| BA824 |
spe-26(hc139) dpy-20(e1282) IV. |
Temperature sensitive. Partially fertile at 16C (very few progeny). Sterile at 20C and 25C. Weak Dpy at 15C. Spermatogenesis arrests at the spermatocyte stage. |
| BA825 |
spe-26(hc140) dpy-20(e1282)/+ IV. |
Heterozygotes are WT and segregate wild-type, wild-type heterozygotes, and Sterile Dpy (spe-4 dpy-20 homozygotes). Homozygous mutants are weak Dpy and partially fertile at 15C. Sterile at 20-25C. Spermatogenesis arrests at the spermatocyte stage. |
| BA901 |
spe-27(it110) dpy-20(e1282) IV. |
Temperature sensitive Dpy. Male/hermaphrodite mating strain. Hermaphrodites self-sterile at all temps; males fertile. Spermatids arrest with spikes in pronase; form normal pseudopods in TEA. |
| BA925 |
spe-26(hc138) dpy-20(e1282) IV. |
Temperature-sensitive. Fertile and weak Dpy at 15C. Partially fertile at 20C. Sterile at 25C. Twitcher. Spermatogenesis arrests at the spermatocyte stage. |
| BA959 |
spe-29(it127) dpy-20(e1282) IV. |
Homozygous male/hermaphrodite line. Males are fertile. Hermaphrodites are sterile, but slightly leaky producing a few progeny (at 25C - ts not tested). In pronase, spermatids from males activate to form many long spikes, terminating at this stage. A very few (1-3 per worm) activate to form normal-looking, motile spermatozoons. |
| BA969 |
spe-6(hc163) III; spe-27(it132) dpy-20(e1282) IV. |
Dpy. spe-6(hc163) is a recessive suppressor of spe-27(it132). spe-6(hc163) also suppresses spe-8, spe-12, spe-29 and other spe-27 alleles. Causes precocious spermatid activation. Fertile between 15-25C. |
| BA971 |
spe-27(it132) dpy-20(e1282) IV. |
Temperature sensitive spe-27 allele. Leaky sterile at 20C. Males at 20C produce spermatids that form spikes in pronase. Males are fertile. Temperature sensitive dpy-20 allele. Maintain at 15C. |
| BA975 |
spe-6(hc163) III; spe-29(it127) dpy-20(e1282) IV. |
spe-6(hc163) suppresses the self-sterility of spe-29 in this strain. Self-sterile at 25C. |
| BT14 |
fbl-1(hd43)/dpy-20(e1282) unc-24(e138) IV. |
Heterozygotes are WT and segregate WT, Steriles (hd43 homozygotes) and Dpy Uncs. |
| CB1282 |
dpy-20(e1282) IV. |
Dpy. Temperature sensitive. Lethal cold sensitive. Male lethal. M-MATING++ 1-10%WT. |
| CB3775 |
dpy-20(e2017) IV. |
Severe roundheaded Dpy. Almost inviable at 15C. |
| CB3823 |
eDf18/unc-24(e138) dpy-20(e1282) IV. |
Heterozygotes are WT and segregate WT, DpyUnc and dead eggs. Maintain by picking WT. |
| CB3824 |
eDf19/unc-24(e138) dpy-20(e1282) IV. |
Heterozygotes are WT (somewhat Unc and Egl) and segregate WT, DpyUnc and dead eggs. Maintain by picking WT. |
| CB3843 |
fem-3(e1996)/unc-24(e138) dpy-20(e1282) IV. |
Heterozygotes are WT and segregate WT, DpyUnc and fertile females. |
| CB3874 |
dpy-20(e2017) IV; sup-28(e2058) X. |
Partly or completely suppressed Dpy. |
| CB3875 |
sup-23(e2059) dpy-20(e2017) IV. |
Weak suppression of dpy-20 by sup-23. Medium Dpy. |
| CB3908 |
sup-22(e2057) dpy-20(e2017) IV. |
Weak suppression of dpy-20 by sup-22. Dpy phenotype, but less Dpy than dpy-20 alone. |
| CB3909 |
dpy-20(e2017) IV; sup-21(e1957) X. |
Suppressed Dpy. e1957 previously called sup-21. |
| CB4227 |
sup-34(e2227) I; unc-24(e138) dpy-20(e2017) IV. |
