Gene Information: dpy-20

Namedpy-20 View on WormBase
Species C. elegans
SequenceT22B3.1
Genetic positionIV:5.22 +/- 0.004 cM
Genomic positionIV: 11696430..11700852

Strains carrying this gene

Strain Genotype Description
BA824 spe-26(hc139) dpy-20(e1282) IV. Temperature sensitive. Partially fertile at 16C (very few progeny). Sterile at 20C and 25C. Weak Dpy at 15C. Spermatogenesis arrests at the spermatocyte stage.
BA825 spe-26(hc140) dpy-20(e1282)/+ IV. Heterozygotes are WT and segregate wild-type, wild-type heterozygotes, and Sterile Dpy (spe-4 dpy-20 homozygotes). Homozygous mutants are weak Dpy and partially fertile at 15C. Sterile at 20-25C. Spermatogenesis arrests at the spermatocyte stage.
BA901 spe-27(it110) dpy-20(e1282) IV. Temperature sensitive Dpy. Male/hermaphrodite mating strain. Hermaphrodites self-sterile at all temps; males fertile. Spermatids arrest with spikes in pronase; form normal pseudopods in TEA.
BA925 spe-26(hc138) dpy-20(e1282) IV. Temperature-sensitive. Fertile and weak Dpy at 15C. Partially fertile at 20C. Sterile at 25C. Twitcher. Spermatogenesis arrests at the spermatocyte stage.
BA959 spe-29(it127) dpy-20(e1282) IV. Homozygous male/hermaphrodite line. Males are fertile. Hermaphrodites are sterile, but slightly leaky producing a few progeny (at 25C - ts not tested). In pronase, spermatids from males activate to form many long spikes, terminating at this stage. A very few (1-3 per worm) activate to form normal-looking, motile spermatozoons.
BA969 spe-6(hc163) III; spe-27(it132) dpy-20(e1282) IV. Dpy. spe-6(hc163) is a recessive suppressor of spe-27(it132). spe-6(hc163) also suppresses spe-8, spe-12, spe-29 and other spe-27 alleles. Causes precocious spermatid activation. Fertile between 15-25C.
BA971 spe-27(it132) dpy-20(e1282) IV. Temperature sensitive spe-27 allele. Leaky sterile at 20C. Males at 20C produce spermatids that form spikes in pronase. Males are fertile. Temperature sensitive dpy-20 allele. Maintain at 15C.
BA975 spe-6(hc163) III; spe-29(it127) dpy-20(e1282) IV. spe-6(hc163) suppresses the self-sterility of spe-29 in this strain. Self-sterile at 25C.
BT14 fbl-1(hd43)/dpy-20(e1282) unc-24(e138) IV. Heterozygotes are WT and segregate WT, Steriles (hd43 homozygotes) and Dpy Uncs.
CB1282 dpy-20(e1282) IV. Dpy. Temperature sensitive. Lethal cold sensitive. Male lethal. M-MATING++ 1-10%WT.
CB3775 dpy-20(e2017) IV. Severe roundheaded Dpy. Almost inviable at 15C.
CB3823 eDf18/unc-24(e138) dpy-20(e1282) IV. Heterozygotes are WT and segregate WT, DpyUnc and dead eggs. Maintain by picking WT.
CB3824 eDf19/unc-24(e138) dpy-20(e1282) IV. Heterozygotes are WT (somewhat Unc and Egl) and segregate WT, DpyUnc and dead eggs. Maintain by picking WT.
CB3843 fem-3(e1996)/unc-24(e138) dpy-20(e1282) IV. Heterozygotes are WT and segregate WT, DpyUnc and fertile females.
CB3874 dpy-20(e2017) IV; sup-28(e2058) X. Partly or completely suppressed Dpy.
CB3875 sup-23(e2059) dpy-20(e2017) IV. Weak suppression of dpy-20 by sup-23. Medium Dpy.
CB3908 sup-22(e2057) dpy-20(e2017) IV. Weak suppression of dpy-20 by sup-22. Dpy phenotype, but less Dpy than dpy-20 alone.
