Gene Information: vab-7

Namevab-7 View on WormBase
Species C. elegans
SequenceM142.4
Genetic positionIII:5.60 +/- 0.012 cM
Genomic positionIII: 10920344..10926041

Strains carrying this gene

Strain Genotype Description
BA1013 spe-6(hc49) vab-7(e1562)/qC1 [dpy-19(e1259) glp-1(q339)] III; spe-27(it132) IV. Male/hermaphrodite line. Maintain at 15C to insure maintenance of male/hermaphrodite line.
BA609 spe-6(hc49) vab-7(e1562) III; eDp6 (III;f). Animals with the Duplications are WT. Animals which have lost the Duplication are Sterile and have an abnormal tail.
CB1562 vab-7(e1562) III. Unc. Tail abnormalities-twisted or knobbed. Recessive. M-MATING-NO SUCCESS.
DA438 bli-4(e937) I; rol-6(e187) II; daf-2(e1368) vab-7(e1562) III; unc-31(e928) IV; dpy-11(e224) V; lon-2(e678) X. Linkage mapping strain. Maintain at 15C.
GE42 vab-7(e1562) pha-1(e2123) III. Temperature sensitive. Maintain at 15C. pha-1(e2123) is embryonic lethal at 25C. Hermaphrodites have an abnormal tail: sometimes twisted, tail whip never formed, often bobbed; sometimes uncoordinated with bent tail. Adult male tail deformed also.
HR492 +/hT2 I; vab-7(e1562) mel-44(sb44)/hT2 [bli-4(e937) let-?(h661)] III. Dominant ts maternal-effect embryonic lethal. Embryos arrest prior to morphogenesis or at two-fold. Non-ts recessive mid-larval lethal. Maintain at 15C. Freezes poorly.
OH15912 vab-7(ot959[vab-7::GFP::FLAG]) III. Superficially wild-type. Insertion of GFP and FLAG tags directly before the stop codon of endogenous vab-7 locus. Verified by sequencing. Reference: Reilly MB, et al. Nature. 2020 Aug;584(7822):595-601. PMID: 32814896.
RW12267 vab-7(st12267[vab-7::TY1::EGFP::3xFLAG]) III. CRISPR/Cas9 engineered tagged endogneous locus.
VC1611 +/mT1 II; vab-7(gk709)/mT1 [dpy-10(e128)] III. M142.4. Apparent homozygous lethal deletion chromosome balanced by dpy-10-marked translocation. Heterozygotes are WT, and segregate WT, arrested mT1 aneuploids, sterile Dpys (mT1 homozygotes), and gk709 homozygotes (arrest stage/phenotype undetermined). Pick WT and check for correct segregation of progeny to maintain. External left primer: CCTTCCCCTCTTCTATTGGC. External right primer: CGACACGCAACACCAATTAC. Internal left primer: AATCCACCGTAGTCACCGAG. Internal right primer: TCAGAGCATGTTTCCCAGTG. Internal WT amplicon: 2427 bp. Deletion size: 1019 bp. Deletion left flank: TAAATTTTTGTAGTTTTTTAGGGATTTTTT. Deletion right flank: TCACTTGTGGAGATGGTCGCCGCATCTCGA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
WM31 dpy-17(e164) vab-7(e1562) III. Dpy and Vab (tail abnormalities).