sup-34 is dominant amber suppressor: weak suppression of Unc, medium suppression of Dpy. |
| CB4435 |
dpy-20(e2017) sup-24(st354) IV. |
Extreme Dpy suppressed to weak Dpy or non-Dpy phenotype by amber suppressor. Received new stock from J. Hodgkin 5/31/95. |
| CB4747 |
dpy-20(e2017) unc-24(e138) IV; sup-33(st389) X. |
Variable phenotypes, slightly dumpy and/or uncoordinated, especially at 25C. Grows poorly at 15C. Low fertility. sup-33(st389) is a weak amber suppressor; e2017 and e138 are amber mutations. Reference: Kondo K, et al. (1990) J Mol Biol. 1990 Sep 5;215(1):7-19. |
| CB4760 |
fem-1(e2044) mor-2(e1125) unc-24(e158) fem-3(q20) / unc-5(e53) dpy-20(e1282) IV |
Wild-type hermaphrodites segregating wild-type hermaphrodites, Unc-24 females, Unc Dpy hermaphrodites. Maintain by picking wild-type hermaphrodites. Deletion allele of fem-1, with unusual maternal effect. Reference: Spence AM, et al. Cell. 1990 Mar 23;60(6):981-90. Johnson CL & Spence AM.. Science. 2011 Sep 2;333(6047):1311-4. |
| CF439 |
lin-39(mu26) III; dpy-20(e1282) IV; him-5(e1490) V; muIs23. |
muIs23 [hsp::lin-39 + (pMH86) dpy-20(+)]. Heat-shock inducible lin-39. muIs23 is a spontaneous integrant whose chromosomal location is unknown. [NOTE: This strain was previously described as carrying lin-39(n1760), but it is actually carrying the g to a substitution of mu26. Strain MT7255 has been confirmed to be carrying the a to t substitution of n1760.] |
| CF453 |
muIs16 II; dpy-20(e1282) IV. |
muIs16 [mab-5::GFP + dpy-20(+)]. non-Dpy. |
| CF579 |
dpy-20(e1282) IV; him-5(e1490) V; muIs27. |
muIs27 [mig-2::GFP + dpy-20(+)]. Him. non-Dpy. GFP is membrane enriched and expressed in many cells throughout development (see reference for details). Not known in which LG muIs27 is integrated. |
| CF716 |
dpy-20(e1282) IV; mig-13(mu31) X; muIs37. |
muIs37 [(pMS114) hsp::mig-13 + (pMH86) dpy-20(+)]. Inducible heat-shock promoter driven mig-13 rescues Q cell migration defects in mig-13(mu31) mutants. Reference: Sym, M., Robinson, N., and Kenyon, C. Cell. 1999 Jul 9;98(1):25-36. |
| CF891 |
dpy-20(e1282) IV; muIs37. |
muIs37 [(pMS114) hsp::mig-13 + (pMH86) dpy-20(+)]. Inducible heat-shock promoter driven mig-13 rescues Q cell migration defects in mig-13(mu31) mutants. Reference: Sym, M., Robinson, N., and Kenyon, C. Cell. 1999 Jul 9;98(1):25-36. |
| CF896 |
dpy-20(e1282) IV; muIs42. |
muIs42[mig-13::GFP + dpy-20(+)]. non-Dpy strain. GFP+ in ventral cord cells, pharyngeal-intestinal valve cells. |
| DA449 |
eat-1(e2343) dpy-20(e1282) IV. |
Thin, starved, healthy adults. Retain few eggs. Slow irregular pumping at 16, 20, 25C. Dpy. |
| DA469 |
dpy-20(e1282) unc-31(e928) IV. |
Dpy (ts). Unc. |
| DA491 |
dpy-20(e1282) unc-30(e191) IV. |
Unc. Temperature sensitive Dpy. |
| DL220 |
dpy-20(e1282) mig-32(tm1684) IV. |
|
| DR105 |
unc-17(e245) dpy-20(e1282) IV. |
DpyUnc. |
| DR439 |
unc-8(e49) dpy-20(e1282) IV. |
DpyUnc. |
| ET263 |
ddb-1(tm1769)/dpy-20(e2017) IV. |
Heterozygotes are WT and segregate WT, Dpy and ddb-1 homozygotes, which arrest as L2-stage larvae (approx. 15%) or become sterile adults with protruding vulva (approx. 85%). |
| EW55 |
dpy-20(e1362) IV; deEx100. |
deEx100 [ajm-1::GFP + HinfI digested complex DNA + lin-44p::4xNLS::GFP + dpy20(+)]. Pick non-Dpy to maintain. |
