood size is 6). Maintain at 15C. |
| VC1447 |
let-60(ok1932) IV/nT1 [qIs51] (IV;V). |
ZK792.6. Homozygous lethal deletion chromosome balanced by GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP ok1932 homozygotes (late larval arrest). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: GCAATACGAGGGAGCAAAAA. External right primer: ACTCATTCCGACTCACCACC. Internal left primer: AAATGAGTTGGCGAAATTGG. Internal right primer: CCCACAGAAATCCTTCTCCA. Internal WT amplicon: 2164 bp. Deletion size: 992 bp. Deletion left flank: CCTTAATTGTACATTAAAAACATTATTTTT. Deletion right flank: TTCGTCTTTGGAATCAATTAAACAGCTCGA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807 |
| WU684 |
let-60(n1046) IV; cgr-1(am114) X. |
Low penetrance Muv (<1%). Slow growing at 20C. 75% larval lethality at 15C. Maintain at 20C. |