CB3909 dpy-20(e2017) IV; sup-21(e1957) X. Suppressed Dpy. e1957 previously called sup-21.
CB4227 sup-34(e2227) I; unc-24(e138) dpy-20(e2017) IV. sup-34 is dominant amber suppressor: weak suppression of Unc, medium suppression of Dpy.
CB4435 dpy-20(e2017) sup-24(st354) IV. Extreme Dpy suppressed to weak Dpy or non-Dpy phenotype by amber suppressor. Received new stock from J. Hodgkin 5/31/95.
CB4747 dpy-20(e2017) unc-24(e138) IV; sup-33(st389) X. Variable phenotypes, slightly dumpy and/or uncoordinated, especially at 25C. Grows poorly at 15C. Low fertility. sup-33(st389) is a weak amber suppressor; e2017 and e138 are amber mutations. Reference: Kondo K, et al. (1990) J Mol Biol. 1990 Sep 5;215(1):7-19.
CB4760 fem-1(e2044) mor-2(e1125) unc-24(e158) fem-3(q20) / unc-5(e53) dpy-20(e1282) IV Wild-type hermaphrodites segregating wild-type hermaphrodites, Unc-24 females, Unc Dpy hermaphrodites. Maintain by picking wild-type hermaphrodites. Deletion allele of fem-1, with unusual maternal effect. Reference: Spence AM, et al. Cell. 1990 Mar 23;60(6):981-90. Johnson CL & Spence AM.. Science. 2011 Sep 2;333(6047):1311-4.
CF439 lin-39(n1760) III; dpy-20(e1282) IV; him-5(e1490) V; muIs23. muIs23 [hsp::lin-39 + (pMH86) dpy-20(+)]. Heat-shock inducible lin-39. muIs23 is a spontaneous integrant whose chromosomal location is unknown.
CF453 muIs16 II; dpy-20(e1282) IV. muIs16 [mab-5::GFP + dpy-20(+)]. non-Dpy.
CF579 dpy-20(e1282) IV; him-5(e1490) V; muIs27. muIs27 [mig-2::GFP + dpy-20(+)]. Him. non-Dpy. GFP is membrane enriched and expressed in many cells throughout development (see reference for details). Not known in which LG muIs27 is integrated.
CF716 dpy-20(e1282) IV; mig-13(mu31) X; muIs37. muIs37 [(pMS114) hsp::mig-13 + (pMH86) dpy-20(+)]. Inducible heat-shock promoter driven mig-13 rescues Q cell migration defects in mig-13(mu31) mutants. Reference: Sym, M., Robinson, N., and Kenyon, C. Cell. 1999 Jul 9;98(1):25-36.
CF891 dpy-20(e1282) IV; muIs37. muIs37 [(pMS114) hsp::mig-13 + (pMH86) dpy-20(+)]. Inducible heat-shock promoter driven mig-13 rescues Q cell migration defects in mig-13(mu31) mutants. Reference: Sym, M., Robinson, N., and Kenyon, C. Cell. 1999 Jul 9;98(1):25-36.
CF896 dpy-20(e1282) IV; muIs42. muIs42[mig-13::GFP + dpy-20(+)]. non-Dpy strain. GFP+ in ventral cord cells, pharyngeal-intestinal valve cells.
DA449 eat-1(e2343) dpy-20(e1282) IV. Thin, starved, healthy adults. Retain few eggs. Slow irregular pumping at 16, 20, 25C. Dpy.
DA469 dpy-20(e1282) unc-31(e928) IV. Dpy (ts). Unc.
DA491 dpy-20(e1282) unc-30(e191) IV. Unc. Temperature sensitive Dpy.
DL220 dpy-20(e1282) mig-32(tm1684) IV.
DR105 unc-17(e245) dpy-20(e1282) IV. DpyUnc.
DR439 unc-8(e49) dpy-20(e1282) IV. DpyUnc.
ET263 ddb-1(tm1769)/dpy-20(e2017) IV. Heterozygotes are WT and segregate WT, Dpy and ddb-1 homozygotes, which arrest as L2-stage larvae (approx. 15%) or become sterile adults with protruding vulva (approx. 85%).
