| BB21 |
adr-1(tm668) I; adr-2(ok735) III. |
Reduced lifespan, chemotaxis defective, co-suppression of transgenes in somatic cells. Maintain under normal conditions. Reference: Hundley HA, et al. RNA. 2008 Oct;14(10):2050-60. |
| BB22 |
adr-2(gv42) rde-4(ne299) III. |
RNAi deficient. Maintain under normal conditions. |
| BB23 |
adr-1(gv6) I; adr-2(gv42) III; rde-1(ne219) V. |
RNAi deficient. Maintain under normal conditions. Reference: Tonkin LA & BassBL. Science. 2003 Dec 5;302(5651):1725. Knight SW & Bass BL. Mol Cell. 2002 Oct;10(4):809-17. |
| BB239 |
adr-1(uu49) I; adr-2(uu28) III. |
Chemotaxis deficient. Transgenes are silenced in this background. Reference: Reich DP, et al. Genes Dev. 2018 Feb 1;32(3-4):271-282. NOTE: In the referenced publication, irregularities were noted in the adr-1(gv6);adr-2(gv42) null strain, which were ascribed to background mutationsint hat strain. This strain -- adr-1(uu49);adr-2(uu28) -- was generated Crispr/Cas9 targeted mutation and phenotypes are more consistent with another null strain, adr-1(tm668);adr-2(ok735). |
| BB24 |
adr-1(gv6) I; adr-2(gv42) rde-4(ne299) III. |
RNAi deficient. Maintain under normal conditions. Reference: Tonkin LA & BassBL. Science. 2003 Dec 5;302(5651):1725. |
| BB242 |
adr-1(uu49) I; adr-2(uu28) III; rde-1(uu51) V. |
RNAi deficient. Lacks RNA editing. Reference: Reich DP, et al. Genes Dev. 2018 Feb 1;32(3-4):271-282. |
| BB244 |
adr-1(uu49) I; adr-2(uu28) rde-4(uu53) III. |
RNAi deficient. Lacks RNA editing. Reference: Reich DP, et al. Genes Dev. 2018 Feb 1;32(3-4):271-282. |
| BB259 |
adr-1(uu49) I; adr-2(uu28) III; ggIs1 IV. |
ggIs1 [nrde-3p::3xFlag::GFP::nrde-3 ORF + unc-119(+)] IV. Nuclear GFP::NRDE-3 signal. Lacks RNA editing. Reference: Reich DP, et al. Genes Dev. 2018 Feb 1;32(3-4):271-282. |
| BB261 |
adr-1(uu49) I; rrf-3(uu56) II; adr-2(uu28) III. |
Enhanced RNAi. Lacks RNA editing. Vulval bursting. Low brood size. Triple mutants display bursting and low brood size phenotypes not observed in adr-1;adr-2 or rrf-3 parental strains. Reference: Reich DP, et al. Genes Dev. 2018 Feb 1;32(3-4):271-282. |
| BB270 |
adr-1(uu49) I; rrf-3(uu56) II; adr-2(uu28) III; rde-1(uu51) V. |
RNAi deficient. Lacks RNA editing. Reference: Reich DP, et al. Genes Dev. 2018 Feb 1;32(3-4):271-282. |
| BB272 |
adr-1(uu49) I; rrf-3(uu56) II; adr-2(uu28) rde-4(uu53) III. |
RNAi deficient. Lacks RNA editing. Reference: Reich DP, et al. Genes Dev. 2018 Feb 1;32(3-4):271-282. |
| BB278 |
adr-1(uu49) I; rrf-3(uu56) II; adr-2(uu28) III; ggIs1 IV. |
ggIs1 [nrde-3p::3xFlag::GFP::nrde-3 ORF + unc-119(+)] IV. Nuclear GFP::NRDE-3 signal. Enhanced RNAi. Lacks RNA editing. Vulval bursting. Low brood size. Triple mutants display bursting and low brood size phenotypes not observed in adr-1;adr-2 or rrf-3 parental strains. Reference: Reich DP, et al. Genes Dev. 2018 Feb 1;32(3-4):271-282. |
| BB283 |
adr-1(uu49) I; adr-2(uu28) III; ergo-1(uu68) V. |
Enhanced RNAi. Lacks RNA editing. Vulval bursting. Low brood size. Triple mutants display bursting and low brood size phenotypes not observed in adr-1;adr-2 or rrf-3 parental strains. Reference: Reich DP, et al. Genes Dev. 2018 Feb 1;32(3-4):271-282. |
| BB3 |
adr-2(gv42) III. |
Defective chemotaxis to volatile odorant. |
| BB4 |
adr-1(gv6) I; adr-2(gv42) III. |
Defective chemotaxis to volatile odorant. Low percentage (about 6%) have protruding vulva. |
| RB886 |
adr-2(ok735) III. |
T20H4.4. Homozygous. Outer Left Sequence: CTCCATATTCGCTTCCGTGT. Outer Right Sequence: AGAACACGCTCTTCGTCGAT. Inner Left Sequence: CACGATGCTGCATGAGATTT. Inner Right Sequence: AGCTCGCTTCCAATCTTCAA. Inner primer WT PCR product: 2144. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807 |