| EW56 |
dpy-20(e1362) IV; deEx101. |
deEx101[dpy20(+); ajm-1::GFP; HinfI digested complex DNA; cwn-1p::4xNLSGFP]. Pick non-Dpy to maintain. |
| EW57 |
dpy-20(e1362) IV; deEx102. |
deEx102[dpy20(+); ajm-1::GFP; HinfI digested complex DNA; egl-20p::4xNLSGFP]. Pick non-Dpy to maintain. |
| EW58 |
dpy-20(e1362) IV; deEx103. |
deEx103[dpy20(+); ajm-1::GFP; HinfI digested complex DNA; cwn-2p::4xNLSGFP]. Pick non-Dpy to maintain. |
| EW59 |
dpy-20(e1362) IV; deEx104. |
deEx104 [ajm-1::GFP + mom-2p::4xNLS::GFP + dpy20(+) + HinfI digested complex DNA]. Pick non-Dpy to maintain. |
| EW61 |
dpy-20(e1282) IV; him-5(e1490) V; deIs4. |
deIs4 [ajm-1::GFP + GFP::lin-39(YAC) + dpy-20(+)]. A transcriptional fusion of GFP to the ATG of lin-39, with a stop codon between GFP and LIN-39 sequences. Present on a YAC. Yeast chromosomal DNA was injected and integrated. Expression is very faint and may get fainter over time. Freeze upon receipt. |
| EW62 |
smg-1(e1228) I; dpy-20(e1282) IV; him-5(e1490) V; deIs4. |
deIs4 [ajm-1::GFP + GFP::lin-39(YAC) + dpy-20(+)]. A transcriptional fusion of GFP to the ATG of lin-39, with a stop codon between GFP and LIN-39 sequences. Present on a YAC. Yeast chromosomal DNA was injected and integrated. Expression is very faint and may get fainter over time. Therefore, freeze upon receipt. |
| FX19161 |
dpy-20(tm5940)/tmIn5 IV. |
Homozygous lethal or sterile deletion allele balanced by Unc-marked translocation. Break points: In(mec-3 unc-31) IV. Covered region (Mb) 2.3 (10.5..12.8) Unc. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. |
| GS1826 |
dpy-20(e1282) IV; arIs36. |
arIs36 [(pJF43) hsp::ssGFP + dpy-20(+) + pBluescript SK+]. GFP is secreted into the pseudocoelom upon heat shock and is taken up by coelomocytes. Not known where arIs36 is attached. Do not distribute this strain; other labs should request it from the CGC. |
| GS1912 |
arIs37 I; dpy-20(e1282) IV. |
arIs37 [myo-3p::ssGFP + dpy-20(+)] I. GFP is secreted from muscle cells. Do not distribute this strain; other labs should request it from the CGC. |
| GS2477 |
arIs37 I; cup-5(ar465) III; dpy-20(e1282) IV. |
arIs37 [myo-3p::ssGFP + dpy-20(+)] I. cup-5 is defective in endocytosis by coelomocytes. Do not distribute this strain; other labs should request it from the CGC. |
| GS2478 |
arIs37 I; dpy-20(e1282) IV; cup-8(ar466) V. |
arIs37 [myo-3p::ssGFP + dpy-20(+)] I. cup-8 is defective in endocytosis by coelomocytes. Do not distribute this strain; other labs should request it from the CGC. |
| GS2479 |
arIs37 I; dpy-20(e1282) IV; cup-9(ar467) V. |
arIs37 [myo-3p::ssGFP + dpy-20(+)] I. cup-9 is defective in endocytosis by coelomocytes. Do not distribute this strain; other labs should request it from the CGC. |
| GS2484 |
arIs37 I; dpy-20(e1282) IV; cup-11(ar472) X. |
arIs37 [myo-3p::ssGFP + dpy-20(+)] I. cup-11 is defective in endocytosis by coelomocytes. Do not distribute this strain; other labs should request it from the CGC. |
| GS2495 |
arIs37 I; dpy-20(e1282) IV; mtm-9(ar479) V. |
arIs37 [myo-3p::ssGFP + dpy-20(+)] I. Coelomocyte endocytosis defect. GFP accumulates in body cavity. MTM-9=Y39H10A.3 mtm-9 pka cup-10. Do not distribute this strain; other labs should request it from the CGC. |