EW55 dpy-20(e1362) IV; deEx100. deEx100 [ajm-1::GFP + HinfI digested complex DNA + lin-44p::4xNLS::GFP + dpy20(+)]. Pick non-Dpy to maintain.
EW56 dpy-20(e1362) IV; deEx101. deEx101[dpy20(+); ajm-1::GFP; HinfI digested complex DNA; cwn-1p::4xNLSGFP]. Pick non-Dpy to maintain.
EW57 dpy-20(e1362) IV; deEx102. deEx102[dpy20(+); ajm-1::GFP; HinfI digested complex DNA; egl-20p::4xNLSGFP]. Pick non-Dpy to maintain.
EW58 dpy-20(e1362) IV; deEx103. deEx103[dpy20(+); ajm-1::GFP; HinfI digested complex DNA; cwn-2p::4xNLSGFP]. Pick non-Dpy to maintain.
EW59 dpy-20(e1362) IV; deEx104. deEx104 [ajm-1::GFP + mom-2p::4xNLS::GFP + dpy20(+) + HinfI digested complex DNA]. Pick non-Dpy to maintain.
EW61 dpy-20(e1282) IV; him-5(e1490) V; deIs4. deIs4 [ajm-1::GFP + GFP::lin-39(YAC) + dpy-20(+)]. A transcriptional fusion of GFP to the ATG of lin-39, with a stop codon between GFP and LIN-39 sequences. Present on a YAC. Yeast chromosomal DNA was injected and integrated. Expression is very faint and may get fainter over time. Freeze upon receipt.
EW62 smg-1(e1228) I; dpy-20(e1282) IV; him-5(e1490) V; deIs4. deIs4 [ajm-1::GFP + GFP::lin-39(YAC) + dpy-20(+)]. A transcriptional fusion of GFP to the ATG of lin-39, with a stop codon between GFP and LIN-39 sequences. Present on a YAC. Yeast chromosomal DNA was injected and integrated. Expression is very faint and may get fainter over time. Therefore, freeze upon receipt.
FX19161 dpy-20(tm5940)/tmIn5 IV. Homozygous lethal or sterile deletion allele balanced by Unc-marked translocation. Break points: In(mec-3 unc-31) IV. Covered region (Mb) 2.3 (10.5..12.8) Unc. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241.
GS1826 dpy-20(e1282) IV; arIs36. arIs36 [(pJF43) hsp::ssGFP + dpy-20(+) + pBluescript SK+]. GFP is secreted into the pseudocoelom upon heat shock and is taken up by coelomocytes. Not known where arIs36 is attached. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
GS1912 arIs37 I; dpy-20(e1282) IV. arIs37 [myo-3p::ssGFP + dpy-20(+)] I. GFP is secreted from muscle cells. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
GS2477 arIs37 I; cup-5(ar465) III; dpy-20(e1282) IV. arIs37 [myo-3p::ssGFP + dpy-20(+)] I. cup-5 is defective in endocytosis by coelomocytes. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
GS2478 arIs37 I; dpy-20(e1282) IV; cup-8(ar466) V. arIs37 [myo-3p::ssGFP + dpy-20(+)] I. cup-8 is defective in endocytosis by coelomocytes. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
GS2479 arIs37 I; dpy-20(e1282) IV; cup-9(ar467) V. arIs37 [myo-3p::ssGFP + dpy-20(+)] I. cup-9 is defective in endocytosis by coelomocytes. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
GS2484 arIs37 I; dpy-20(e1282) IV; cup-11(ar472) X. arIs37 [myo-3p::ssGFP + dpy-20(+)] I. cup-11 is defective in endocytosis by coelomocytes. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
GS2495 arIs37 I; dpy-20(e1282) IV; mtm-9(ar479) V. arIs37 [myo-3p::ssGFP + dpy-20(+)] I. Coelomocyte endocytosis defect. GFP accumulates in body cavity. MTM-9=Y39H10A.3 mtm-9 pka cup-10. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.