| BA1013 |
spe-6(hc49) vab-7(e1562)/qC1 [dpy-19(e1259) glp-1(q339)] III; spe-27(it132) IV. |
Male/hermaphrodite line. Maintain at 15C to insure maintenance of male/hermaphrodite line. |
| BA609 |
spe-6(hc49) vab-7(e1562) III; eDp6 (III;f). |
Animals with the Duplications are WT. Animals which have lost the Duplication are Sterile and have an abnormal tail. |
| CB1562 |
vab-7(e1562) III. |
Unc. Tail abnormalities-twisted or knobbed. Recessive. M-MATING-NO SUCCESS. |
| DA438 |
bli-4(e937) I; rol-6(e187) II; daf-2(e1368) vab-7(e1562) III; unc-31(e928) IV; dpy-11(e224) V; lon-2(e678) X. |
Linkage mapping strain. Maintain at 15C. |
| GE42 |
vab-7(e1562) pha-1(e2123) III. |
Temperature sensitive. Maintain at 15C. pha-1(e2123) is embryonic lethal at 25C. Hermaphrodites have an abnormal tail: sometimes twisted, tail whip never formed, often bobbed; sometimes uncoordinated with bent tail. Adult male tail deformed also. |
| HR492 |
+/hT2 I; vab-7(e1562) mel-44(sb44)/hT2 [bli-4(e937) let-?(h661)] III. |
Dominant ts maternal-effect embryonic lethal. Embryos arrest prior to morphogenesis or at two-fold. Non-ts recessive mid-larval lethal. Maintain at 15C. Freezes poorly. |
| OH15912 |
vab-7(ot959[vab-7::GFP::FLAG]) III. |
Superficially wild-type. Insertion of GFP and FLAG tags directly before the stop codon of endogenous vab-7 locus. Verified by sequencing. Reference: Reilly MB, et al. Nature. 2020 Aug;584(7822):595-601. PMID: 32814896. |
| RW12267 |
vab-7(st12267[vab-7::TY1::EGFP::3xFLAG]) III. |
CRISPR/Cas9 engineered tagged endogenous locus. |
| VC1611 |
+/mT1 II; vab-7(gk709)/mT1 [dpy-10(e128)] III. |
M142.4. Apparent homozygous lethal deletion chromosome balanced by dpy-10-marked translocation. Heterozygotes are WT, and segregate WT, arrested mT1 aneuploids, sterile Dpys (mT1 homozygotes), and gk709 homozygotes (arrest stage/phenotype undetermined). Pick WT and check for correct segregation of progeny to maintain. External left primer: CCTTCCCCTCTTCTATTGGC. External right primer: CGACACGCAACACCAATTAC. Internal left primer: AATCCACCGTAGTCACCGAG. Internal right primer: TCAGAGCATGTTTCCCAGTG. Internal WT amplicon: 2427 bp. Deletion size: 1019 bp. Deletion left flank: TAAATTTTTGTAGTTTTTTAGGGATTTTTT. Deletion right flank: TCACTTGTGGAGATGGTCGCCGCATCTCGA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807 |
| WM31 |
dpy-17(e164) vab-7(e1562) III. |
Dpy and Vab (tail abnormalities). |